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UniProtKB/Swiss-Prot entry Q9Y385


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name UB2J1_HUMAN
Primary accession number Q9Y385
Secondary accession numbers Q5W0N4 Q9BZ32 Q9NQL3 Q9NY66 Q9P011 Q9P0S0 Q9UF10
Integrated into Swiss-Prot on September 13, 2004
Sequence was last modified on September 13, 2004 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 66)
Name and origin of the protein
Protein name Ubiquitin-conjugating enzyme E2 J1
Synonyms EC 6.3.2.19
Non-canonical ubiquitin-conjugating enzyme 1
NCUBE1
Yeast ubiquitin-conjugating enzyme UBC6 homolog E
HSUBC6e
Gene name
Name: UBE2J1
Synonyms: NCUBE1
ORFNames: CGI-76, HSPC153, HSPC205
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1006/bbrc.2000.2302; PubMed=10708578 [NCBI, ExPASy, EBI, Israel, Japan]
Lester D.H., Farquharson C., Russell G.C., Houston B.;
"Identification of a family of non-canonical ubiquitin-conjugating enzymes structurally related to yeast UBC6.";
Biochem. Biophys. Res. Commun. 269:474-480(2000).
[2]
NUCLEOTIDE SEQUENCE [MRNA], VARIANT VAL-55, FUNCTION, MUTAGENESIS OF CYS-91, AND SUBCELLULAR LOCATION.
PubMed=12082160 [NCBI, ExPASy, EBI, Israel, Japan]
Lenk U., Yu H., Walter J., Gelman M.S., Hartmann E., Kopito R.R., Sommer T.;
"A role for mammalian Ubc6 homologues in ER-associated protein degradation.";
J. Cell Sci. 115:3007-3014(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.10.5.703; PubMed=10810093 [NCBI, ExPASy, EBI, Israel, Japan]
Lai C.-H., Chou C.-Y., Ch'ang L.-Y., Liu C.-S., Lin W.-C.;
"Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics.";
Genome Res. 10:703-713(2000).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-229.
TISSUE=Umbilical cord blood;
DOI=10.1101/gr.140200; PubMed=11042152 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang Q.-H., Ye M., Wu X.-Y., Ren S.-X., Zhao M., Zhao C.-J., Fu G., Shen Y., Fan H.-Y., Lu G., Zhong M., Xu X.-R., Han Z.-G., Zhang J.-W., Tao J., Huang Q.-H., Zhou J., Hu G.-X., Gu J., Chen S.-J., Chen Z.;
"Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.";
Genome Res. 10:1546-1560(2000).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature02055; PubMed=14574404 [NCBI, ExPASy, EBI, Israel, Japan]
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.;
"The DNA sequence and analysis of human chromosome 6.";
Nature 425:805-811(2003).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-229.
TISSUE=Muscle;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266 AND SER-268, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1021/pr070152u; PubMed=17924679 [NCBI, ExPASy, EBI, Israel, Japan]
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.;
"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.";
J. Proteome Res. 6:4150-4162(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ245898; CAB83212.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U93243; AAF21505.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF151039; AAF36125.1; ALT_FRAME; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF151834; AAD34071.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF161502; AAF29117.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL138717; CAH70228.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL139804; CAH70228.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL139804; CAI19635.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL138717; CAI19635.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC013973; AAH13973.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_057105.2; -.
UniGene Hs.163776
3D structure databases
HSSP P15731; 1QCQ. [HSSP ENTRY / PDB]
ModBase Q9Y385.
Protein-protein interaction databases
IntAct Q9Y385; -.
PTM databases
PhosphoSite Q9Y385; -.
2D gel databases
OGP Q9Y385; -.
Organism-specific databases
H-InvDB HIX0023025; -.
HGNC HGNC:17598; UBE2J1.
GenAtlas UBE2J1.
HPA HPA003509; -.
PharmGKB PA134906541; -.
GeneCards Q9Y385.
Gene expression databases
ArrayExpress Q9Y385; -.
CleanEx HS_UBE2J1; -.
GermOnline ENSG00000198833; Homo sapiens.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR016135; UBQ-conjugat/RWD-like.
IPR000608; UBQ-conjugat_E2.
Graphical view of domain structure.
Gene3D G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
PANTHER PTHR11621; UBQ-conjugat_E2; 1.
Pfam PF00179; UQ_con; 1.
Pfam graphical view of domain structure.
ProDom PD000461; UBQ_conjugat; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00212; UBCc; 1.
SMART graphical view of domain structure.
PROSITE PS00183; UBIQUITIN_CONJUGAT_1; FALSE_NEG.
PS50127; UBIQUITIN_CONJUGAT_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9Y385.
Proteomic databases
PeptideAtlas Q9Y385; -.
Genome annotation databases
Ensembl ENSG00000198833; Homo sapiens. [Contig view]
GeneID 51465; -.
KEGG hsa:51465; -.
Phylogenomic databases
HOVERGEN Q9Y385; -.
Other
ProtoNet Q9Y385.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Endoplasmic reticulum; Ligase; Membrane; Phosphoprotein; Polymorphism; Transmembrane; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   318  318     Ubiquitin-conjugating enzyme E2 J1. PRO_0000082594
TOPO_DOM   1   282  282     Cytoplasmic (Potential). 
TRANSMEM   283   303  21     Anchor for type IV membrane protein (Potential). 
TOPO_DOM   304   318  15     Lumenal (Potential). 
ACT_SITE   91    91        Glycyl thioester intermediate. 
MOD_RES   266   266        Phosphoserine. 
MOD_RES   268   268        Phosphoserine. 
VARIANT   55    55  1     G -> V (in dbSNP:rs8099 [NCBI]). VAR_019689 
VARIANT   229   229  1     L -> V (in dbSNP:rs10502 [NCBI]). VAR_019690 
MUTAGEN   91    91        C->S: Loss of catalytic activity. Slows down degradation of misfolded proteins from the ER. 
CONFLICT   32    33        QP -> HA (in Ref. 3; AAD34071). 
CONFLICT   276   276        Q -> H (in Ref. 2; AAF21505). 
Sequence information
Length: 318 AA [This is the length of the unprocessed precursor] Molecular weight: 35199 Da [This is the MW of the unprocessed precursor] CRC64: CFF5FE00C2BBD39E [This is a checksum on the sequence]
        10         20         30         40         50         60 
METRYNLKSP AVKRLMKEAA ELKDPTDHYH AQPLEDNLFE WHFTVRGPPD SDFDGGVYHG 

        70         80         90        100        110        120 
RIVLPPEYPM KPPSIILLTA NGRFEVGKKI CLSISGHHPE TWQPSWSIRT ALLAIIGFMP 

       130        140        150        160        170        180 
TKGEGAIGSL DYTPEERRAL AKKSQDFCCE GCGSAMKDVL LPLKSGSDSS QADQEAKELA 

       190        200        210        220        230        240 
RQISFKAEVN SSGKTISESD LNHSFSLTDL QDDIPTTFQG ATASTSYGLQ NSSAASFHQP 

       250        260        270        280        290        300 
TQPVAKNTSM SPRQRRAQQQ SQRRLSTSPD VIQGHQPRDN HTDHGGSAVL IVILTLALAA 

       310 
LIFRRIYLAN EYIFDFEL 

Q9Y385 in FASTA format

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