ID   GIGAN_ARATH             Reviewed;        1173 AA.
AC   Q9SQI2; O64381; O80545; Q9S7N0;
DT   05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT   05-DEC-2001, sequence version 2.
DT   22-JUL-2008, entry version 51.
DE   RecName: Full=Protein GIGANTEA;
GN   Name=GI; OrderedLocusNames=At1g22770; ORFNames=T22J18.6;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=99400613; PubMed=10469647; DOI=10.1093/emboj/18.17.4679;
RA   Fowler S., Lee K., Onouchi H., Samach A., Richardson K., Morris B.,
RA   Coupland G., Putterill J.;
RT   "GIGANTEA: a circadian clock-controlled gene that regulates
RT   photoperiodic flowering in Arabidopsis and encodes a protein with
RT   several possible membrane-spanning domains.";
RL   EMBO J. 18:4679-4688(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia; TISSUE=Leaf, and Stem;
RX   MEDLINE=99407029; PubMed=10477524; DOI=10.1126/science.285.5433.1579;
RA   Park D.H., Somers D.E., Kim Y.S., Choy Y.H., Lim H.K., Soh M.S.,
RA   Kim H.J., Kay S.A., Nam H.G.;
RT   "Control of circadian rhythms and photoperiodic flowering by the
RT   Arabidopsis GIGANTEA gene.";
RL   Science 285:1579-1582(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=98034136; PubMed=9369203; DOI=10.1016/S0014-5793(97)01190-3;
RA   Terryn N., Neyt P., de Clercq R., de Keyser A., van den Daele H.,
RA   Ardiles W., Dehais P., Rouze P., Gielen J., Villarroel R.,
RA   van Montagu M.;
RT   "Sequence analysis of a 24-kb contiguous genomic region at the
RT   Arabidopsis thaliana PFL locus on chromosome 1.";
RL   FEBS Lett. 416:156-160(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   MEDLINE=20402613; PubMed=10920210; DOI=10.1073/pnas.170283997;
RA   Huq E., Tepperman J.M., Quail P.H.;
RT   "GIGANTEA is a nuclear protein involved in phytochrome signaling in
RT   Arabidopsis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:9789-9794(2000).
RN   [6]
RP   INTERACTION WITH SPY.
RX   PubMed=15155885; DOI=10.1105/tpc.019224;
RA   Tseng T.-S., Salome P.A., McClung C.R., Olszewski N.E.;
RT   "SPINDLY and GIGANTEA interact and act in Arabidopsis thaliana
RT   pathways involved in light responses, flowering, and rhythms in
RT   cotyledon movements.";
RL   Plant Cell 16:1550-1563(2004).
CC   -!- FUNCTION: Involved in regulation of circadian rhythm and
CC       photoperiodic flowering. May play a role in maintenance of
CC       circadian amplitude and period length. Is involved in phytochrome
CC       B signaling. Regulates 'CONSTANS' in the long-day flowering
CC       pathway.
CC   -!- SUBUNIT: Interacts with SPY.
CC   -!- INTERACTION:
CC       Q94BT6:ADO1; NbExp=4; IntAct=EBI-446380, EBI-300691;
CC       Q8W420:ADO2; NbExp=2; IntAct=EBI-446380, EBI-1015688;
CC       Q9C9W9:ADO3; NbExp=10; IntAct=EBI-446380, EBI-401228;
CC       Q8W1E3:DOF5.5; NbExp=2; IntAct=EBI-446380, EBI-1536051;
CC       Q96301:SPY; NbExp=1; IntAct=EBI-446380, EBI-446372;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Widely expressed with highest levels in
CC       inflorescence apices, young flowers and young siliques.
CC   -!- DEVELOPMENTAL STAGE: Found at all stages.
CC   -!- INDUCTION: Expressed with a circadian rhythm with the highest
CC       level 10 hours into the light and the lowest level at dawn. The
CC       peak of expression in long days is slightly lower, shifted later
CC       and the decrease is slower.
CC   -!- SIMILARITY: Belongs to the GIGANTEA family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC25507.1; Type=Erroneous gene model prediction;
CC       Sequence=AAF00023.1; Type=Erroneous gene model prediction;
CC       Sequence=CAA72908.1; Type=Erroneous gene model prediction;
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DR   EMBL; AJ133786; CAB56039.1; -; mRNA.
DR   EMBL; AF105064; AAF00092.1; -; mRNA.
DR   EMBL; AF076686; AAF00023.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; Y12227; CAA72908.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AC003979; AAC25507.1; ALT_SEQ; Genomic_DNA.
DR   PIR; T52575; T52575.
DR   RefSeq; NP_564180.1; -.
DR   UniGene; At.209; -.
DR   IntAct; Q9SQI2; -.
DR   GeneID; 838883; -.
DR   GenomeReviews; CT485782_GR; AT1G22770.
DR   KEGG; ath:AT1G22770; -.
DR   NMPDR; fig|3702.1.peg.2647; -.
DR   TAIR; At1g22770; -.
DR   ArrayExpress; Q9SQI2; -.
DR   GermOnline; AT1G22770; Arabidopsis thaliana.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
PE   1: Evidence at protein level;
KW   Biological rhythms; Complete proteome; Developmental protein;
KW   Differentiation; Flowering; Nucleus; Phytochrome signaling pathway.
FT   CHAIN         1   1173       Protein GIGANTEA.
FT                                /FTId=PRO_0000087489.
SQ   SEQUENCE   1173 AA;  127876 MW;  71CFDE7D39E73549 CRC64;
     MASSSSSERW IDGLQFSSLL WPPPRDPQQH KDQVVAYVEY FGQFTSEQFP DDIAELVRHQ
     YPSTEKRLLD DVLAMFVLHH PEHGHAVILP IISCLIDGSL VYSKEAHPFA SFISLVCPSS
     ENDYSEQWAL ACGEILRILT HYNRPIYKTE QQNGDTERNC LSKATTSGSP TSEPKAGSPT
     QHERKPLRPL SPWISDILLA APLGIRSDYF RWCSGVMGKY AAGELKPPTI ASRGSGKHPQ
     LMPSTPRWAV ANGAGVILSV CDDEVARYET ATLTAVAVPA LLLPPPTTSL DEHLVAGLPA
     LEPYARLFHR YYAIATPSAT QRLLLGLLEA PPSWAPDALD AAVQLVELLR AAEDYASGVR
     LPRNWMHLHF LRAIGIAMSM RAGVAADAAA ALLFRILSQP ALLFPPLSQV EGVEIQHAPI
     GGYSSNYRKQ IEVPAAEATI EATAQGIASM LCAHGPEVEW RICTIWEAAY GLIPLNSSAV
     DLPEIIVATP LQPPILSWNL YIPLLKVLEY LPRGSPSEAC LMKIFVATVE TILSRTFPPE
     SSRELTRKAR SSFTTRSATK NLAMSELRAM VHALFLESCA GVELASRLLF VVLTVCVSHE
     AQSSGSKRPR SEYASTTENI EANQPVSNNQ TANRKSRNVK GQGPVAAFDS YVLAAVCALA
     CEVQLYPMIS GGGNFSNSAV AGTITKPVKI NGSSKEYGAG IDSAISHTRR ILAILEALFS
     LKPSSVGTPW SYSSSEIVAA AMVAAHISEL FRRSKALTHA LSGLMRCKWD KEIHKRASSL
     YNLIDVHSKV VASIVDKAEP LEAYLKNTPV QKDSVTCLNW KQENTCASTT CFDTAVTSAS
     RTEMNPRGNH KYARHSDEGS GRPSEKGIKD FLLDASDLAN FLTADRLAGF YCGTQKLLRS
     VLAEKPELSF SVVSLLWHKL IAAPEIQPTA ESTSAQQGWR QVVDALCNVV SATPAKAAAA
     VVLQAERELQ PWIAKDDEEG QKMWKINQRI VKVLVELMRN HDRPESLVIL ASASDLLLRA
     TDGMLVDGEA CTLPQLELLE ATARAIQPVL AWGPSGLAVV DGLSNLLKCR LPATIRCLSH
     PSAHVRALST SVLRDIMNQS SIPIKVTPKL PTTEKNGMNS PSYRFFNAAS IDWKADIQNC
     LNWEAHSLLS TTMPTQFLDT AARELGCTIS LSQ
//