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HAMAP rule MF_00449

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General rule information [?]

Accession MF_00449
Dates 17-SEP-2001 (Created)
19-NOV-2022 (Last updated, Version 31)
Name RAD50
Scope(s) Archaea
Template(s) P58301 (RAD50_PYRFU); [ Recover all ]
Triggered by HAMAP; MF_00449 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RAD50
Protein name RecName: Full=DNA double-strand break repair Rad50 ATPase;
Gene name Name=rad50;

Comments [?]

case <OC:Halobacteria>
FUNCTIONPart of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.
else
FUNCTIONPart of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.
end case
case <FTGroup:1>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 zinc ion per homodimer.;
DOMAINThe two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.
end case
SUBUNITHomodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.
SIMILARITYBelongs to the SMC family. RAD50 subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0016887; Molecular function:ATP hydrolysis activity
GO:0006302; Biological process:double-strand break repair
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case

Cross-references [?]

PROSITE PS51131; ZN_HOOK; 1;
Pfam PF04423; Rad50_zn_hook; 1;
Pfam PF02463; SMC_N; 1;
General Coiled_coil; -; 0-unlimited;

Features [?]

From: RAD50_PYRFU (P58301)
Key From To Description Tag Condition FTGroup
BINDING 32 38 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
N-[GA]-[SA]-G-K-[ST]-[ST]
BINDING 791 796 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[FLIM]-[LIVFM]-S-G-G-E
BINDING 444 444 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
C 1
BINDING 447 447 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
C 1
BINDING 12 12 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[RK]
BINDING 140 140 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
Q

Additional information [?]

Size range 830-1100 amino acids
Related rules None
Fusion Nter: None Cter: None



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