ID   RCE1_ARATH              Reviewed;         184 AA.
AC   Q9SDY5; O23202; Q0WLB1;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   22-JUL-2008, entry version 49.
DE   RecName: Full=NEDD8-conjugating enzyme Ubc12;
DE            EC=6.3.2.-;
DE   AltName: Full=RUB1-conjugating enzyme 1;
DE   AltName: Full=RUB1-protein ligase 1;
DE   AltName: Full=RUB1 carrier protein 1;
GN   Name=RCE1; OrderedLocusNames=At4g36800; ORFNames=C7A10.560;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND FUNCTION.
RX   MEDLINE=20079655; PubMed=10611386; DOI=10.1073/pnas.96.26.15342;
RA   del Pozo J.C., Estelle M.;
RT   "The Arabidopsis cullin AtCUL1 is modified by the ubiquitin-related
RT   protein RUB1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:15342-15347(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20083488; PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G.,
RA   Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N.,
RA   Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M.,
RA   Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M.,
RA   Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T.,
RA   Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I.,
RA   Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P.,
RA   Langham S.-A., McCullagh B., Bilham L., Robben J.,
RA   van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F.,
RA   Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E.,
RA   Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W.,
RA   Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P.,
RA   Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H.,
RA   De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R.,
RA   van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S.,
RA   Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R.,
RA   Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S.,
RA   Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H.,
RA   Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A.,
RA   Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R.,
RA   Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S.,
RA   Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E.,
RA   Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A.,
RA   Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T.,
RA   Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C.,
RA   Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S.,
RA   Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K.,
RA   Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L.,
RA   Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J.,
RA   Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J.,
RA   Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D.,
RA   Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D.,
RA   Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C.,
RA   Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C.,
RA   Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R.,
RA   Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S.,
RA   Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A.,
RA   Chen E., Marra M.A., Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 402:769-777(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J.,
RA   Hayashizaki Y., Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   TISSUE SPECIFICITY, FUNCTION, AND INTERACTION WITH RBX1.
RX   MEDLINE=22568282; PubMed=12682009; DOI=10.1093/emboj/cdg190;
RA   Dharmasiri S., Dharmasiri N., Hellmann H., Estelle M.;
RT   "The RUB/Nedd8 conjugation pathway is required for early development
RT   in Arabidopsis.";
RL   EMBO J. 22:1762-1770(2003).
CC   -!- FUNCTION: Accepts the ubiquitin-like protein NEDD8/RUB1 from the
CC       ECR1-AXR1 E1 complex and catalyzes its covalent attachment to
CC       other proteins.
CC   -!- CATALYTIC ACTIVITY: ATP + NEDD8 + protein lysine = AMP +
CC       diphosphate + protein N-NEDD8yllysine.
CC   -!- PATHWAY: Protein modification; protein neddylation.
CC   -!- SUBUNIT: Interacts with RBX1.
CC   -!- INTERACTION:
CC       P42744:AXR1; NbExp=1; IntAct=EBI-595116, EBI-595161;
CC       Q94AH6:CUL1; NbExp=1; IntAct=EBI-595116, EBI-532411;
CC       Q940X7:RBX1A; NbExp=2; IntAct=EBI-595116, EBI-532404;
CC       Q570C0:TIR1; NbExp=1; IntAct=EBI-595116, EBI-307183;
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q9SDY5-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q9SDY5-2; Sequence=VSP_034924;
CC         Note=May be due to intron retention. No experimental
CC         confirmation available;
CC   -!- TISSUE SPECIFICITY: Expressed in shoot, root and floral meristems,
CC       and in vascular tissues of leaves.
CC   -!- MISCELLANEOUS: Reduction in RCE1 levels leads to reduced organ
CC       length and defects in gravitropism.
CC   -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family.
CC       UBC12 subfamily.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB16820.1; Type=Erroneous gene model prediction;
CC       Sequence=CAB80346.1; Type=Erroneous gene model prediction;
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DR   EMBL; AF202771; AAF19827.1; -; mRNA.
DR   EMBL; Z99708; CAB16820.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL161590; CAB80346.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AY048210; AAK82473.1; -; mRNA.
DR   EMBL; AY097381; AAM19897.1; -; mRNA.
DR   EMBL; AK230295; BAF02096.1; -; mRNA.
DR   PIR; E85434; E85434.
DR   HSSP; P52490; 1JAT.
DR   IntAct; Q9SDY5; -.
DR   GenomeReviews; CT486007_GR; AT4G36800.
DR   TAIR; At4g36800; -.
DR   GermOnline; AT4G36800; Arabidopsis thaliana.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR015580; Ubc12.
DR   InterPro; IPR016135; UBQ-conjugat/RWD-like.
DR   InterPro; IPR000608; UBQ-conjugat_E2.
DR   Gene3D; G3DSA:3.10.110.10; UBQ-conjugat_E2; 1.
DR   PANTHER; PTHR11621:SF17; Ubc12; 1.
DR   PANTHER; PTHR11621; UBQ-conjugat_E2; 1.
DR   Pfam; PF00179; UQ_con; 1.
DR   ProDom; PD000461; UBQ_conjugat; 1.
DR   SMART; SM00212; UBCc; 1.
DR   PROSITE; PS00183; UBIQUITIN_CONJUGAT_1; 1.
DR   PROSITE; PS50127; UBIQUITIN_CONJUGAT_2; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Complete proteome; Ligase;
KW   Ubl conjugation pathway.
FT   CHAIN         1    184       NEDD8-conjugating enzyme Ubc12.
FT                                /FTId=PRO_0000082494.
FT   ACT_SITE    113    113       Glycyl thioester intermediate (By
FT                                similarity).
FT   VAR_SEQ     102    184       YHPNIDLEGNVCLNILREDWKPVLNINTVIYGLFHLFTEPN
FT                                SEDPLNHDAAAVLRDNPKLFETNVRRAMTGGYVGQTFFPRC
FT                                I -> GLSSFYMPYIVYSFYFKIVCVVETLCWFSCLGLSSQ
FT                                YRFGRKRLPEHL (in isoform 2).
FT                                /FTId=VSP_034924.
SQ   SEQUENCE   184 AA;  20787 MW;  9F541991CED5C1E4 CRC64;
     MIGLFKVKEK QREQAQNATR GGASVKKQSA GELRLHKDIS ELNLPSSCSI SFPNGKDDLM
     NFEVSIKPDD GYYHNGTFVF TFQVSPVYPH EAPKVKCKTK VYHPNIDLEG NVCLNILRED
     WKPVLNINTV IYGLFHLFTE PNSEDPLNHD AAAVLRDNPK LFETNVRRAM TGGYVGQTFF
     PRCI
//