ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MTARGAAGRC PSSTWMGSRL LLVCLLVSRS VAEVSEHCSH MIGNGHLQIL QQLIDSQMET 

        70         80         90        100        110        120 
ACLIEYKFVD QEQLDDPVCY LKKAFVLVQV IIEETMRFKD NTPNANATER LQELSMKLNS 

       130        140        150        160        170        180 
CFIKDYKEQN EACVQTYKES PLRLLEKIKN FFNETKNFLE KDWNIFSKNC NDSLAKCSSR 

       190        200        210        220        230        240 
DVVTKPDCNC LYPKATPSSD LASASPHQPP APSMAPLADL AWDDSQRTEG SSLLPSDLPL 

       250        260        270        280        290        300 
RIEDPGSAKQ RPPRSTCQTL ESTEQPNHED PQPHPSAGAP IPGVEDIIES SMGTNWVLEE 

       310        320        330        340        350        360 
ASGEASEGFL TQERKFSPSN PVGGSIQAET DRPWARSASS PFPKLTEDQQ PTNITDTPLT 

       370        380        390        400        410        420 
EVNPMRPTGQ TLNNTPEKTD GSSTLREDQQ EPRSPHFATL NPQRVGNSAT PYAKLLPPKS 

       430        440        450        460        470        480 
HSWGIVLPLG ELEGKKSTRD RRSPAELKGG PASEGAARPV AQSTRDRRSP AELKGGPASE 

       490        500        510        520        530        540 
GAARPVARFN SIPLTDTGSS IQDPQTSAFV FWVLGIILVL LAVGGLLFYS WKRRSHRDPR 

       550        560 
TLDSSVGRPE GSSLAQDEDR QVELPV 

References and documentation are available.

Number of amino acids: 566

Molecular weight: 62152.73

Theoretical pI: 5.45

Amino acid composition: 
Ala (A)  41	  7.2%
Arg (R)  34	  6.0%
Asn (N)  23	  4.1%
Asp (D)  31	  5.5%
Cys (C)  12	  2.1%
Gln (Q)  29	  5.1%
Glu (E)  42	  7.4%
Gly (G)  32	  5.7%
His (H)   9	  1.6%
Ile (I)  20	  3.5%
Leu (L)  53	  9.4%
Lys (K)  27	  4.8%
Met (M)   9	  1.6%
Phe (F)  16	  2.8%
Pro (P)  51	  9.0%
Ser (S)  60	 10.6%
Thr (T)  35	  6.2%
Trp (W)   8	  1.4%
Tyr (Y)   7	  1.2%
Val (V)  27	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 73
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2702
Hydrogen    H	      4293
Nitrogen    N	       773
Oxygen      O	       867
Sulfur      S	        21

Formula: C2702H4293N773O867S21
Total number of atoms: 8656

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55180
Abs 0.1% (=1 g/l)   0.888, assuming all pairs of Cys residues form cystines


Ext. coefficient    54430
Abs 0.1% (=1 g/l)   0.876, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 61.69
This classifies the protein as unstable.



Aliphatic index: 71.38

Grand average of hydropathicity (GRAVY): -0.596