Accession	MF_01338
Dates	5-AUG-2005 (Created) 7-JUN-2008 (Last updated, Version 25)

Data class

Protein

Predictors

HAMAP; MF_01338; [distribution of match scores in UniProtKB];[seed alignment for MF_01338]

Identifier

RBL

Protein name

RecName: Full=Ribulose bisphosphate carboxylase large chain; Short=RuBisCO large subunit; EC=4.1.1.39; Flags: Precursor;

Gene name

rbcL

Protein name

RecName: Full=Ribulose bisphosphate carboxylase large chain; Short=RuBisCO large subunit; EC=4.1.1.39;

Gene name

rbcL

Protein name

RecName: Full=Ribulose bisphosphate carboxylase large chain; Short=RuBisCO large subunit; EC=4.1.1.39;

Gene name

cbbL, rbcL

Protein name

RecName: Full=Ribulose bisphosphate carboxylase large chain; Short=RuBisCO large subunit; EC=4.1.1.39;

Gene name

cbbL

FUNCTION: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site (By similarity).

FUNCTION: RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site (By similarity).

CATALYTIC ACTIVITY: 2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O.

CATALYTIC ACTIVITY: 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O(2).

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

SUBUNIT: Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers (By similarity).

SUBUNIT: Heterohexadecamer of 8 large chains and 8 small chains (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

PTM: The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover (By similarity).

MISCELLANEOUS: The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" (By similarity).

SIMILARITY: Belongs to the RuBisCO large chain family. Type I subfamily.

PROSITE	PS00157; RUBISCO_LARGE; 1;
Pfam	PF00016; RuBisCO_large; 1; PF02788; RuBisCO_large_N; 1;

Acetylation.

Calvin cycle, Carbon dioxide fixation, Lyase.

Magnesium, Metal-binding.

Methylation.

Monooxygenase, Oxidoreductase.

Photorespiration.

Photosynthesis.

GO:0000287; Molecular function: magnesium ion binding.

GO:0016984; Molecular function: ribulose-bisphosphate carboxylase activity.

GO:0009507; Cellular component: chloroplast.

From: RBL_TOBAC (P00876)
Key		From		To		Description		Condition		FTGroup
PROPEP		1		2		By similarity
CHAIN		3		Cter		Ribulose bisphosphate carboxylase large chain

Key		From		To		Description		Condition		FTGroup
SITE		334		334		Transition state stabilizer (By similarity)		K
ACT_SITE		175		175		Proton acceptor (By similarity)		K
ACT_SITE		294		294		Proton acceptor (By similarity)		H
METAL		201		201		Magnesium (via carbamate group) (By similarity)		K		1
METAL		203		203		Magnesium (By similarity)		D		1
METAL		204		204		Magnesium (By similarity)		E		1
BINDING		123		123		Substrate; in homodimeric partner (By similarity)		N
BINDING		173		173		Substrate (By similarity)		T
BINDING		177		177		Substrate (By similarity)		K
BINDING		295		295		Substrate (By similarity)		R
BINDING		327		327		Substrate (By similarity)		H
BINDING		379		379		Substrate (By similarity)		S

Key		From		To		Description		Condition		FTGroup
MOD_RES		3		3		N-acetylproline (By similarity)		P
MOD_RES		14		14		N6,N6,N6-trimethyllysine (By similarity)		K

Key		From		To		Description		Condition		FTGroup
MOD_RES		201		201		N6-carboxylysine (By similarity)		K
DISULFID (Optional)		247		247		Interchain; in linked form (By similarity)		C

Size range:	463-499 amino acids
Related UniRules:	MF_01133 (RBL); MF_01339 (RBL2)
Template:	P00876 (RBL_TOBAC); P0C2C2 (RBL1C_RALEU); P00880 (RBL_SYNP6); P00877 (RBL_CHLRE); P0C512 (RBL_ORYSJ); P00875 (RBL_SPIOL): [Recover all]
Scope:	Bacteria; Cyanobacteria Bacteria; Alphaproteobacteria Bacteria; Betaproteobacteria Bacteria; Acidithiobacillales Bacteria; Chromatiales Bacteria; Methylococcales Bacteria; Thiotrichales Bacteria; sulfur-oxidizing symbionts Plastid
Fusion:	Nter: None; Cter: None
Duplicate:	in BRASB, BRASO, CHRVI, HYDMR, METPP, NITSX, RALEU, THIFE
Plasmid encoded:	in OLICA, RALEU, RHIME