[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), AND TISSUE SPECIFICITY.
TISSUE=Retina;
DOI=10.1006/bbrc.1998.8658; PubMed=9610397 [NCBI, ExPASy, EBI, Israel, Japan]
Ishibashi K.,
Sasaki S.,
Marumo F.;
"Molecular cloning of a new sodium bicarbonate cotransporter cDNA from human retina.";
Biochem. Biophys. Res. Commun. 246:535-538(1998).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND REGULATION.
TISSUE=Kidney, and Skeletal muscle;
DOI=10.1074/jbc.274.23.16569; PubMed=10347222 [NCBI, ExPASy, EBI, Israel, Japan]
Pushkin A.,
Abuladze N.,
Lee I.,
Newman D.,
Hwang J.,
Kurtz I.;
"Cloning, tissue distribution, genomic organization, and functional characterization of NBC3, a new member of the sodium bicarbonate cotransporter family.";
J. Biol. Chem. 274:16569-16575(1999).
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[3]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
TISSUE=Neuroepithelium;
Romero M.F.;
"Cloning of a HCO3 transporter, NT2-NBC, from human brain, similar to both the Anion exchangers (AEs) and the Na/Bicarbonate Cotransporters (NBCs).";
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
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[4]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
TISSUE=Cervix;
The German cDNA consortium;
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
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[5]
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FUNCTION, INTERACTION WITH CFTR AND SLC9A3R1, AND DOMAIN.
DOI=10.1074/jbc.M201862200; PubMed=12403779 [NCBI, ExPASy, EBI, Israel, Japan]
Park M.,
Ko S.B.H.,
Choi J.Y.,
Muallem G.,
Thomas P.J.,
Pushkin A.,
Lee M.-S.,
Kim J.Y.,
Lee M.G.,
Muallem S.,
Kurtz I.;
"The cystic fibrosis transmembrane conductance regulator interacts with and regulates the activity of the HCO3- salvage transporter human Na+-HCO3-cotransport isoform 3.";
J. Biol. Chem. 277:50503-50509(2002).
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[6]
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INTERACTION WITH ATP6V1B1 AND SLC9A3R1, AND MUTAGENESIS OF LEU-1214.
DOI=10.1152/ajpcell.00225.2002; PubMed=12444018 [NCBI, ExPASy, EBI, Israel, Japan]
Pushkin A.,
Abuladze N.,
Newman D.,
Muronets V.,
Sassani P.,
Tatishchev S.,
Kurtz I.;
"The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction.";
Am. J. Physiol. 284:C667-C673(2003).
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[7]
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INTERACTION WITH CA2, REGULATION, AND MUTAGENESIS OF 1135-ASP-ASP-1136 AND 1163-ASP--ASP-1165.
DOI=10.1152/ajpcell.00382.2003; PubMed=14736710 [NCBI, ExPASy, EBI, Israel, Japan]
Loiselle F.B.,
Morgan P.E.,
Alvarez B.V.,
Casey J.R.;
"Regulation of the human NBC3 Na+/HCO3- cotransporter by carbonic anhydrase II and PKA.";
Am. J. Physiol. 286:C1423-C1433(2004).
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[8]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1213, AND MASS SPECTROMETRY.
TISSUE=T-cell;
DOI=10.1021/ac035352d; PubMed=15144186 [NCBI, ExPASy, EBI, Israel, Japan]
Brill L.M.,
Salomon A.R.,
Ficarro S.B.,
Mukherji M.,
Stettler-Gill M.,
Peters E.C.;
"Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry.";
Anal. Chem. 76:2763-2772(2004).
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[9]
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INTERACTION WITH USH1C.
DOI=10.1093/hmg/ddi417; PubMed=16301216 [NCBI, ExPASy, EBI, Israel, Japan]
Reiners J.,
van Wijk E.,
Maerker T.,
Zimmermann U.,
Juergens K.,
te Brinke H.,
Overlack N.,
Roepman R.,
Knipper M.,
Kremer H.,
Wolfrum U.;
"Scaffold protein harmonin (USH1C) provides molecular links between Usher syndrome type 1 and type 2.";
Hum. Mol. Genet. 14:3933-3943(2005).
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[10]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1167 AND SER-1176, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T.,
Yi W.,
Lu B.,
Park S.K.,
Xu T.,
Lee J.-D.,
Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
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- FUNCTION: Electroneutral sodium- and bicarbonate-dependent cotransporter with a Na(+):HCO3(-) 1:1 stoichiometry. Regulates intracellular pH and may play a role in bicarbonate salvage in secretory epithelia. May also have an associated sodium channel activity.
- ENZYME REGULATION: Transporter activity is regulated by CA2/carbonic anhydrase 2, cAMP and PKA. Insensitive to stilbene derivatives. PubMed:10347222 states it is inhibited by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA).
- BIOPHYSICOCHEMICAL PROPERTIES:
- SUBUNIT: Interacts with CFTR through SLC9A3R1/EBP50. Interacts with USH1C. Forms a complex with ATP6V1B1 and SLC9A3R1/EBP50. Interacts in a pH dependent-manner with CA2/carbonic anhydrase 2.
- INTERACTION:
P31946:YWHAB; NbExp=1; IntAct=EBI-1044546, EBI-359815;
- SUBCELLULAR LOCATION: Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein (By similarity). Cell projection, stereocilium (By similarity). Note=Also described at the apical cell membrane. Localizes to the stereocilia of cochlear outer hair cells and to the lateral membrane of cochlear inner hair cells (By similarity).
- ALTERNATIVE PRODUCTS:
5 named isoforms [FASTA] produced by alternative splicing.
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| Name | 2 |
| Isoform ID | Q9Y6M7-2 |
| Features which should be applied to build the isoform sequence: VSP_017162. |
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- TISSUE SPECIFICITY: Highly expressed in testis and spleen. Also expressed in retina, colon, small intestine, ovary, thymus, prostate, muscle, heart and kidney. Isoform 1 is expressed in skeletal muscle and heart muscle.
- DOMAIN: The PDZ-binding motif mediates interaction with the CFTR, SLC9A3R1/EBP50 complex and probably with USH1C.
- PTM: N-glycosylated (By similarity).
- SIMILARITY: Belongs to the anion exchanger (TC 2.A.31) family [view classification].
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