HAMAP rule MF_00178
General rule information
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| Accession | MF_00178 |
| Dates | 1-JUN-2001 (Created)
1-JUN-2023 (Last updated, Version 37) |
| Name | Lumazine_synth |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P61714 (RISB_ECOLI); O66529 (RISB_AQUAE); P11998 (RISB_BACSU); P61711 (RISB2_BRUAB); P9WHE9 (RISB_MYCTU); Q81MB5 (RISB_BACAN); Q57DY1 (RISB1_BRUAB); Q986N2 (RISB2_RHILO); Q983B0 (RISB1_RHILO); Q8YGH2 (RISB1_BRUME); Q57751 (RISB_METJA); P66038 (RISB_SALTY); Q2YKV1 (RISB2_BRUA2); Q2YNC6 (RISB1_BRUA2); [ Recover all ] |
| Triggered by |
HAMAP; MF_00178 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | RISB |
| Protein name | RecName: Full=6,7-dimethyl-8-ribityllumazine synthase; Short=DMRL synthase; Short=Lumazine synthase; Short=LS; EC=2.5.1.78; |
| Gene name | Name=ribH; |
Comments
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| FUNCTION | Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2- butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. |
| CATALYTIC ACTIVITY | Reaction=(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D- ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) + 2 H2O + phosphate; Xref=Rhea:RHEA:26152, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15934, ChEBI:CHEBI:43474, ChEBI:CHEBI:58201, ChEBI:CHEBI:58830; EC=2.5.1.78; |
| PATHWAY | Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D- ribitylamino)uracil: step 1/2. |
| case <OC:Aquificae> or <OC:Bacillales> or <OC:Gammaproteobacteria> or <OC:Methanococci> | |
| SUBUNIT | Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers. |
| else case <OC:Mycobacterium> | |
| SUBUNIT | Homopentamer. |
| end case | |
| SIMILARITY | Belongs to the DMRL synthase family. |
Keywords
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Gene Ontology
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| GO:0000906; Molecular function:6,7-dimethyl-8-ribityllumazine synthase activity |
| GO:0009231; Biological process:riboflavin biosynthetic process |
Cross-references
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Features
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| From: RISB_AQUAE (O66529) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 56 | 58 | /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934" |
|||||||||
| BINDING | 80 | 82 | /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934" |
|||||||||
| BINDING | 85 | 86 | /ligand="(2S)-2-hydroxy-3-oxobutyl phosphate" /ligand_id="ChEBI:CHEBI:58830" |
[AQSGEDKTN]-[TS] | ||||||||
| ACT_SITE | 88 | 88 | /note="Proton donor" | [HR] | ||||||||
| BINDING | 22 | 22 | /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934" |
[FWY] | ||||||||
| BINDING | 113 | 113 | /ligand="5-amino-6-(D-ribitylamino)uracil" /ligand_id="ChEBI:CHEBI:15934" |
[FNLSDMYIHV] | ||||||||
| BINDING | 127 | 127 | /ligand="(2S)-2-hydroxy-3-oxobutyl phosphate" /ligand_id="ChEBI:CHEBI:58830" |
[RH] | ||||||||
Additional information
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| Size range | 133-190 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Lumazine synthase structures are either observed as pentamers, dimers of pentamers or dodecamers of pentamers. |