ID   NSF_RAT                 Reviewed;         744 AA.
AC   Q9QUL6;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   22-JUL-2008, entry version 50.
DE   RecName: Full=Vesicle-fusing ATPase;
DE            EC=3.6.4.6;
DE   AltName: Full=N-ethylmaleimide-sensitive fusion protein;
DE            Short=NEM-sensitive fusion protein;
DE   AltName: Full=Vesicular-fusion protein NSF;
GN   Name=Nsf; Synonyms=Erg1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley; TISSUE=Brain;
RA   Guan Z., Lu L.R., Zheng Z.C., Liu X.Y.;
RL   Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RA   Viswanathan V., Vincent S.R.;
RL   Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 11-27; 69-87; 170-187; 218-232; 255-266; 316-335;
RP   338-357; 404-413; 435-446; 534-549; 556-566; 595-631 AND 708-725, AND
RP   MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Hippocampus, and Spinal cord;
RA   Lubec G., Afjehi-Sadat L., Diao W.;
RL   Submitted (APR-2007) to UniProtKB.
CC   -!- FUNCTION: Required for vesicle-mediated transport. Catalyzes the
CC       fusion of transport vesicles within the Golgi cisternae. Is also
CC       required for transport from the endoplasmic reticulum to the Golgi
CC       stack. Seem to function as a fusion protein required for the
CC       delivery of cargo proteins to all compartments of the Golgi stack
CC       independent of vesicle origin (By similarity).
CC   -!- CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate.
CC   -!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
CC   -!- SUBUNIT: Homohexamer. Interacts with GABARAP and GABARAPL2 (By
CC       similarity).
CC   -!- INTERACTION:
CC       P60517:Gabarap; NbExp=1; IntAct=EBI-925794, EBI-925812;
CC       P19491:Gria2; NbExp=4; IntAct=EBI-925794, EBI-77718;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the AAA ATPase family.
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DR   EMBL; AF142097; AAD39485.1; -; mRNA.
DR   EMBL; AF189019; AAF01051.1; -; mRNA.
DR   RefSeq; NP_068516.1; -.
DR   UniGene; Rn.13345; -.
DR   HSSP; P18708; 1NSF.
DR   SMR; Q9QUL6; 1-201, 489-735.
DR   IntAct; Q9QUL6; -.
DR   PhosphoSite; Q9QUL6; -.
DR   Ensembl; ENSRNOG00000003905; Rattus norvegicus.
DR   GeneID; 60355; -.
DR   KEGG; rno:60355; -.
DR   RGD; 621594; Nsf.
DR   HOVERGEN; Q9QUL6; -.
DR   ArrayExpress; Q9QUL6; -.
DR   GermOnline; ENSRNOG00000003905; Rattus norvegicus.
DR   GO; GO:0043198; C:dendritic shaft; IDA:UniProtKB.
DR   GO; GO:0014069; C:postsynaptic density; TAS:UniProtKB.
DR   GO; GO:0016887; F:ATPase activity; IPI:UniProtKB.
DR   GO; GO:0008022; F:protein C-terminus binding; IPI:UniProtKB.
DR   GO; GO:0007269; P:neurotransmitter secretion; TAS:UniProtKB.
DR   GO; GO:0006906; P:vesicle fusion; TAS:UniProtKB.
DR   InterPro; IPR003593; AAA+_ATPase_core.
DR   InterPro; IPR003959; AAA_ATPase_core.
DR   InterPro; IPR003960; AAA_ATPase_CS.
DR   InterPro; IPR003338; ATPaseVAT_N.
DR   InterPro; IPR004201; Cdc48_2.
DR   InterPro; IPR001984; Peptidase_S16.
DR   Pfam; PF00004; AAA; 2.
DR   Pfam; PF02933; CDC48_2; 1.
DR   Pfam; PF02359; CDC48_N; 1.
DR   PRINTS; PR00830; ENDOLAPTASE.
DR   SMART; SM00382; AAA; 2.
DR   PROSITE; PS00674; AAA; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; Direct protein sequencing; Hydrolase;
KW   Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein;
KW   Protein transport; Repeat; Transport.
FT   CHAIN         1    744       Vesicle-fusing ATPase.
FT                                /FTId=PRO_0000263087.
FT   NP_BIND     260    267       ATP (Potential).
FT   NP_BIND     543    550       ATP (Potential).
FT   METAL       550    550       Magnesium (By similarity).
FT   MOD_RES     259    259       Phosphotyrosine (By similarity).
SQ   SEQUENCE   744 AA;  82652 MW;  82978187FC3E0E03 CRC64;
     MAGRTMQAAR CPTDELSLSN CAVVNEKDYQ SGQHVMVRTS PNHKYIFTLR THPSVVPGCI
     AFSLPQRKWA GLSIGQDIEV ALYSFDKAKQ CIGTMTIEID FLQKKNIDSN PYDTDKMAAE
     FIQQFNHQAF SVGQQLVFSF NDKLFGLLVK DIEAMDPSIL KGEPASGKRQ KIEVGLVVGN
     SQVAFEKAEN SSLNLIGKAK TKENRQSIIN PDWNFEKMGI GGLDKEFSDI FRRAFASRVF
     PPEIVEQMGC KHVKGILLYG PPGCGKTLLA RQIGKMLNAR EPKVVNGPEI LNKYVGESEA
     NIRKLFADAE EEQRRLGANS GLHIIIFDEI DAICKQRGSM AGSTGVHDTV VNQLLSKIDG
     VEQLNNILVI GMTNRPDLID EALLRPGRLE VKMEIGLPDE KGRLQILHIH TARMRGHQLL
     SADVDIKELA VETKNFSGAE LEGLVRAAQS TAMNRHIKAS TKVEVDMEKA ESLQVTRGDF
     LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPLVSVL
     LEGPPHSGKT ALAAKIAEES NFPFIKICSP DKMIGFSETA KCQAMKKIFD DAYKSQLSCV
     VVDDIERLLD YVPIGPRFSN LVLQALLVLL KKAPPQGRKL LIIGTTSRKD VLQEMEMLNA
     FSTTIHVPNI ATGEQLLEAL ELLGNFKDKE RTTIAQQVKG KKVWIGIKKL LMLIEMSLQM
     DPEYRVRKFL ALMREEGASP LDFD
//