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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
Chapman K.L.,
Newman B.,
Hillaby M.C.,
Freemont A.J.,
Grant M.E.,
Boot-Handford R.,
Wallis G.A.;
"Alpha enolase is upregulated in proliferative chondrocytes in the epiphyseal growth plate and in human osteoarthritic tissue.";
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
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[2]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Crossbred X Angus;
TISSUE=Ileum;
NIH - Mammalian Gene Collection (MGC) project;
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
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[3]
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PROTEIN SEQUENCE OF 270-281 AND 373-394, AND FUNCTION AS AN ENDOTHELIAL HYPOXIC STRESS PROTEIN.
DOI=10.1074/jbc.270.46.27752; PubMed=7499243 [NCBI, ExPASy, EBI, Israel, Japan]
Aaronson R.M.,
Graven K.K.,
Tucci M.,
McDonald R.J.,
Farber H.W.;
"Non-neuronal enolase is an endothelial hypoxic stress protein.";
J. Biol. Chem. 270:27752-27757(1995).
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- FUNCTION: Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons (By similarity).
- CATALYTIC ACTIVITY: 2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
- COFACTOR: Magnesium. Required for catalysis and for stabilizing the dimer (By similarity).
- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
- SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. ENO1 interacts with PLG in the neuronal plasma membrane and promotes its activation. The C-terminal lysine is required for this binding (By similarity).
- SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form (By similarity). ENO1 is localized to the M-band (By similarity).
- TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
- DEVELOPMENTAL STAGE: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
- INDUCTION: Expression increased up to 3-fold by hypoxic stress in vascular endothelial cells.
- SIMILARITY: Belongs to the enolase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 434 AA [This is the length of the unprocessed precursor] |
Molecular weight: 47326 Da [This is the MW of the unprocessed precursor] |
CRC64: 91E2A06F073C5121 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MSILKVHARE IFDSRGNPTV EVDLFTAKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK
70 80 90 100 110 120
GVSKAVEHIN KTIAPALVSK KLNVVEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK
130 140 150 160 170 180
AGAVEKGVPL YRHIADLAGN AEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAENFRE
190 200 210 220 230 240
AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEALELLKNA IGKAGYSDKV
250 260 270 280 290 300
VIGMDVAASE FYRSGKYDLD FKSPDDPSRY ITPDELANLY KSFIRDYPVV SIEDPFDQDD
310 320 330 340 350 360
WEAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVSEKSCNCL LLKVNQIGSV TESLQACKLA
370 380 390 400 410 420
QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTVAPCR SERLAKYNQI LRIEEELGSK
430
AKFAGRSFRN PLAK
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Q9XSJ4 in FASTA format |
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