ID   EBF1_ARATH              Reviewed;         628 AA.
AC   Q9SKK0; Q0WM37;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   22-JUL-2008, entry version 57.
DE   RecName: Full=EIN3-binding F-box protein 1;
DE   AltName: Full=F-box/LRR-repeat protein 6;
GN   Name=EBF1; Synonyms=FBL6; OrderedLocusNames=At2g25490;
GN   ORFNames=F13B15.15;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY,
RP   SUBCELLULAR LOCATION, INDUCTION, AND INTERACTION WITH CUL1;
RP   SKP1A/ASK1; SKP1B/ASK2; EIN3 AND EIL1.
RX   PubMed=14675533; DOI=10.1016/S0092-8674(03)00968-1;
RA   Potuschak T., Lechner E., Parmentier Y., Yanagisawa S., Grava S.,
RA   Koncz C., Genschik P.;
RT   "EIN3-dependent regulation of plant ethylene hormone signaling by two
RT   Arabidopsis F box proteins: EBF1 and EBF2.";
RL   Cell 115:679-689(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083487; PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L.,
RA   Moffat K.S., Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L.,
RA   Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H.,
RA   Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D.,
RA   Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M.,
RA   Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 245-384.
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   MEDLINE=20530940; PubMed=11077244; DOI=10.1016/S1360-1385(00)01769-6;
RA   Xiao W., Jang J.-C.;
RT   "F-box proteins in Arabidopsis.";
RL   Trends Plant Sci. 5:454-457(2000).
CC   -!- FUNCTION: Component of SCF(EBF1) E3 ubiquitin ligase complexes,
CC       which may mediate the ubiquitination and subsequent proteasomal
CC       degradation of target proteins (probably including EIN3 and EIL1).
CC       Regulator of the ethylene signaling cascade by modulating the
CC       stability of EIN3 and EIL1 proteins. Confers insensitivity to
CC       ethylene.
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBUNIT: Part of a SCF (SKP1-cullin-F-box) protein ligase complex.
CC       Interacts with CUL1, SKP1A/ASK1, SKP1B/ASK2, EIN3, and EIL1.
CC   -!- INTERACTION:
CC       P43291:ASK1; NbExp=1; IntAct=EBI-401198, EBI-401164;
CC       O49484:AT4g34210; NbExp=2; IntAct=EBI-401198, EBI-401185;
CC       Q94AH6:CUL1; NbExp=1; IntAct=EBI-401198, EBI-532411;
CC       O24606:EIN3; NbExp=1; IntAct=EBI-401198, EBI-593576;
CC       Q39255:SKP1A; NbExp=3; IntAct=EBI-401198, EBI-532357;
CC       Q9FHW7:SKP1B; NbExp=3; IntAct=EBI-401198, EBI-604076;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Ubiquitous.
CC   -!- INDUCTION: EIN3-dependent induction by ethylene.
CC   -!- SIMILARITY: Contains 1 F-box domain.
CC   -!- SIMILARITY: Contains 16 LRR (leucine-rich) repeats.
CC   -!- WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database
CC       for the ubiquitin/26S proteasome proteolytic pathway in plants;
CC       URL="http://plantsubq.genomics.purdue.edu/";
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DR   EMBL; AJ609238; CAE75864.1; -; Genomic_RNA.
DR   EMBL; AC006300; AAD20708.1; -; Genomic_DNA.
DR   EMBL; AY072205; AAL60026.1; -; mRNA.
DR   EMBL; AY091333; AAM14272.1; -; mRNA.
DR   EMBL; AK229995; BAF01819.1; -; mRNA.
DR   PIR; A84649; A84649.
DR   RefSeq; NP_565597.1; -.
DR   UniGene; At.24288; -.
DR   IntAct; Q9SKK0; -.
DR   GeneID; 817087; -.
DR   GenomeReviews; CT485783_GR; AT2G25490.
DR   KEGG; ath:AT2G25490; -.
DR   NMPDR; fig|3702.1.peg.9563; -.
DR   TAIR; At2g25490; -.
DR   ArrayExpress; Q9SKK0; -.
DR   GO; GO:0005634; C:nucleus; TAS:TAIR.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0010105; P:negative regulation of ethylene mediated si...; TAS:TAIR.
DR   InterPro; IPR001810; F-box.
DR   InterPro; IPR001611; LRR.
DR   InterPro; IPR006553; LRR_cys_sub-typ.
DR   Pfam; PF00646; F-box; 1.
DR   Pfam; PF00560; LRR_1; 3.
DR   SMART; SM00256; FBOX; 1.
DR   SMART; SM00367; LRR_CC; 1.
DR   PROSITE; PS50181; FBOX; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Complete proteome; Ethylene signaling pathway; Leucine-rich repeat;
KW   Nucleus; Repeat; Ubl conjugation pathway.
FT   CHAIN         1    628       EIN3-binding F-box protein 1.
FT                                /FTId=PRO_0000272245.
FT   DOMAIN       61    109       F-box.
FT   REPEAT       78     95       LRR 1.
FT   REPEAT       96    149       LRR 2.
FT   REPEAT      150    176       LRR 3.
FT   REPEAT      177    203       LRR 4.
FT   REPEAT      204    246       LRR 5.
FT   REPEAT      247    273       LRR 6.
FT   REPEAT      274    297       LRR 7.
FT   REPEAT      298    322       LRR 8.
FT   REPEAT      323    351       LRR 9.
FT   REPEAT      352    377       LRR 10.
FT   REPEAT      378    402       LRR 11.
FT   REPEAT      403    457       LRR 12.
FT   REPEAT      458    484       LRR 13.
FT   REPEAT      485    509       LRR 14.
FT   REPEAT      510    531       LRR 15.
FT   REPEAT      532    555       LRR 16.
SQ   SEQUENCE   628 AA;  66589 MW;  805CD1A3D441F392 CRC64;
     MSQIFSFAGE NDFYRRGAIY PNPKDASLLL SLGSFADVYF PPSKRSRVVA PTIFSAFEKK
     PVSIDVLPDE CLFEIFRRLS GPQERSACAF VSKQWLTLVS SIRQKEIDVP SKITEDGDDC
     EGCLSRSLDG KKATDVRLAA IAVGTAGRGG LGKLSIRGSN SAKVSDLGLR SIGRSCPSLG
     SLSLWNVSTI TDNGLLEIAE GCAQLEKLEL NRCSTITDKG LVAIAKSCPN LTELTLEACS
     RIGDEGLLAI ARSCSKLKSV SIKNCPLVRD QGIASLLSNT TCSLAKLKLQ MLNVTDVSLA
     VVGHYGLSIT DLVLAGLSHV SEKGFWVMGN GVGLQKLNSL TITACQGVTD MGLESVGKGC
     PNMKKAIISK SPLLSDNGLV SFAKASLSLE SLQLEECHRV TQFGFFGSLL NCGEKLKAFS
     LVNCLSIRDL TTGLPASSHC SALRSLSIRN CPGFGDANLA AIGKLCPQLE DIDLCGLKGI
     TESGFLHLIQ SSLVKINFSG CSNLTDRVIS AITARNGWTL EVLNIDGCSN ITDASLVSIA
     ANCQILSDLD ISKCAISDSG IQALASSDKL KLQILSVAGC SMVTDKSLPA IVGLGSTLLG
     LNLQQCRSIS NSTVDFLVER LYKCDILS
//