[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND MUTAGENESIS OF 1165-ASN--CYS-1168.
DOI=10.1101/gad.989402; PubMed=12130538 [NCBI, ExPASy, EBI, Israel, Japan]
Tachibana M.,
Sugimoto K.,
Nozaki M.,
Ueda J.,
Ohta T.,
Ohki M.,
Fukuda M.,
Takeda N.,
Niida H.,
Kato H.,
Shinkai Y.;
"G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis.";
Genes Dev. 16:1779-1791(2002).
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=129;
DOI=10.1101/gr.1736803; PubMed=14656967 [NCBI, ExPASy, EBI, Israel, Japan]
Xie T.,
Rowen L.,
Aguado B.,
Ahearn M.E.,
Madan A.,
Qin S.,
Campbell R.D.,
Hood L.;
"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.";
Genome Res. 13:2621-2636(2003).
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[3]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
TISSUE=Mammary tumor;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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[4]
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ALTERNATIVE SPLICING (ISOFORM 2).
DOI=10.1007/s00335-001-3029-3; PubMed=11707778 [NCBI, ExPASy, EBI, Israel, Japan]
Brown S.E.,
Campbell R.D.,
Sanderson C.M.;
"Novel NG36/G9a gene products encoded within the human and mouse MHC class III regions.";
Mamm. Genome 12:916-924(2001).
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[5]
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ENZYME ACTIVITY, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-1162.
DOI=10.1074/jbc.M101914200; PubMed=11316813 [NCBI, ExPASy, EBI, Israel, Japan]
Tachibana M.,
Sugimoto K.,
Fukushima T.,
Shinkai Y.;
"Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3.";
J. Biol. Chem. 276:25309-25317(2001).
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[6]
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INTERACTION WITH WIZ AND EHMT1.
DOI=10.1074/jbc.M603087200; PubMed=16702210 [NCBI, ExPASy, EBI, Israel, Japan]
Ueda J.,
Tachibana M.,
Ikura T.,
Shinkai Y.;
"Zinc finger protein Wiz links G9a/GLP histone methyltransferases to the co-repressor molecule CtBP.";
J. Biol. Chem. 281:20120-20128(2006).
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[7]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-608, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1073/pnas.0609836104; PubMed=17242355 [NCBI, ExPASy, EBI, Israel, Japan]
Villen J.,
Beausoleil S.A.,
Gerber S.A.,
Gygi S.P.;
"Large-scale phosphorylation analysis of mouse liver.";
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
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- FUNCTION: Histone methyltransferase. Preferentially methylates 'Lys-9' of histone H3 and 'Lys-27' of histone H3 (in vitro). H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also methylates histone H1.
- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine.
- SUBUNIT: Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, GFI1B, L3MBTL2 and YAF2 (By similarity). Interacts with WIZ and EHMT1.
- SUBCELLULAR LOCATION: Nucleus. Note=Almost excluded form nucleoli. Associates with euchromatic regions. Does not associate with heterochromatin.
- ALTERNATIVE PRODUCTS:
3 named isoforms [FASTA] produced by alternative splicing.
- DOMAIN: The SET domain mediates interaction with WIZ.
- MISCELLANEOUS: G9A deficient mice show a higher level of histone H3 with acetylated 'Lys-9' and/or methylated 'Lys-4', display severe developmental defects and die within E9.5-E12.5 stages.
- SIMILARITY: Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
- SIMILARITY: Contains 7 ANK repeats.
- SIMILARITY: Contains 1 post-SET domain.
- SIMILARITY: Contains 1 pre-SET domain.
- SIMILARITY: Contains 1 SET domain.
- CAUTION: NG36 and G9a were originally thought to derive from two separate genes.
- SEQUENCE CAUTION:
- Sequence=AAC84164.1; Type=Erroneous gene model prediction;
- Sequence=AAC84165.1; Type=Erroneous gene model prediction;
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