FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
PTM: This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation (Potential).
SIMILARITY: Belongs to the archaeal DNA polymerase II family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ248283; CAB49044.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

E75199; E75199.

RefSeq

NP_125813.1; -.

3D structure databases

ModBase

Q9V2F4.

Enzyme and pathway databases

BioCyc

PABY272844:PAB2404-MON; -.

Ontologies

GO:0008408;	Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887;	Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738;	Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261;	Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00324; -; 1.
PBIL [Tree]

InterPro

IPR003587; Hedgehog_hint_N.
IPR006141; Intein_splicing_site.
IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.

Pfam

PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.

SMART

SM00306; HintN; 1.
SMART graphical view of domain structure.

TIGRFAMs

TIGR01445; intein_Nterm; 1.
TIGR00354; polC; 1.

PROSITE

PS50818; INTEIN_C_TER; 1.
PS50817; INTEIN_N_TER; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9V2F4.

Genome annotation databases

GeneID

1495007; -.

GenomeReviews

AL096836_GR; PYRAB01200.

KEGG

pab:PAB2404; -.

NMPDR

fig|272844.1.peg.131; -.

Phylogenomic databases

HOGENOM

Q9V2F4; -.

Genome annotation databases

CMR

Q9V2F4; PYRAB01200.

Other

ProtoNet

Q9V2F4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Autocatalytic cleavage; Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Protein splicing; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 954`	`954`	`DNA polymerase II large subunit, 1st part (Potential).`	`PRO_0000007304`
`CHAIN`	`955 1139`	`185`	`Pab polC intein (Potential).`	`PRO_0000007305`
`CHAIN`	`1140 1455`	`316`	`DNA polymerase II large subunit, 2nd part (Potential).`	`PRO_0000007306`

Sequence information

Length: 1455 AA [This is the length of the unprocessed precursor]

Molecular weight: 165711 Da [This is the MW of the unprocessed precursor]

CRC64: BB092E7BB2FE0D21 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MELPKEMEEY FEMLQREIDK AYEIAKKARA QGKDPSLDVE IPQATDMAGR VESLVGPPGV 

        70         80         90        100        110        120 
AKRIRELVKE YGKEIAALKI VDEIIEGKFG DLGSREKYAE QAVRTALAIL TEGIVSAPIE 

       130        140        150        160        170        180 
GIANVKIKRN TWADNSEYLA LYYAGPIRSS GGTAQALSVL VGDYVRRKLG LDRFKPSEKH 

       190        200        210        220        230        240 
IERMVEEVDL YHRAVTRLQY HPSPEEVRLA MRNIPIEITG EATDDVEVSH RDVPGVETNQ 

       250        260        270        280        290        300 
LRGGAILVLA EGVLQKAKKL VKYIDKMGIE GWEWLKEFVE AKEKGEPKEE GKEESLAEST 

       310        320        330        340        350        360 
LEETKVEVDM GFYYSLYQKF KEEIAPSDKY AKEVIGGRPL FSDPSKPGGF RLRYGRSRAS 

       370        380        390        400        410        420 
GFATWGINPA TMILVDEFLA IGTQLKTERP GKGAVVTPVT TIEGPIVKLK DGSVLRVDDY 

       430        440        450        460        470        480 
NLALKVREDV EEILYLGDAV IAFGDFVENN QTLLPANYCE EWWILEFVKA LKEIYEVHLE 

       490        500        510        520        530        540 
PFTENEEESI EEASDYLEID PEFLKEMLRD PLRVKPPVEL AIHFSEVLGI PLHPYYTLYW 

       550        560        570        580        590        600 
NSVEPKDVEK LWRLLKNYAE IEWSNFRGIK FAKKIVISQE KLGDSKRTLE LLGLPHTVRD 

       610        620        630        640        650        660 
GNVIVDYPWA AALLTPLGNL NWEFMAKPLY ATIDIINENN EIKLRDRGIS WIGARMGRPE 

       670        680        690        700        710        720 
KAKERKMKPP VQVLFPIGLA GGSSRDIKKA AEEGKVAEVE IAFFKCPKCG HVGPEHLCPN 

       730        740        750        760        770        780 
CGTRKELLWV CPRCNAEYPE SQAEGYNYTC PKCNVKLRPY AKRKIRPSEL LNRAMENVKV 

       790        800        810        820        830        840 
YGVDKLKGVM GMTSGWKMPE PLEKGLLRAK NDVYVFKDGT IRFDATDAPI THFRPREIGV 

       850        860        870        880        890        900 
SVEKLRELGY THDFEGKPLV SEDQIVELKP QDIILSKEAG RYLLKVAKFV DDLLEKFYGL 

       910        920        930        940        950        960 
PRFYNAEKME DLIGHLVIGL APHTSAGIVG RIIGFVDALV GYAHPYFHAA KRRNCFPGDT 

       970        980        990       1000       1010       1020 
RILVQIDGVP QKITLRELYE LFEDERYENM VYVRKKPKRE IKVYSIDLET GKVVLTDIED 

      1030       1040       1050       1060       1070       1080 
VIKAPATDHL IRFELEDGRS FETTVDHPVL VYENGRFIEK RAFEVKEGDK VLVSELELVE 

      1090       1100       1110       1120       1130       1140 
QSSSSQDNPK NENLGSPEHD QLLEIKNIKY VRANDDFVFS LNAKKYHNVI INENIVTHQC 

      1150       1160       1170       1180       1190       1200 
DGDEDAVMLL LDALLNFSRY YLPEKRGGKM DAPLVITTRL DPREVDSEVH NMDIVRYYPL 

      1210       1220       1230       1240       1250       1260 
EFYEATYELK SPKELVGVIE RVEDRLGKPE MYYGLKFTHD TDDIALGPKM SLYKQLGDME 

      1270       1280       1290       1300       1310       1320 
EKVRRQLEVA KRIRAVDEHG VAEKILNSHL IPDLRGNLRS FTRQEFRCVK CNTKFRRPPL 

      1330       1340       1350       1360       1370       1380 
NGKCPVCGGK IVLTVSKGAI EKYLGTAKML VTEYNVKNYT RQRICLTERD IDSLFENVFP 

      1390       1400       1410       1420       1430       1440 
ETQLTLIVNP NDICQRLVMA RTGEVNKSGL LENLSNGSKK TEKAEKAEKP RKKSDEKPKK 

      1450 
KRVISLEEFF SRKSK

Q9V2F4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools