The sequence to investigate:
10 20 30 40 50 60
MKWVTFISLL FLFSSAYSRG VFRRDAHKSE VAHRFKDLGE ENFKALVLIA FAQYLQQCPF
70 80 90 100 110 120
EDHVKLVNEV TEFAKTCVAD ESAENCDKSL HTLFGDKLCT VATLRETYGE MADCCAKQEP
130 140 150 160 170 180
ERNECFLQHK DDNPNLPRLV RPEVDVMCTA FHDNEETFLK KYLYEIARRH PYFYAPELLF
190 200 210 220 230 240
FAKRYKAAFT ECCQAADKAA CLLPKLDELR DEGKASSAKQ RLKCASLQKF GERAFKAWAV
250 260 270 280 290 300
ARLSQRFPKA EFAEVSKLVT DLTKVHTECC HGDLLECADD RADLAKYICE NQDSISSKLK
310 320 330 340 350 360
ECCEKPLLEK SHCIAEVEND EMPADLPSLA ADFVESKDVC KNYAEAKDVF LGMFLYEYAR
370 380 390 400 410 420
RHPDYSVVLL LRLAKTYETT LEKCCAAADP HECYAKVFDE FKPLVEEPQN LIKQNCELFE
430 440 450 460 470 480
QLGEYKFQNA LLVRYTKKVP QVSTPTLVEV SRNLGKVGSK CCNPEAKRMP CAEDYLSVVL
490 500 510 520 530 540
NQLCVLHEKT PVSDRVTKCC TESLVNRRPC FSALEVDETY VPKEFNAETF TFHADICTLS
550 560 570 580 590 600
EKERQIKKQT ALVELVKHKP KATKEQLKAV MDDFAAFVEK CCKADDKETC FAEEGKKLVA
ASQAALGL
The sequence is 608 amino acids long.
The enzyme(s) that you have chosen:
You have chosen to display all possible cleaving enzymes.
These enzymes cleave the sequence:
| Name of enzyme | No. of cleavages | Positions of cleavage sites |
|---|
| Trypsin | 79 | 2 19 23 24 28 34 36 44 65 75 88 97 105 117 122 130 138 160 161 168 183 184 186 198 205 210 214 219 221 223 229 233 236 242 246 249 257 264 281 286 298 300 310 337 341 347 360 372 375 383 396 413 426 434 437 438 452 456 460 467 468 489 495 498 507 523 542 544 547 548 557 561 564 568 580 583 587 596 597 |
These are the cleavage sites of the chosen enzymes and chemicals mapped onto the entered protein sequence:
- You have chosen a block size of 60 for the map.
- Please note that the cleavage occurs at the right side (C-terminal direction) of the marked amino acid.
- You have the possibility to display the results of a single enzyme by mouseclicking on the respective enzyme name in the map.
Tryps
Tryps |
Tryps| | Tryps
Tryps Tryps || | Tryps | Tryps
| | || | | | |
MKWVTFISLLFLFSSAYSRGVFRRDAHKSEVAHRFKDLGEENFKALVLIAFAQYLQQCPF
1 ---------+---------+---------+---------+---------+---------+ 60
Tryps Tryps Tryps Tryps Tryps Tryps
| | | | | |
EDHVKLVNEVTEFAKTCVADESAENCDKSLHTLFGDKLCTVATLRETYGEMADCCAKQEP
61 ---------+---------+---------+---------+---------+---------+ 120
Tryps
Tryps Tryps Tryps Tryps| Tryps
| | | || |
ERNECFLQHKDDNPNLPRLVRPEVDVMCTAFHDNEETFLKKYLYEIARRHPYFYAPELLF
121 ---------+---------+---------+---------+---------+---------+ 180
Tryps
Tryps |
Tryps | |
Tryps Tryps | | | Tryps
Tryps | Tryps | | | | Tryps |
Tryps| | Tryps Tryps | | | | | Tryps | |
|| | | | | | | | | | | |
FAKRYKAAFTECCQAADKAACLLPKLDELRDEGKASSAKQRLKCASLQKFGERAFKAWAV
181 ---------+---------+---------+---------+---------+---------+ 240
Tryps
Tryps | Tryps Tryps
Tryps | | Tryps Tryps Tryps | Tryps |
| | | | | | | | |
ARLSQRFPKAEFAEVSKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLK
241 ---------+---------+---------+---------+---------+---------+ 300
Tryps
Tryps Tryps | Tryps Tryps
| | | | |
ECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYAR
301 ---------+---------+---------+---------+---------+---------+ 360
Tryps
Tryps | Tryps Tryps Tryps
| | | | |
RHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFE
361 ---------+---------+---------+---------+---------+---------+ 420
Tryps Tryps
Tryps| Tryps | Tryps
Tryps Tryps || Tryps | | Tryps|
| | || | | | ||
QLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCNPEAKRMPCAEDYLSVVL
421 ---------+---------+---------+---------+---------+---------+ 480
Tryps
Tryps Tryps | Tryps Tryps
| | | | |
NQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLS
481 ---------+---------+---------+---------+---------+---------+ 540
Tryps Tryps
Tryps| Tryps | Tryps
Tryps || Tryps | | Tryps | Tryps
Tryps | || Tryps | | | Tryps | | Tryps|
| | || | | | | | | | ||
EKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVA
541 ---------+---------+---------+---------+---------+---------+ 600
ASQAALGL
601 -------- 608