ID   MDHM_SCHPO              Reviewed;         341 AA.
AC   Q9Y7R8;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-NOV-2008, entry version 46.
DE   RecName: Full=Malate dehydrogenase, mitochondrial;
DE            EC=1.1.1.37;
DE   Flags: Precursor;
GN   Name=MDH1; ORFNames=SPCC306.08c;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the
RT   fission yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
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DR   EMBL; CU329672; CAB41656.1; -; Genomic_DNA.
DR   PIR; T41286; T41286.
DR   RefSeq; NP_587816.1; -.
DR   HSSP; P00346; 1MLD.
DR   GeneID; 2538766; -.
DR   KEGG; spo:SPCC306.08c; -.
DR   NMPDR; fig|4896.1.peg.154; -.
DR   GeneDB_Spombe; SPCC306.08c; -.
DR   ArrayExpress; Q9Y7R8; -.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0033554; P:cellular response to stress; IEP:GeneDB_SPombe.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR010097; Malate_DHase_NAD-dep_euk_g_bac.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11540:SF1; MDH_euk_g_bac; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Mitochondrion; NAD; Oxidoreductase;
KW   Transit peptide; Tricarboxylic acid cycle.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    341       Malate dehydrogenase, mitochondrial.
FT                                /FTId=PRO_0000310436.
FT   NP_BIND      35     41       NAD (By similarity).
FT   ACT_SITE    205    205       Proton acceptor (By similarity).
FT   BINDING      61     61       NAD (By similarity).
FT   BINDING     109    109       Substrate (By similarity).
FT   BINDING     115    115       Substrate (By similarity).
FT   BINDING     122    122       NAD (By similarity).
FT   BINDING     147    147       Substrate (By similarity).
FT   BINDING     181    181       Substrate (By similarity).
FT   BINDING     254    254       NAD (By similarity).
SQ   SEQUENCE   341 AA;  35787 MW;  4681EBFD2B1F7716 CRC64;
     MFAKVAFKNF TPLKSIAPRS FSTTSSRAFK VAVLGAGGGI GQPLSMLLKL NDKVSELALF
     DIRGAPGVAA DIGHINTTSN VVGYAPDDKG LEKALNGADV VIIPAGVPRK PGMTRDDLFA
     TNASIVRDLA FAAGETCPEA KYLVVTNPVN STVPIFKKAL ERVGVHQPKH LFGVTTLDSV
     RASRFTSQVT NGKAELLHIP VVGGHSGATI VPLLSQGGVE LTGEKRDALI HRIQFGGDEV
     VKAKAGAGSA TLSMAYAGAR MASSVLRALA GESGVEECTF VESPLYKDQG IDFFASRVTL
     GKDGVDTIHP VGKINDYEES LLKVALGELK KSITKGEQFV A
//