ID   SEST_DROME              Reviewed;         497 AA.
AC   Q9W1K5; Q9I7V1;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   01-JUL-2008, entry version 57.
DE   Putative sestrin.
GN   ORFNames=CG11299;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA   Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA   Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA   Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA   Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA   Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA   Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA   Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA   Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA   de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA   Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA   Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA   Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA   Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA   Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA   Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA   Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z.,
RA   Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA   Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA   Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA   Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA   Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA   Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA   Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA   Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA   Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA   Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA   Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA   Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA   Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X.,
RA   Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RX   MEDLINE=22426069; PubMed=12537572;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA   Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA   Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a
RT   systematic review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=Berkeley; TISSUE=Embryo;
RX   MEDLINE=22426066; PubMed=12537569;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M.,
RA   George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H.,
RA   Rubin G.M., Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-185 AND SER-190, AND
RP   MASS SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18327897; DOI=10.1021/pr700696a;
RA   Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT   "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL   J. Proteome Res. 7:1675-1682(2008).
CC   -!- INTERACTION:
CC       Q9VXY0:Cyp4s3; NbExp=1; IntAct=EBI-212772, EBI-133844;
CC       O61491-1:Flo; NbExp=1; IntAct=EBI-212772, EBI-212825;
CC       Q9V574:l(2)k10201; NbExp=1; IntAct=EBI-212772, EBI-154227;
CC       Q27889:Pp2B-14D; NbExp=1; IntAct=EBI-212772, EBI-132663;
CC       Q9VTB8:tna; NbExp=1; IntAct=EBI-212772, EBI-162797;
CC   -!- SUBCELLULAR LOCATION: Nucleus (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=Q9W1K5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9W1K5-2; Sequence=VSP_006065, VSP_006066;
CC         Note=No experimental confirmation available;
CC       Name=3;
CC         IsoId=Q9W1K5-3; Sequence=VSP_006067, VSP_006068;
CC         Note=No experimental confirmation available;
CC   -!- SIMILARITY: Belongs to the sestrin family.
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DR   EMBL; AE013599; AAF47053.2; -; Genomic_DNA.
DR   EMBL; AY069626; AAL39771.1; -; mRNA.
DR   RefSeq; NP_001097437.1; -.
DR   RefSeq; NP_001097438.1; -.
DR   RefSeq; NP_611821.1; -.
DR   UniGene; Dm.2037; -.
DR   DIP; DIP:19601N; -.
DR   IntAct; Q9W1K5; -.
DR   Ensembl; CG11299; Drosophila melanogaster.
DR   GeneID; 37755; -.
DR   KEGG; dme:Dmel_CG11299; -.
DR   NMPDR; fig|7227.3.peg.7121; -.
DR   FlyBase; FBgn0034897; CG11299.
DR   HOGENOM; Q9W1K5; -.
DR   BioCyc; DMEL-XXX-02:DMEL-XXX-02-006283-MON; -.
DR   ArrayExpress; Q9W1K5; -.
DR   GermOnline; CG11299; Drosophila melanogaster.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR006730; PA26.
DR   PANTHER; PTHR12474; PA26; 1.
DR   Pfam; PF04636; PA26; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; Nucleus; Phosphoprotein.
FT   CHAIN         1    497       Putative sestrin.
FT                                /FTId=PRO_0000221186.
FT   MOD_RES     185    185       Phosphoserine.
FT   MOD_RES     190    190       Phosphoserine.
FT   VAR_SEQ      80    111       AAARHQCPYLVKRYEKEFINQGGDSAWLGGLD -> SIFRL
FT                                LFPTFISLPNWPKTIAIDWANEEHGLL (in isoform
FT                                2).
FT                                /FTId=VSP_006065.
FT   VAR_SEQ     112    497       Missing (in isoform 2).
FT                                /FTId=VSP_006066.
FT   VAR_SEQ     433    437       LLVRP -> VRNQL (in isoform 3).
FT                                /FTId=VSP_006067.
FT   VAR_SEQ     438    497       Missing (in isoform 3).
FT                                /FTId=VSP_006068.
SQ   SEQUENCE   497 AA;  55853 MW;  39AC03B8F653CA30 CRC64;
     MYYAVDYYAD MGQISQDCFA ATQTGASDFD MDELDDLDQV TQVIGYHPQF HDHFLATQNF
     IMKGDGPLPN DYRYYLAIIA AARHQCPYLV KRYEKEFINQ GGDSAWLGGL DFIPAKLRAI
     YDINKILAHR PWLLRKEHIE RLTKGKNSWS LSEVVHAMVL LSHFHSLSSF VFSCGLTQKL
     DGLSSPKLKS PPAAVAALAP TILITPTSPT EPQKGKPVLA EISLNNANPD YDSQTAASSN
     GGAPPDSANA VADGPDATTL NGYLATAQQL PQQHGISVET LMERMKVLSQ KQDECSEAEL
     SSRFQKVEQQ TAELAAVTPE AAVGVPTNLS HYVDDANFIY QDFARRGTES INTFRIQDYS
     WEDHGYSLVD GLYNDVGIFL DAKFRAAYNL TYCTMGGIKN VDTSKFRRAI WNYIQCIYGI
     RHDDYDYGEV NQLLVRPLKM FIKTACCFPE RITTKDYDSV LVELQDSEKV HVNLMIMEAR
     NQAELLYALR EIMRYMT
//