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UniProtKB/Swiss-Prot entry Q9VJQ4


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MCES_DROME
Primary accession number Q9VJQ4
Secondary accession number Q9N6X4
Integrated into Swiss-Prot on September 5, 2006
Sequence was last modified on October 1, 2002 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 49)
Name and origin of the protein
Protein name mRNA cap guanine-N7 methyltransferase
Synonyms EC 2.1.1.56
mRNA (guanine-N(7)-)-methyltransferase
Gene name
Name: l(2)35Bd
ORFNames: CG3688
From
Drosophila melanogaster (Fruit fly) [TaxID: 7227] 
Taxonomy Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Berkeley;
DOI=10.1126/science.287.5461.2185; PubMed=10731132 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
"The genome sequence of Drosophila melanogaster.";
Science 287:2185-2195(2000).
[2]
GENOME REANNOTATION.
PubMed=12537572 [NCBI, ExPASy, EBI, Israel, Japan]
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review.";
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Berkeley;
TISSUE=Embryo;
PubMed=12537569 [NCBI, ExPASy, EBI, Israel, Japan]
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.;
"A Drosophila full-length cDNA resource.";
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; SER-24; SER-29; SER-46 AND SER-48, AND MASS SPECTROMETRY.
TISSUE=Embryo;
DOI=10.1021/pr700696a; PubMed=18327897 [NCBI, ExPASy, EBI, Israel, Japan]
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
"Phosphoproteome analysis of Drosophila melanogaster embryos.";
J. Proteome Res. 7:1675-1682(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE014134; AAF53430.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY071407; AAL49029.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_523568.2; -.
UniGene Dm.6528
3D structure databases
ModBase Q9VJQ4.
Protein-protein interaction databases
IntAct Q9VJQ4; -.
Organism-specific databases
FlyBase FBgn0001974; l(2)35Bd.
Gene expression databases
ArrayExpress Q9VJQ4; -.
GermOnline CG3688; Drosophila melanogaster.
Ontologies
GO
GO:0005634; Cellular component: nucleus (inferred from sequence or structural similarity from UniProtKB).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0003723; Molecular function: RNA binding (inferred from sequence or structural similarity from UniProtKB).
GO:0006370; Biological process: mRNA capping (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR016899; mRNA_G-N7_MeTrfase.
IPR004971; Pox_MCEL.
Graphical view of domain structure.
Pfam PF03291; Pox_MCEL; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF028762; ABD1; 1.
BLOCKS Q9VJQ4.
Genome annotation databases
Ensembl CG3688; Drosophila melanogaster. [Contig view]
GeneID 34877; -.
KEGG dme:Dmel_CG3688; -.
NMPDR fig|7227.3.peg.2464; -.
Other
ProtoNet Q9VJQ4.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Methyltransferase; mRNA capping; mRNA processing; Nucleus; Phosphoprotein; RNA-binding; S-adenosyl-L-methionine; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   427  427     mRNA cap guanine-N7 methyltransferase. PRO_0000248330
REGION   112   113  2     mRNA cap binding (By similarity). 
BINDING   116   116        S-adenosyl-L-methionine (By similarity). 
BINDING   143   143        S-adenosyl-L-methionine; via carbonyl oxygen (By similarity). 
BINDING   146   146        mRNA cap (By similarity). 
BINDING   152   152        mRNA cap (By similarity). 
BINDING   165   165        S-adenosyl-L-methionine (By similarity). 
BINDING   177   177        mRNA cap (By similarity). 
BINDING   202   202        S-adenosyl-L-methionine (By similarity). 
BINDING   225   225        S-adenosyl-L-methionine; via carbonyl oxygen (By similarity). 
BINDING   229   229        mRNA cap (By similarity). 
BINDING   314   314        mRNA cap (By similarity). 
BINDING   403   403        mRNA cap (By similarity). 
MOD_RES   22    22        Phosphoserine. 
MOD_RES   24    24        Phosphoserine. 
MOD_RES   29    29        Phosphoserine. 
MOD_RES   46    46        Phosphoserine. 
MOD_RES   48    48        Phosphoserine. 
Sequence information
Length: 427 AA [This is the length of the unprocessed precursor] Molecular weight: 48679 Da [This is the MW of the unprocessed precursor] CRC64: E585788C8868F2FB [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSLNYEQNAA DEQFARAHKA VSLSDDEESE GQAETTSAPN QEPHVSKSPR EYYDEPGGKG 

        70         80         90        100        110        120 
NGSGADDQDE PETEAASGAA NTHVVAHHYN ELKEAGRKDR QKSKIFFMRN FNNWIKSQLI 

       130        140        150        160        170        180 
NEYMSQIKQN KRMGDALRVL DMCCGKGGDL LKWEKAAISH LICTDIAEVS VEQCQRRYQD 

       190        200        210        220        230        240 
ILQRSEKSKF ANKFTAEFFA CDSTLVRLRE RYKDPSLQLN LVSCQFAFHY CFESMAQADC 

       250        260        270        280        290        300 
MMRNAAECLK PGGFFIATMP DAYEIIRRLR AAGPDARRFG NDVYSIEFDC ETDPLPLFGA 

       310        320        330        340        350        360 
KYQFHLEGVV DCPEFLVHFP TLVKLGRKYG LQLLKRSTFA DYYKENLHHG RHLLQRMSGL 

       370        380        390        400        410        420 
ESVQPQRCEN DEEFAHVSNF QGAQRSRSVG TLSKSEWEAA TLYLVCAFKK CKNTWDTNGK 


PLFEFDD 

Q9VJQ4 in FASTA format

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