HAMAP rule MF_00185
General rule information
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| Accession | MF_00185 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 34) |
| Name | IPP_trans |
| Scope(s) |
Bacteria |
| Template(s) | P16384 (MIAA_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00185 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | MIAA |
| Protein name | RecName: Full=tRNA dimethylallyltransferase; EC=2.5.1.75; AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase; Short=DMAPP:tRNA dimethylallyltransferase; Short=DMATase; AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase; Short=IPP transferase; Short=IPPT; Short=IPTase; |
| Gene name | Name=miaA; |
Comments
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| FUNCTION | Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A). |
| CATALYTIC ACTIVITY | Reaction=adenosine(37) in tRNA + dimethylallyl diphosphate = diphosphate + N(6)-dimethylallyladenosine(37) in tRNA; Xref=Rhea:RHEA:26482, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10375, ChEBI:CHEBI:33019, ChEBI:CHEBI:57623, ChEBI:CHEBI:74411, ChEBI:CHEBI:74415; EC=2.5.1.75; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Monomer. |
| SIMILARITY | Belongs to the IPP transferase family. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0052381; Molecular function:tRNA dimethylallyltransferase activity |
| GO:0008033; Biological process:tRNA processing |
Cross-references
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Features
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| From: MIAA_ECOLI (P16384) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 17 | 24 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
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| BINDING | 19 | 24 | /ligand="substrate" | [ST]-x-x-[GN]-K-[ST] | ||||||||
| REGION | 42 | 45 | /note="Interaction with substrate tRNA" | D-S-x-x | ||||||||
| REGION | 166 | 170 | /note="Interaction with substrate tRNA" | Q-R-x-x-R | ||||||||
| REGION | 247 | 252 | /note="Interaction with substrate tRNA" | R-C-V-G-Y-R | ||||||||
| REGION | 280 | 287 | /note="Interaction with substrate tRNA" | K-R-Q-I-T-W-L-R | ||||||||
| SITE | 108 | 108 | /note="Interaction with substrate tRNA" | [TS] | ||||||||
| SITE | 130 | 130 | /note="Interaction with substrate tRNA" | R | ||||||||
Additional information
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| Size range | 279-363 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Possible wrong start in a number of cases |