[1]	PARTIAL PROTEIN SEQUENCE. TISSUE=Pancreas; DOI=10.1016/0014-5793(75)80120-7; PubMed=1157925 [NCBI, ExPASy, EBI, Israel, Japan] Leijenaar-Van den Berg G., Beintema J.J.; "The amino acid sequences of reindeer, moose and fallow deer pancreatic ribonucleases."; FEBS Lett. 56:101-107(1975).

Comments

FUNCTION: Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA (By similarity).
CATALYTIC ACTIVITY: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Pancreas.
SIMILARITY: Belongs to the pancreatic ribonuclease family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

PIR

B91418; NRDEN.

3D structure databases

HSSP

P00656; 1SRN. [HSSP ENTRY / SWISS-3DIMAGE / PDB]

SMR

P00666; 1-124.

ModBase

P00666.

Enzyme and pathway databases

BRENDA

3.1.27.5; 289587.

Ontologies

GO:0005576;	Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-SubCell).
GO:0003676;	Molecular function: nucleic acid binding (inferred from electronic annotation from InterPro).
GO:0004522;	Molecular function: pancreatic ribonuclease activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001427; RNaseA.
Graphical view of domain structure.

Gene3D

G3DSA:3.10.130.10; RNaseA; 1.

PANTHER

PTHR11437; RNaseA; 1.

Pfam

PF00074; RnaseA; 1.
Pfam graphical view of domain structure.

PRINTS

PR00794; RIBONUCLEASE.

ProDom

PD000535; RNaseA; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00092; RNAse_Pc; 1.
SMART graphical view of domain structure.

PROSITE

PS00127; RNASE_PANCREATIC; 1.

Phylogenomic databases

HOVERGEN

P00666; -.

Other

ProtoNet

P00666.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Disulfide bond; Endonuclease; Hydrolase; Nuclease; Secreted.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 124`	`124`	`Ribonuclease pancreatic.`	`PRO_0000057213`
`REGION`	`41 45`	`5`	`Substrate binding (By similarity).`
`ACT_SITE`	`12 12`		`Proton acceptor (By similarity).`
`ACT_SITE`	`119 119`		`Proton donor (By similarity).`
`BINDING`	`7 7`		`Substrate (By similarity).`
`BINDING`	`10 10`		`Substrate (By similarity).`
`BINDING`	`66 66`		`Substrate (By similarity).`
`BINDING`	`85 85`		`Substrate (By similarity).`
`DISULFID`	`26 84`		`By similarity.`
`DISULFID`	`40 95`		`By similarity.`
`DISULFID`	`58 110`		`By similarity.`
`DISULFID`	`65 72`		`By similarity.`

Sequence information

Length: 124 AA [This is the length of the unprocessed precursor]

Molecular weight: 13759 Da [This is the MW of the unprocessed precursor]

CRC64: CE56B8BB9A87CD54 [This is a checksum on the sequence]

        10         20         30         40         50         60 
KESAAAKFER QHMDPSPSSA SSSNYCNQMM QSRDLTQDRC KPVNTFVHES LADVQAVCFQ 

        70         80         90        100        110        120 
KNVACKNGQS NCYQSNSAMH ITDCRETGSS KYPNCVYKTT QAEKHIIVAC EGNPYVPVHF 


DASV

P00666 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools