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UniProtKB/Swiss-Prot entry Q9SA14


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU31_ARATH
Primary accession number Q9SA14
Secondary accession number Q1EBW0
Integrated into Swiss-Prot on July 11, 2002
Sequence was last modified on June 12, 2007 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 53)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase 1, chloroplastic [Precursor]
Synonyms 3-IPM-DH 1
IMDH 1
EC 1.1.1.85
Beta-IPM dehydrogenase 1
Gene name
Name: IMDH1
OrderedLocusNames: At1g31180
ORFNames: F28K20.14
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
"Arabidopsis ORF clones.";
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AC004793; AAD21684.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT025974; ABG25063.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H86437; H86437.
RefSeq NP_174403.1; -.
UniGene At.40403
3D structure databases
HSSP Q56268; 1A05. [HSSP ENTRY / PDB]
ModBase Q9SA14.
Organism-specific databases
TAIR At1g31180; -.
Gene expression databases
ArrayExpress Q9SA14; -.
GermOnline AT1G31180; Arabidopsis thaliana.
Family and domain databases
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS Q9SA14.
Proteomic databases
ProMEX Q9SA14; -.
Genome annotation databases
GeneID 840006; -.
GenomeReviews CT485782_GR; AT1G31180.
KEGG ath:AT1G31180; -.
NMPDR fig|3702.1.peg.3461; -.
Other
ProtoNet Q9SA14.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Chloroplast; Complete proteome; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    34  34     Chloroplast (Potential). 
CHAIN   35   404  370     3-isopropylmalate dehydrogenase 1, chloroplastic. PRO_0000014453
NP_BIND   117   130  14     NAD (By similarity). 
NP_BIND   323   335  13     NAD (By similarity). 
METAL   265   265        Magnesium or manganese (By similarity). 
METAL   289   289        Magnesium or manganese (By similarity). 
METAL   293   293        Magnesium or manganese (By similarity). 
BINDING   137   137        Substrate (By similarity). 
BINDING   147   147        Substrate (By similarity). 
BINDING   175   175        Substrate (By similarity). 
BINDING   265   265        Substrate (By similarity). 
SITE   182   182  1     Important for catalysis (By similarity). 
SITE   233   233  1     Important for catalysis (By similarity). 
Sequence information
Length: 404 AA [This is the length of the unprocessed precursor] Molecular weight: 43847 Da [This is the MW of the unprocessed precursor] CRC64: D0A7E06E85AC40D0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAAFLQTNIR LEIIPGRYSS LTDHKFRAPY RIRCAAASPV KKRYNITLLP GDGIGPEVIS 

        70         80         90        100        110        120 
VAKNVLQKAG FLQGLEFDFQ EMPFGGAALD LVGVPLPEET STAAKQSDAI LLGAIGGYKW 

       130        140        150        160        170        180 
DKNEKHLRPE MGLLNIRRDL NVFANLRPAT VLPQLVDAST LKKEVAQGVD MMIVRELTGG 

       190        200        210        220        230        240 
IYFGEPRGIT INENGEEVGF NTEIYAAHEI DRIARVAFET ARKRRGKLCS VDKANVLDAS 

       250        260        270        280        290        300 
ILWRKRVTAL ASEYPDVELS HMYVDNAAMQ LVRDPKQFDT IVTNNIFGDI LSDEASMITG 

       310        320        330        340        350        360 
SIGMLPSASL GESGPGLFEP IHGSAPDIAG QDKANPLATI LSAAMLLKYG LGEEKAAKMI 

       370        380        390        400 
EDAVVDALNK GFRTGDIYSP GNKLVGCKEM GEEVLKSVDS KVPV 

Q9SA14 in FASTA format

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