ID   GID2_ARATH              Reviewed;         151 AA.
AC   Q9STX3; Q8LAQ7;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   22-JUL-2008, entry version 47.
DE   RecName: Full=F-box protein GID2;
DE   AltName: Full=Protein SLEEPY 1;
GN   Name=GID2; Synonyms=SLY1; OrderedLocusNames=At4g24210;
GN   ORFNames=T22A6.40;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083488; PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   MEDLINE=22610498; PubMed=12724538; DOI=10.1105/tpc.010827;
RA   McGinnis K.M., Thomas S.G., Soule J.D., Strader L.C., Zale J.M.,
RA   Sun T.-P., Steber C.M.;
RT   "The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an
RT   SCF E3 ubiquitin ligase.";
RL   Plant Cell 15:1120-1130(2003).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, COMPONENT OF A SCF COMPLEX,
RP   INTERACTION WITH GAI AND RGA, AND MUTAGENESIS OF GLU-138.
RX   PubMed=15155881; DOI=10.1105/tpc.020958;
RA   Dill A., Thomas S.G., Hu J., Steber C.M., Sun T.-P.;
RT   "The Arabidopsis F-box protein SLEEPY1 targets gibberellin signaling
RT   repressors for gibberellin-induced degradation.";
RL   Plant Cell 16:1392-1405(2004).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, COMPONENT OF A SCF COMPLEX WITH SKP1B
RP   AND CUL1, INTERACTION WITH SKP1A; SKP1B; GAI AND RGA, AND MUTAGENESIS
RP   OF GLU-138.
RX   PubMed=15161962; DOI=10.1105/tpc.021386;
RA   Fu X., Richards D.E., Fleck B., Xie D., Burton N., Harberd N.P.;
RT   "The Arabidopsis mutant sleepy1gar2-1 protein promotes plant growth by
RT   increasing the affinity of the SCFSLY1 E3 ubiquitin ligase for DELLA
RT   protein substrates.";
RL   Plant Cell 16:1406-1418(2004).
RN   [7]
RP   INTERACTION WITH GAI; RGA; RGL1 AND RGL3.
RX   PubMed=15173565; DOI=10.1104/pp.104.039578;
RA   Tyler L., Thomas S.G., Hu J., Dill A., Alonso J.M., Ecker J.R.,
RA   Sun T.-P.;
RT   "Della proteins and gibberellin-regulated seed germination and floral
RT   development in Arabidopsis.";
RL   Plant Physiol. 135:1008-1019(2004).
RN   [8]
RP   FUNCTION.
RX   PubMed=15308775; DOI=10.1073/pnas.0404287101;
RA   Strader L.C., Ritchie S., Soule J.D., McGinnis K.M., Steber C.M.;
RT   "Recessive-interfering mutations in the gibberellin signaling gene
RT   SLEEPY1 are rescued by overexpression of its homologue, SNEEZY.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:12771-12776(2004).
CC   -!- FUNCTION: Essential component of the SCF-type E3 ligase complex,
CC       SCF(GID2), a complex that positively regulates the gibberellin
CC       signaling pathway. Upon gibberellin treatment, the SCF(GID2)
CC       complex mediates the ubiquitination and subsequent degradation of
CC       DELLA proteins (GAI, RGA and RGL2), some repressors of the
CC       gibberellin pathway, leading to activate the pathway.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Part of some SCF(GID2) complex, which consist of SKP1B,
CC       CUL1 cullin, GID2/SLY1 and some RING box protein. Interacts
CC       directly with SKP1A and SKP1B. Interacts directly with DELLA
CC       proteins GAI, RGA, RGL1, RGL3 and probably RGL2. May have a higher
CC       affinity for phosphorylated DELLA proteins.
CC   -!- INTERACTION:
CC       Q94AH6:CUL1; NbExp=1; IntAct=EBI-619033, EBI-532411;
CC       Q9LQT8:GAI; NbExp=3; IntAct=EBI-619033, EBI-963606;
CC       Q9SLH3:RGA; NbExp=2; IntAct=EBI-619033, EBI-963624;
CC       Q9FHW7:SKP1B; NbExp=1; IntAct=EBI-619033, EBI-604076;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Expressed in all tissues tested, including
CC       rosette leaves, green siliques, flowers, stems, cauline leaves and
CC       seedlings.
CC   -!- SIMILARITY: Contains 1 F-box domain.
CC   -!- WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database
CC       for the ubiquitin/26S proteasome proteolytic pathway in plants;
CC       URL="http://plantsubq.genomics.purdue.edu/";
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DR   EMBL; AL078637; CAB45056.1; -; Genomic_DNA.
DR   EMBL; AL161561; CAB79331.1; -; Genomic_DNA.
DR   EMBL; BT004192; AAO42210.1; -; mRNA.
DR   EMBL; BT005395; AAO63815.1; -; mRNA.
DR   EMBL; AY087672; AAM65209.1; -; mRNA.
DR   PIR; T09884; T09884.
DR   RefSeq; NP_194152.1; -.
DR   UniGene; At.43332; -.
DR   IntAct; Q9STX3; -.
DR   GeneID; 828522; -.
DR   GenomeReviews; CT486007_GR; AT4G24210.
DR   KEGG; ath:AT4G24210; -.
DR   NMPDR; fig|3702.1.peg.20308; -.
DR   TAIR; At4g24210; -.
DR   GermOnline; AT4G24210; Arabidopsis thaliana.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR001810; F-box.
DR   Pfam; PF00646; F-box; 1.
DR   SMART; SM00256; FBOX; 1.
DR   PROSITE; PS50181; FBOX; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Gibberellin signaling pathway; Nucleus;
KW   Ubl conjugation pathway.
FT   CHAIN         1    151       F-box protein GID2.
FT                                /FTId=PRO_0000119962.
FT   DOMAIN       29     75       F-box.
FT   MUTAGEN     138    138       E->K: In gar2-1/sly1-d; gain of function
FT                                allele that promotes plant growth by
FT                                increasing the affinity with DELLA
FT                                protein substrates.
FT   CONFLICT      3      3       R -> L (in Ref. 3; AAM65209).
SQ   SEQUENCE   151 AA;  17455 MW;  4404DE81E9DFDA9D CRC64;
     MKRSTTDSDL AGDAHNETNK KMKSTEEEEI GFSNLDENLV YEVLKHVDAK TLAMSSCVSK
     IWHKTAQDER LWELICTRHW TNIGCGQNQL RSVVLALGGF RRLHSLYLWP LSKPNPRARF
     GKDELKLTLS LLSIRYYEKM SFTKRPLPES K
//