[1]	NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE. Juergensen K., Buck F., Lange S., Kleine M., Grundler F.M.W.; "A Beta vulgaris cDNA coding for a putative cytoplasmatic malate dehydrogenase."; (er) Plant Gene Register PGR00-023.

Comments

CATALYTIC ACTIVITY: (S)-malate + NAD⁺ = oxaloacetate + NADH.
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 2 family.

Copyright

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Cross-references

Sequence databases

EMBL

AJ251083; CAB61618.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P11708; 4MDH. [HSSP ENTRY / PDB]

ModBase

Q9SML8.

Family and domain databases

InterPro

IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR011274; Malate_DHase_NAD-dep_euk.
IPR010945; Malate_DHase_SF1.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR23382; MDH_SF1; 1.

Pfam

PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000102; Lac_mal_DH; 1.

ProDom

PD003052; Mal_dehydrog; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR01759; MalateDH-SF1; 1.
TIGR01758; MDH_euk_cyt; 1.

PS00068; MDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 332`	`332`	`Malate dehydrogenase, cytoplasmic.`	`PRO_0000113414`
`NP_BIND`	`12 18`	`7`	`NAD (By similarity).`
`NP_BIND`	`130 132`	`3`	`NAD (By similarity).`
`ACT_SITE`	`188 188`		`Proton acceptor (By similarity).`
`BINDING`	`93 93`		`Substrate (By similarity).`
`BINDING`	`99 99`		`Substrate (By similarity).`
`BINDING`	`106 106`		`NAD (By similarity).`
`BINDING`	`113 113`		`NAD (By similarity).`
`BINDING`	`132 132`		`Substrate (By similarity).`
`BINDING`	`163 163`		`Substrate (By similarity).`

Sequence information

Length: 332 AA [This is the length of the unprocessed precursor]

Molecular weight: 35434 Da [This is the MW of the unprocessed precursor]

CRC64: 2B9E6495EF0CD12A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAVEPLRVLV TGAAGQIGYA LVPMIARGVM LGANQPVILH MLDIPPAAEA LNGVKMELVD 

        70         80         90        100        110        120 
AAFPLLKGVV ATTDVAEACK GVNVAVMVGG FPRKEGMERK DVMPKNVSIY KSQASALEQY 

       130        140        150        160        170        180 
AAPNCKVLVV ANPANTNALI LKEFAPSIPE KNITCLTRLD HNRALGQISE RLNAQVSDVK 

       190        200        210        220        230        240 
NVIIWGNHSS SQYPDVNPCT VKTGSGEKAV RELVADDAWL NGEFITTVQQ RGAAIIKARK 

       250        260        270        280        290        300 
LSSALSAASS ACDHIRDWVL GTPEGTWVSM GVYSDGSYNV PAGIIYSFPV TCKDGEWKIV 

       310        320        330 
QGLPIDEVSR QKMDATGAEL VEEKALAYSC LT

Q9SML8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools