HAMAP rule MF_00970
General rule information
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Accession | MF_00970 |
Dates | 7-AUG-2013 (Created)
1-JUN-2023 (Last updated, Version 12) |
Name | RNase_E |
Scope(s) |
Bacteria Pseudomonadota |
Template(s) | P21513 (RNE_ECOLI); [ Recover all ] |
Triggered by |
HAMAP; MF_00970 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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Identifier | RNE |
Protein name | RecName: Full=Ribonuclease E; Short=RNase E; EC=3.1.26.12; |
Gene name | Name=rne; |
Comments
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FUNCTION | Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs. |
CATALYTIC ACTIVITY | Reaction=Endonucleolytic cleavage of single-stranded RNA in A- and U- rich regions.; EC=3.1.26.12; |
case <FTGroup:2> | |
COFACTOR | Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 Zn(2+) ions per homotetramer.; |
end case | |
case <FTGroup:1> | |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.; |
end case | |
case <OC:Gammaproteobacteria> | |
SUBUNIT | Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers. |
else | |
SUBUNIT | Homotetramer formed by a dimer of dimers. |
end case | |
SUBCELLULAR LOCATION | Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. |
SIMILARITY | Belongs to the RNase E/G family. RNase E subfamily. |
Keywords
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Cell inner membrane | |
Cell membrane | |
Cytoplasm | |
Endonuclease | |
Hydrolase | |
case <FTGroup:1> | |
Magnesium | |
end case | |
Membrane | |
case <FTGroup:1> or <FTGroup:2> | |
Metal-binding | |
end case | |
Nuclease | |
RNA-binding | |
rRNA processing | |
tRNA processing | |
rRNA-binding | |
tRNA-binding | |
case <FTGroup:2> | |
Zinc | |
end case |
Gene Ontology
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GO:0004521; Molecular function:RNA endonuclease activity | |
case <FTGroup:1> | |
GO:0000287; Molecular function:magnesium ion binding | |
end case | |
GO:0003723; Molecular function:RNA binding | |
case <FTGroup:2> | |
GO:0008270; Molecular function:zinc ion binding | |
end case | |
GO:0006402; Biological process:mRNA catabolic process | |
GO:0006364; Biological process:rRNA processing | |
GO:0008033; Biological process:tRNA processing | |
GO:0005737; Cellular component:cytoplasm | |
GO:0009898; Cellular component:cytoplasmic side of plasma membrane |
Cross-references
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PROSITE | PS50126; S1; 1; |
Pfam | PF12111; PNPase_C; 0-1; |
Pfam | PF10150; RNase_E_G; 1; |
Pfam | PF00575; S1; 1; |
NCBIfam | TIGR00757; RNaseEG; 1; |
Features
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From: RNE_ECOLI (P21513) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
REGION | 404 | 407 | /note="Required for zinc-mediated homotetramerization and catalytic activity" | C-x-x-C | ||||||||
BINDING | 303 | 303 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic" |
D | 1 | |||||||
BINDING | 346 | 346 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" /ligand_note="catalytic" |
D | 1 | |||||||
BINDING | 404 | 404 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners" |
C | 2 | |||||||
BINDING | 407 | 407 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" /ligand_note="ligand shared between dimeric partners" |
C | 2 |
Additional information
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Size range | 560-1230 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |