[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), RNA-BINDING, TISSUE SPECIFICITY, AND PHOSPHORYLATION.
TISSUE=Cervix carcinoma;
DOI=10.1016/S0167-4781(00)00065-8; PubMed=11004489 [NCBI, ExPASy, EBI, Israel, Japan]
Sawada Y.,
Miura Y.,
Umeki K.,
Tamaoki T.,
Fujinaga K.,
Ohtaki S.;
"Cloning and characterization of a novel RNA-binding protein SRL300 with RS domains.";
Biochim. Biophys. Acta 1492:191-195(2000).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), PROTEIN SEQUENCE OF 1223-1226 AND 2587-2597, AND FUNCTION.
DOI=10.1017/S1355838200991982; PubMed=10668804 [NCBI, ExPASy, EBI, Israel, Japan]
Blencowe B.J.,
Bauren G.,
Eldridge A.G.,
Issner R.,
Nickerson J.A.,
Rosonina E.,
Sharp P.A.;
"The SRm160/300 splicing coactivator subunits.";
RNA 6:111-120(2000).
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[3]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT THR-804.
TISSUE=Brain;
DOI=10.1093/dnares/4.2.141; PubMed=9205841 [NCBI, ExPASy, EBI, Israel, Japan]
Nagase T.,
Ishikawa K.,
Nakajima D.,
Ohira M.,
Seki N.,
Miyajima N.,
Tanaka A.,
Kotani H.,
Nomura N.,
Ohara O.;
"Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.";
DNA Res. 4:141-150(1997).
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[4]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature03187; PubMed=15616553 [NCBI, ExPASy, EBI, Israel, Japan]
Martin J.,
Han C.,
Gordon L.A.,
Terry A.,
Prabhakar S.,
She X.,
Xie G.,
Hellsten U.,
Chan Y.M.,
Altherr M.,
Couronne O.,
Aerts A.,
Bajorek E.,
Black S.,
Blumer H.,
Branscomb E.,
Brown N.C.,
Bruno W.J.,
Buckingham J.M., ![]()
Callen D.F.,
Campbell C.S.,
Campbell M.L.,
Campbell E.W.,
Caoile C.,
Challacombe J.F.,
Chasteen L.A.,
Chertkov O.,
Chi H.C.,
Christensen M.,
Clark L.M.,
Cohn J.D.,
Denys M.,
Detter J.C.,
Dickson M.,
Dimitrijevic-Bussod M.,
Escobar J.,
Fawcett J.J.,
Flowers D.,
Fotopulos D.,
Glavina T.,
Gomez M.,
Gonzales E.,
Goodstein D.,
Goodwin L.A.,
Grady D.L.,
Grigoriev I.,
Groza M.,
Hammon N.,
Hawkins T.,
Haydu L.,
Hildebrand C.E.,
Huang W.,
Israni S.,
Jett J.,
Jewett P.B.,
Kadner K.,
Kimball H.,
Kobayashi A.,
Krawczyk M.-C.,
Leyba T.,
Longmire J.L.,
Lopez F.,
Lou Y.,
Lowry S.,
Ludeman T.,
Manohar C.F.,
Mark G.A.,
McMurray K.L.,
Meincke L.J.,
Morgan J.,
Moyzis R.K.,
Mundt M.O.,
Munk A.C.,
Nandkeshwar R.D.,
Pitluck S.,
Pollard M.,
Predki P.,
Parson-Quintana B.,
Ramirez L.,
Rash S.,
Retterer J.,
Ricke D.O.,
Robinson D.L.,
Rodriguez A.,
Salamov A.,
Saunders E.H.,
Scott D.,
Shough T.,
Stallings R.L.,
Stalvey M.,
Sutherland R.D.,
Tapia R.,
Tesmer J.G.,
Thayer N.,
Thompson L.S.,
Tice H.,
Torney D.C.,
Tran-Gyamfi M.,
Tsai M.,
Ulanovsky L.E.,
Ustaszewska A.,
Vo N.,
White P.S.,
Williams A.L.,
Wills P.L.,
Wu J.-R.,
Wu K.,
Yang J.,
DeJong P.,
Bruce D.,
Doggett N.A.,
Deaven L.,
Schmutz J.,
Grimwood J.,
Richardson P.,
Rokhsar D.S.,
Eichler E.E.,
Gilna P.,
Lucas S.M.,
Myers R.M.,
Rubin E.M.,
Pennacchio L.A.;
"The sequence and analysis of duplication-rich human chromosome 16.";
Nature 432:988-994(2004).
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[5]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Mural R.J.,
Istrail S.,
Sutton G.G.,
Florea L.,
Halpern A.L.,
Mobarry C.M.,
Lippert R.,
Walenz B.,
Shatkay H.,
Dew I.,
Miller J.R.,
Flanigan M.J.,
Edwards N.J.,
Bolanos R.,
Fasulo D.,
Halldorsson B.V.,
Hannenhalli S.,
Turner R.,
Yooseph S., ![]()
Lu F.,
Nusskern D.R.,
Shue B.C.,
Zheng X.H.,
Zhong F.,
Delcher A.L.,
Huson D.H.,
Kravitz S.A.,
Mouchard L.,
Reinert K.,
Remington K.A.,
Clark A.G.,
Waterman M.S.,
Eichler E.E.,
Adams M.D.,
Hunkapiller M.W.,
Myers E.W.,
Venter J.C.;
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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[6]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-1018 AND 2555-2752.
TISSUE=Blood, Embryonic testis carcinoma, Placenta, Retinoblastoma, and Rhabdomyosarcoma;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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[7]
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PROTEIN SEQUENCE OF 1-11; 44-51; 116-130; 137-155; 357-401; 1001-1013; 1041-1058; 1101-1111; 1119-1128; 1138-1158; 1224-1277; 1316-1352; 1394-1412; 1539-1556; 2104-2113; 2120-2129; 2132-2163; 2181-2221; 2232-2392; 2431-2460 AND 2690-2698, ACETYLATION AT MET-1, AND MASS SPECTROMETRY.
TISSUE=Colon carcinoma;
Bienvenut W.V.,
Heiserich L.,
Boulahbel H.,
Gottlieb E.;
Submitted (JUL-2007) to UniProtKB.
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[8]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 88-185.
TISSUE=Umbilical cord blood;
Ye M.,
Zhang Q.-H.,
Zhou J.,
Shen Y.,
Wu X.-Y.,
Guan Z.Q.,
Wang L.,
Fan H.-Y.,
Mao Y.-F.,
Dai M.,
Huang Q.-H.,
Chen S.-J.,
Chen Z.;
"Human partial CDS from cd34+ stem cells.";
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
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[9]
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IDENTIFICATION IN A PRE-MRNA SPLICING COMPLEX WITH SFRS4; SFRS5; SNRP70; SNRPA1 AND SRRM1.
PubMed=9531537 [NCBI, ExPASy, EBI, Israel, Japan]
Blencowe B.J.,
Issner R.,
Nickerson J.A.,
Sharp P.A.;
"A coactivator of pre-mRNA splicing.";
Genes Dev. 12:996-1009(1998).
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[10]
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IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE SPICEOSOMAL C COMPLEX.
DOI=10.1017/S1355838202021088; PubMed=11991638 [NCBI, ExPASy, EBI, Israel, Japan]
Jurica M.S.,
Licklider L.J.,
Gygi S.P.,
Grigorieff N.,
Moore M.J.;
"Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis.";
RNA 8:426-439(2002).
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[11]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-142; SER-250; THR-252; THR-274; SER-297; SER-351; SER-353; THR-367; SER-424; SER-820; SER-857; THR-866; SER-901; THR-903; SER-908; SER-914; SER-988; SER-994; THR-1003; THR-1043; SER-1069; SER-1073; SER-1079; SER-1083; SER-1103; SER-1124; SER-1132; SER-1179; SER-1214; SER-1219; SER-1320; SER-1326; SER-1397; SER-1404; SER-1421; SER-1423; SER-1441; SER-1443; SER-1444; THR-1453; SER-1483; SER-1499; SER-1501; SER-1502; THR-1511; SER-1517; SER-1519; SER-1522; SER-1559; SER-1579; SER-1581; SER-1582; SER-1600; SER-1601; SER-1618; SER-1621; THR-1880; THR-1962; SER-2044; SER-2046; SER-2071; THR-2092; THR-2104; SER-2272; SER-2335; SER-2382; SER-2398; SER-2407; THR-2409; SER-2426; SER-2449; SER-2581; SER-2692; SER-2694 AND SER-2714, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0404720101; PubMed=15302935 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A.,
Jedrychowski M.,
Schwartz D.,
Elias J.E.,
Villen J.,
Li J.,
Cohn M.A.,
Cantley L.C.,
Gygi S.P.;
"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
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[12]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295; SER-297; SER-924; SER-1382; SER-1387; SER-1497; SER-1501; SER-1502; SER-1600; SER-1601; SER-1616; SER-1620; SER-1621 AND SER-2100, AND MASS SPECTROMETRY.
DOI=10.1021/pr050048h; PubMed=16083285 [NCBI, ExPASy, EBI, Israel, Japan]
Kim J.-E.,
Tannenbaum S.R.,
White F.M.;
"Global phosphoproteome of HT-29 human colon adenocarcinoma cells.";
J. Proteome Res. 4:1339-1346(2005).
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[13]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2675; SER-2677; SER-2684; SER-2688; SER-2702; SER-2706; THR-2738 AND SER-2740, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1016/j.cell.2006.09.026; PubMed=17081983 [NCBI, ExPASy, EBI, Israel, Japan]
Olsen J.V.,
Blagoev B.,
Gnad F.,
Macek B.,
Kumar C.,
Mortensen P.,
Mann M.;
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.";
Cell 127:635-648(2006).
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[14]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-367; SER-778; SER-780; SER-783; SER-871; SER-876; SER-952; SER-954; SER-1072; SER-1073; SER-1878; THR-1880; SER-2032; THR-2034; SER-2044; SER-2046; SER-2272; THR-2289; THR-2291; THR-2316; SER-2382; SER-2449; SER-2692 AND SER-2694, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1038/nbt1240; PubMed=16964243 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A.,
Villen J.,
Gerber S.A.,
Rush J.,
Gygi S.P.;
"A probability-based approach for high-throughput protein phosphorylation analysis and site localization.";
Nat. Biotechnol. 24:1285-1292(2006).
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[15]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1124; SER-1188; SER-1329; SER-2032; SER-2044; SER-2067; SER-2272; SER-2692 AND SER-2694, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0507066103; PubMed=16565220 [NCBI, ExPASy, EBI, Israel, Japan]
Nousiainen M.,
Sillje H.H.W.,
Sauer G.,
Nigg E.A.,
Koerner R.;
"Phosphoproteome analysis of the human mitotic spindle.";
Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).
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[16]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1531 AND SER-1975, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1021/pr070152u; PubMed=17924679 [NCBI, ExPASy, EBI, Israel, Japan]
Yu L.-R.,
Zhu Z.,
Chan K.C.,
Issaq H.J.,
Dimitrov D.S.,
Veenstra T.D.;
"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.";
J. Proteome Res. 6:4150-4162(2007).
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[17]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295; SER-297; SER-323; SER-377; THR-383; THR-384; SER-387; SER-395; SER-398; THR-400; SER-415; SER-418; THR-428; SER-435; SER-440; SER-755; SER-759; SER-761; SER-763; SER-764; SER-774; SER-778; SER-780; SER-783; SER-883; THR-885; SER-890; SER-894; SER-895; SER-950; SER-952; SER-954; THR-983; SER-988; SER-994; TYR-996; THR-1063; SER-1069; THR-1071; SER-1072; SER-1073; SER-1099; SER-1101; SER-1103; THR-1106; SER-1110; SER-1112; SER-1124; SER-1129; THR-1205; THR-1208; SER-1218; SER-1219; SER-1320; SER-1329; SER-1397; SER-1398; SER-1403; SER-1404; THR-1413; SER-1415; THR-1492; SER-1497; SER-1499; SER-1501; SER-1502; THR-1571; SER-1579; SER-1581; SER-1582; SER-1592; SER-1596; SER-1598; SER-1600; SER-1601; SER-1616; SER-1618; SER-1620; SER-1621; SER-1636; SER-1638; SER-1640; SER-1641; SER-1657; SER-1658; SER-1682; THR-1684; SER-1690; SER-1691; SER-1693; SER-1694; SER-1876; SER-1878; THR-1880; SER-1958; SER-1960; THR-1962; SER-2018; SER-2020; THR-2022; SER-2030; SER-2032; THR-2034; SER-2042; SER-2044; SER-2046; SER-2067; THR-2069; SER-2071; SER-2272; SER-2407 AND THR-2409, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0611217104; PubMed=17287340 [NCBI, ExPASy, EBI, Israel, Japan]
Molina H.,
Horn D.M.,
Tang N.,
Mathivanan S.,
Pandey A.;
"Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry.";
Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).
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[18]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-377; SER-387; SER-398; SER-2436 AND SER-2453, AND MASS SPECTROMETRY.
DOI=10.1126/science.1140321; PubMed=17525332 [NCBI, ExPASy, EBI, Israel, Japan]
Matsuoka S.,
Ballif B.A.,
Smogorzewska A.,
McDonald E.R. III,
Hurov K.E.,
Luo J.,
Bakalarski C.E.,
Zhao Z.,
Solimini N.,
Lerenthal Y.,
Shiloh Y.,
Gygi S.P.,
Elledge S.J.;
"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.";
Science 316:1160-1166(2007).
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[19]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295; SER-297; SER-875; SER-954; THR-983; SER-988; THR-1003; SER-1014; THR-1043; THR-1106; SER-1112; SER-1320; SER-1419; SER-1878; THR-1880; SER-2046; SER-2100; SER-2132; THR-2397; SER-2398; SER-2581 AND SER-2694, AND MASS SPECTROMETRY.
DOI=10.2116/analsci.24.161; PubMed=18187866 [NCBI, ExPASy, EBI, Israel, Japan]
Imami K.,
Sugiyama N.,
Kyono Y.,
Tomita M.,
Ishihama Y.;
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.";
Anal. Sci. 24:161-166(2008).
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[20]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295; SER-297; SER-322; SER-323; SER-1404; THR-1413; SER-2272; SER-2398; SER-2407; THR-2409; SER-2688 AND SER-2694, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T.,
Yi W.,
Lu B.,
Park S.K.,
Xu T.,
Lee J.-D.,
Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
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- FUNCTION: Part of pre- and post-splicing multiprotein mRNP complexes. May be involved in pre-mRNA processing events. Binds to RNA.
- SUBUNIT: Component of the active spliceosome. Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRP70, SNRPA1, SRRM1 and SRRM2. Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8.
- INTERACTION:
Q16611:BAK1; NbExp=1; IntAct=EBI-1050142, EBI-519866;
Q15287:RNPS1; NbExp=1; IntAct=EBI-1050142, EBI-395959;
P31946:YWHAB; NbExp=1; IntAct=EBI-1050142, EBI-359815;
P61981:YWHAG; NbExp=1; IntAct=EBI-1050142, EBI-359832;
- SUBCELLULAR LOCATION: Nucleus speckle.
- ALTERNATIVE PRODUCTS:
3 named isoforms [FASTA] produced by alternative splicing.
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| Name | 2 |
| Isoform ID | Q9UQ35-2 |
| Note: No experimental confirmation available. |
| Features which should be applied to build the isoform sequence: VSP_020187. |
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- TISSUE SPECIFICITY: Expressed in liver, placenta, and white blood cells.
- PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
- SIMILARITY: Belongs to the CWC21 family.
- SEQUENCE CAUTION:
- Sequence=AAF21439.1; Type=Frameshift; Positions=Several;
- Sequence=AAF28898.1; Type=Frameshift; Positions=167, 175, 179;
- Sequence=AAH70050.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence
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