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UniProtKB/Swiss-Prot entry Q9UBF8


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PI4KB_HUMAN
Primary accession number Q9UBF8
Secondary accession numbers O15096 P78405 Q5VWC1 Q9BWR6
Integrated into Swiss-Prot on November 14, 2003
Sequence was last modified on May 1, 2000 (Sequence version 1)
Annotations were last modified on    June 10, 2008 (Entry version 60)
Name and origin of the protein
Protein name Phosphatidylinositol 4-kinase beta
Synonyms EC 2.7.1.67
PtdIns 4-kinase beta
PI4K-beta
PI4Kbeta
NPIK
PI4K92
Gene name
Name: PI4KB
Synonyms: PIK4CB
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
DOI=10.1093/dnares/4.4.273; PubMed=9405935 [NCBI, ExPASy, EBI, Israel, Japan]
Suzuki K., Hirano H., Okutomi K., Suzuki M., Kuga Y., Fujiwara T., Kanemoto N., Isono K., Horie M.;
"Identification and characterization of a novel human phosphatidylinositol 4-kinase.";
DNA Res. 4:273-280(1997).
[2]
NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
TISSUE=Brain;
DOI=10.1093/dnares/4.4.301; PubMed=9405938 [NCBI, ExPASy, EBI, Israel, Japan]
Saito T., Seki N., Ishii H., Ohira M., Hayashi A., Kozuma S., Hori T.-A.;
"Complementary DNA cloning and chromosomal mapping of a novel phosphatidylinositol kinase gene.";
DNA Res. 4:301-305(1997).
[3]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ENZYME REGULATION, AND TISSUE SPECIFICITY.
TISSUE=Heart, and Placenta;
DOI=10.1074/jbc.272.7.4384; PubMed=9020160 [NCBI, ExPASy, EBI, Israel, Japan]
Meyers R., Cantley L.C.;
"Cloning and characterization of a wortmannin-sensitive human phosphatidylinositol 4-kinase.";
J. Biol. Chem. 272:4384-4390(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04727; PubMed=16710414 [NCBI, ExPASy, EBI, Israel, Japan]
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
"The DNA sequence and biological annotation of human chromosome 1.";
Nature 441:315-321(2006).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
TISSUE=Brain, and Testis;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
SUBCELLULAR LOCATION.
DOI=10.1074/jbc.272.20.13236; PubMed=9148941 [NCBI, ExPASy, EBI, Israel, Japan]
Wong K., Meyers R., Cantley L.C.;
"Subcellular locations of phosphatidylinositol 4-kinase isoforms.";
J. Biol. Chem. 272:13236-13241(1997).
[7]
FUNCTION.
DOI=10.1038/12993; PubMed=10559940 [NCBI, ExPASy, EBI, Israel, Japan]
Godi A., Pertile P., Meyers R., Marra P., Di Tullio G., Iurisci C., Luini A., Corda D., De Matteis M.A.;
"ARF mediates recruitment of PtdIns-4-OH kinase-beta and stimulates synthesis of PtdIns(4,5)P2 on the Golgi complex.";
Nat. Cell Biol. 1:280-287(1999).
[8]
PHOSPHORYLATION.
PubMed=11277933 [NCBI, ExPASy, EBI, Israel, Japan]
Suer S., Sickmann A., Meyer H.E., Herberg F.W., Heilmeyer L.M.G. Jr.;
"Human phosphatidylinositol 4-kinase isoform PI4K92. Expression of the recombinant enzyme and determination of multiple phosphorylation sites.";
Eur. J. Biochem. 268:2099-2106(2001).
[9]
FUNCTION.
DOI=10.1080/1521654031000090896; PubMed=12749687 [NCBI, ExPASy, EBI, Israel, Japan]
Heilmeyer L.M.G. Jr., Vereb G. Jr., Vereb G., Kakuk A., Szivak I.;
"Mammalian phosphatidylinositol 4-kinases.";
IUBMB Life 55:59-65(2003).
[10]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-428 AND SER-511, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1016/j.cell.2006.09.026; PubMed=17081983 [NCBI, ExPASy, EBI, Israel, Japan]
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.";
Cell 127:635-648(2006).
[11]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-277, AND MASS SPECTROMETRY.
DOI=10.2116/analsci.24.161; PubMed=18187866 [NCBI, ExPASy, EBI, Israel, Japan]
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.;
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.";
Anal. Sci. 24:161-166(2008).
[12]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-266; SER-511 AND THR-517, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ011121; CAA09495.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ011122; -; NOT_ANNOTATED_CDS; mRNA.[EMBL / GenBank / DDBJ]
AJ011123; CAA09496.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB005910; BAA21661.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U81802; AAC51156.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL391069; CAH70324.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC000029; AAH00029.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC040300; AAH40300.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JC5706; JC5706.
RefSeq NP_002642.1; -.
UniGene Hs.632465
3D structure databases
HSSP P48736; 1E8Y. [HSSP ENTRY / PDB]
ModBase Q9UBF8.
Protein-protein interaction databases
IntAct Q9UBF8; -.
PTM databases
PhosphoSite Q9UBF8; -.
Organism-specific databases
H-InvDB HIX0001056; -.
HGNC HGNC:8984; PI4KB.
GeneLynx PI4KB; Homo sapiens.
GenAtlas PI4KB.
HPA HPA006280; -.
HPA006281; -.
MIM 602758; gene. [NCBI / EBI]
PharmGKB PA33317; -.
GeneCards Q9UBF8.
Gene expression databases
ArrayExpress Q9UBF8; -.
CleanEx HS_PI4KB; -.
GermOnline ENSG00000143393; Homo sapiens.
Ontologies
GO
GO:0005768; Cellular component: endosome (traceable author statement from ProtInc).
GO:0004430; Molecular function: 1-phosphatidylinositol 4-kinase activity (traceable author statement from ProtInc).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006661; Biological process: phosphatidylinositol biosynthetic process (traceable author statement from ProtInc).
GO:0006898; Biological process: receptor-mediated endocytosis (traceable author statement from ProtInc).
GO:0007165; Biological process: signal transduction (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR000403; PI3/4_kinase_cat.
IPR015433; PI_Kinase.
Graphical view of domain structure.
Gene3D G3DSA:1.10.1070.11; PI3/4_kinase_cat; 1.
PANTHER PTHR10048; PI_Kinase; 1.
Pfam PF00454; PI3_PI4_kinase; 1.
Pfam graphical view of domain structure.
SMART SM00146; PI3Kc; 1.
SMART graphical view of domain structure.
PROSITE PS00915; PI3_4_KINASE_1; 1.
PS00916; PI3_4_KINASE_2; 1.
PS50290; PI3_4_KINASE_3; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9UBF8.
Genome annotation databases
Ensembl ENSG00000143393; Homo sapiens. [Contig view]
GeneID 5298; -.
KEGG hsa:5298; -.
Phylogenomic databases
HOVERGEN Q9UBF8; -.
Other
SOURCE PI4KB; Homo sapiens.
ProtoNet Q9UBF8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Endoplasmic reticulum; Golgi apparatus; Kinase; Membrane; Mitochondrion; Phosphoprotein; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   816  816     Phosphatidylinositol 4-kinase beta. PRO_0000088829
DOMAIN   558   765  208     PI3K/PI4K. 
MOD_RES   258   258        Phosphoserine. 
MOD_RES   263   263        Phosphothreonine. 
MOD_RES   266   266        Phosphoserine. 
MOD_RES   277   277        Phosphoserine. 
MOD_RES   294   294        Phosphoserine. 
MOD_RES   428   428        Phosphoserine. 
MOD_RES   438   438        Phosphothreonine. 
MOD_RES   511   511        Phosphoserine. 
MOD_RES   517   517        Phosphothreonine. 
MOD_RES   519   519        Phosphothreonine. 
VAR_SEQ   304   318        Missing (in isoform 2). VSP_050627
CONFLICT   216   216        L -> V (in Ref. 3; AAC51156). 
CONFLICT   339   340        LA -> VV (in Ref. 3; AAC51156). 
CONFLICT   370   370        P -> S (in Ref. 3; AAC51156). 
Sequence information
Length: 816 AA [This is the length of the unprocessed precursor] Molecular weight: 91379 Da [This is the MW of the unprocessed precursor] CRC64: CAD8791729BB4308 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGDTVVEPAP LKPTSEPTSG PPGNNGGSLL SVITEGVGEL SVIDPEVAQK ACQEVLEKVK 

        70         80         90        100        110        120 
LLHGGVAVSS RGTPLELVNG DGVDSEIRCL DDPPAQIREE EDEMGAAVAS GTAKGARRRR 

       130        140        150        160        170        180 
QNNSAKQSWL LRLFESKLFD ISMAISYLYN SKEPGVQAYI GNRLFCFRNE DVDFYLPQLL 

       190        200        210        220        230        240 
NMYIHMDEDV GDAIKPYIVH RCRQSINFSL QCALLLGAYS SDMHISTQRH SRGTKLRKLI 

       250        260        270        280        290        300 
LSDELKPAHR KRELPSLSPA PDTGLSPSKR THQRSKSDAT ASISLSSNLK RTASNPKVEN 

       310        320        330        340        350        360 
EDEELSSSTE SIDNSFSSPV RLAPEREFIK SLMAIGKRLA TLPTKEQKTQ RLISELSLLN 

       370        380        390        400        410        420 
HKLPARVWLP TAGFDHHVVR VPHTQAVVLN SKDKAPYLIY VEVLECENFD TTSVPARIPE 

       430        440        450        460        470        480 
NRIRSTRSVE NLPECGITHE QRAGSFSTVP NYDNDDEAWS VDDIGELQVE LPEVHTNSCD 

       490        500        510        520        530        540 
NISQFSVDSI TSQESKEPVF IAAGDIRRRL SEQLAHTPTA FKRDPEDPSA VALKEPWQEK 

       550        560        570        580        590        600 
VRRIREGSPY GHLPNWRLLS VIVKCGDDLR QELLAFQVLK QLQSIWEQER VPLWIKPYKI 

       610        620        630        640        650        660 
LVISADSGMI EPVVNAVSIH QVKKQSQLSL LDYFLQEHGS YTTEAFLSAQ RNFVQSCAGY 

       670        680        690        700        710        720 
CLVCYLLQVK DRHNGNILLD AEGHIIHIDF GFILSSSPRN LGFETSAFKL TTEFVDVMGG 

       730        740        750        760        770        780 
LDGDMFNYYK MLMLQGLIAA RKHMDKVVQI VEIMQQGSQL PCFHGSSTIR NLKERFHMSM 

       790        800        810 
TEEQLQLLVE QMVDGSMRSI TTKLYDGFQY LTNGIM 

Q9UBF8 in FASTA format

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