Accession	MF_01351
Dates	23-JUN-2006 (Created) 30-JUN-2008 (Last updated, Version 26)

Data class

Protein

Predictors

HAMAP; MF_01351; [distribution of match scores in UniProtKB];[seed alignment for MF_01351]

Identifier

NDHI

Protein name

RecName: Full=NAD(P)H-quinone oxidoreductase subunit I, chloroplastic; EC=1.6.5.-; AltName: Full=NAD(P)H dehydrogenase subunit I; Short=NDH subunit I; AltName: Full=NADH-plastoquinone oxidoreductase subunit I;

Gene name

ndhI

Identifier

NDHI

Protein name

RecName: Full=NAD(P)H-quinone oxidoreductase subunit I; EC=1.6.5.-; AltName: Full=NAD(P)H dehydrogenase I subunit I; AltName: Full=NDH-1 subunit I; Short=NDH-I;

Gene name

ndhI

Identifier

NUOI

Protein name

RecName: Full=NADH-quinone oxidoreductase subunit I; EC=1.6.99.5; AltName: Full=NADH dehydrogenase I subunit I; AltName: Full=NDH-1 subunit I;

Gene name

nuoI

FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient (By similarity).

FUNCTION: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient (By similarity).

FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).

FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).

CATALYTIC ACTIVITY: NAD(P)H + plastoquinone = NAD(P)(+) + plastoquinol.

CATALYTIC ACTIVITY: NADH + quinone = NAD(+) + quinol.

COFACTOR: Binds 2 4Fe-4S clusters per subunit (By similarity).

SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus (By similarity).

SUBUNIT: NDH-1 is composed of at least 11 different subunits (By similarity).

SUBUNIT: NDH-1 is composed of 13 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex (By similarity).

SUBUNIT: NDH-1 is composed of 15 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex (By similarity).

SUBUNIT: NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein (By similarity).

SUBCELLULAR LOCATION: Cellular thylakoid membrane; Peripheral membrane protein (By similarity).

SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein (Potential).

SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein (Potential).

SIMILARITY: Belongs to the complex I 23 kDa subunit family.

Pfam	PF00037; Fer4; 2;
PRINTS	PR00353; 4FE4SFRDOXIN; 1;
PROSITE	PS00198; 4FE4S_FER_1; 2; PS51379; 4FE4S_FER_2; 2; trigger=PRU00711;

NAD, Oxidoreductase, Quinone, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Membrane.

Thylakoid.

Cell membrane, Cell inner membrane.

Cell membrane.

Plastoquinone, NADP.

Ubiquinone.

GO:0005506; Molecular function: iron ion binding.
GO:0055114; Biological process: oxidation reduction.

GO:0016655; Molecular function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor.

GO:0050136; Molecular function: NADH dehydrogenase (quinone) activity.

GO:0019684; Biological process: photosynthesis, light reaction.

GO:0009535; Cellular component: chloroplast thylakoid membrane.

GO:0042651; Cellular component: thylakoid membrane.

GO:0005886; Cellular component: plasma membrane.

GO:0005886; Cellular component: plasma membrane.

From: NQO9_THET8 (Q56224)
Key		From		To		Description		Condition		FTGroup
METAL		53		53		Iron-sulfur 1 (4Fe-4S) (By similarity)		C		1
METAL		56		56		Iron-sulfur 1 (4Fe-4S) (By similarity)		C		1
METAL		59		59		Iron-sulfur 1 (4Fe-4S) (By similarity)		C		1
METAL		63		63		Iron-sulfur 2 (4Fe-4S) (By similarity)		C		2
METAL		98		98		Iron-sulfur 2 (4Fe-4S) (By similarity)		C		2
METAL		101		101		Iron-sulfur 2 (4Fe-4S) (By similarity)		C		2
METAL		104		104		Iron-sulfur 2 (4Fe-4S) (By similarity)		C		2
METAL		108		108		Iron-sulfur 1 (4Fe-4S) (By similarity)		C		1

Size range:	131-264 amino acids
Related UniRules:	None
Template:	Q56224 (NQO9_THET8); P0AFD6 (NUOI_ECOLI): [Recover all]
Scope:	Bacteria Plastid
Fusion:	Nter: MF_01350 (nuoH); Cter: None
Duplicate:	in ANADE, AQUAE, GEOMG, GEOSL, MYCPA, NITMU, NITOC, PELPD, RHIEC, RHIME, RHOP2, RHOP5, RHOPA, RHOPB, RHOPS, RHOS1, RHOS4, SOLUE, STRAW, STRCO, SYMTH, SYNFM
Plasmid encoded:	None
Comments:	In NOCFA is N-terminally fused with nuoH, another subunit of the same complex. See also comments on subunit composition in family MF_01350 (nuoH/ndhA). Thermus bacteria are annotated with another nomenclature.