RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Snakehead rhabdovirus (SHRV)

[TaxID: 103603]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Novirhabdovirus.

Virus host

Gobiosoma bosc (naked goby) [TaxID: 203314]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1128/JVI.74.5.2343-2350.2000; PubMed=10666265 [NCBI, ExPASy, EBI, Israel, Japan]
Johnson M.C., Simon B.E., Kim C.H., Leong J.A.;
"Production of recombinant snakehead rhabdovirus: the NV protein is not required for viral replication.";
J. Virol. 74:2343-2350(2000).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF147498; AAD56771.1; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_050585.1; -.

3D structure databases

ModBase

Q9QJT4.

Family and domain databases

InterPro

IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9QJT4.

Genome annotation databases

GeneID

1457774; -.

Other

ProtoNet

Q9QJT4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1983`	`1983`	`Large structural protein.`	`PRO_0000297840`
`DOMAIN`	`559 756`	`198`	`RdRp catalytic.`

Sequence information

Length: 1983 AA [This is the length of the unprocessed precursor]

Molecular weight: 225025 Da [This is the MW of the unprocessed precursor]

CRC64: 3158F671B780B50D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEFFDLDLEV SQERLPAECS LNAPLNLSLS LQLFGRIEPK TENIRRQSRR ITKVLRERHN 

        70         80         90        100        110        120 
GYRLQDLIID STRTQANLIP HLVSSASGDL NTPILEHWEM LSKYYQSLGY SLPSLDKFDF 

       130        140        150        160        170        180 
KESAGYWNAA CSFRDMLLKS QKVEKKVNKQ QTYVIYDITF EFVEGVVFIH GGEDGFNDGF 

       190        200        210        220        230        240 
LAGGAIAAMT YTELLALFKI LNQRAQALLM CNISKGLEPD MVPSPSTIHS IYAEADHMLR 

       250        260        270        280        290        300 
MAGQGAIDLL KLWEPLVLTQ LGDVLGDRFG LEDDFKLTIR TEAALLADQL NLKRSFARMT 

       310        320        330        340        350        360 
ELIKSETRKQ PLFQLFGLFK HFAYPRVYSR DTINTILGVS DKPSANDPEE YLHDQCEIRK 

       370        380        390        400        410        420 
EFYTRYVKAY HRAPQLDLGG LSPGSYLRRA LEAGKMPNEK SPLYTNLEWF FVKFKKSIEW 

       430        440        450        460        470        480 
PLSDTLSTFL SDKAITQNRS TWLDNETSSR DNSEKRLLLK FIKENEDSVA RVVAQAKEIY 

       490        500        510        520        530        540 
DNEDDRIIAL KVKEMELKLK GRGFGLMTFK PRLLQVLRES IAKKTSKLFP EITMTASDLD 

       550        560        570        580        590        600 
LKKRKFLVSR KSDDRRGYVH MSKSLDINKF CTSQRQFNSQ AVFQCLDELL GTGALFSRVH 

       610        620        630        640        650        660 
EIFEKTWIVD GSASDPPNLK KFKDRYQKLK DLGIDAPHTW GDGVFSGLMG GIEGLCQYVW 

       670        680        690        700        710        720 
TICLLLRVER VLSKTSLTHF VMAQGDNVII NLIIPIEIER DGSISPSEHR RVKSLSTSID 

       730        740        750        760        770        780 
TQLAMELEKS GLTLKIEETL SSEHISIYGK DLHCPHHLTL SLKKAGSASI ISSEQYQDVP 

       790        800        810        820        830        840 
TFLAGLSTSI ETISECVNDK VSAHLFGVIL AHAGWKSLCV SQTWKGWEYP YQKDETINRV 

       850        860        870        880        890        900 
RSQGIKLTEG EQVTVEKRLE RNPDKRCLEW ILATSFLGSA LGMLPFPTPV DLEKRGVGDY 

       910        920        930        940        950        960 
ITHRLALTKK ALSSRVLPRR IEKLIRSMVN LPHSRETDLA KLFDSPFSLN LATEEDATSV 

       970        980        990       1000       1010       1020 
IKRLARSTLR DLDIKNERLR AHIDIMDRGL QDLDRELGDS ETINPRVAHL IRDITDEKES 

      1030       1040       1050       1060       1070       1080 
EMFVTKFATA RTMRTVALEN PQDVSVVSLL NKKSRAKETY TIWRSKRAPA EDWECSTQRA 

      1090       1100       1110       1120       1130       1140 
KIERDSSWGK NVIGVTSPSP VEAMSYRLVD PSTWEEEKKD QDFTINYYLS KPSLISHQAR 

      1150       1160       1170       1180       1190       1200 
LERGPLVPYY GTQTQPLIAK AYNELKGNPK TNKALMLLSL RETIVKSGSN LDKLIMRLCE 

      1210       1220       1230       1240       1250       1260 
RALDLDLNTL PSLRAQEEAS SGEGIRGGIK ESMSPVGPDN FYTNITHKVF NRRWLSGFHI 

      1270       1280       1290       1300       1310       1320 
NIADFIIWGL TCTRKSITTQ GTLSGNLPIC IPACRGCLRR KEREFLDIDN PPRWECKEGV 

      1330       1340       1350       1360       1370       1380 
KDKAYMYFTT WCDLPRLSTL PSLDPQDAVF MIGRQLACSK GQDSGAATKF YNVSPESLGL 

      1390       1400       1410       1420       1430       1440 
LHPRMLLLGY SEGLIFSYLR SQHIVLGCLY HPTITELLPS LEKYTLETID QHARQLGYLF 

      1450       1460       1470       1480       1490       1500 
QEEETAKELL NAGLCPYTPR AIPLTITELK NAVCITVSRS ISVTLETKKS IHLMPESGIS 

      1510       1520       1530       1540       1550       1560 
EEEVVAGRHA ARTLGGLLNI KVPNLVYIDC DLTKGLLPWE PELPSEVLQS ENFKIDGKRV 

      1570       1580       1590       1600       1610       1620 
TLYAKSQKRD NSIWEERGWT CSNSREILAK GVKTKSLFIH QSVPSHLDID PALIVVIGGG 

      1630       1640       1650       1660       1670       1680 
LGGCVVPYLQ HWRRPPVIFT TLFSERERIS EDGDLIIPPE LLVRGLSGRM VERELLEAEL 

      1690       1700       1710       1720       1730       1740 
CDVTVPGNRK AIVDAVKRRI KPNESVLLID EIENRGDAED VLQQSISTLL QSLEKHCSLT 

      1750       1760       1770       1780       1790       1800 
SVHTIRESNV KHFTQRLNIL RRGRYEANLF WNRYNRRDQY EALIVIPSES RMTECTFSVA 

      1810       1820       1830       1840       1850       1860 
SVQAAFQKID DGIEVEAKLE AHSWGLPELP PREKKILLGY VSSVFLKLGL VVIERHMSST 

      1870       1880       1890       1900       1910       1920 
KLIDLLESAG PQMISWEEKQ THRSWASTDS IKEKGVTQDR IMALLCFAWT LKGLKHGVWD 

      1930       1940       1950       1960       1970       1980 
TNMDAVVEKT VYITHGPRLC ALDEKPRVQY AEFKLQSKKR VEDLKGYLGA LLHLETFFPL 


GDR

Q9QJT4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools