ID   LDHA_DANRE              Reviewed;         333 AA.
AC   Q9PVK5;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-NOV-2008, entry version 59.
DE   RecName: Full=L-lactate dehydrogenase A chain;
DE            Short=LDH-A;
DE            EC=1.1.1.27;
GN   Name=ldha;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Cyprinidae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L.;
RT   "Molecular evolution of vertebrate lactate dehydrogenase isozymes by
RT   gene duplication.";
RL   Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; AF067201; AAF02212.1; -; mRNA.
DR   EMBL; BC067188; AAH67188.1; -; mRNA.
DR   RefSeq; NP_571321.1; -.
DR   UniGene; Dr.4212; -.
DR   HSSP; P00338; 1I10.
DR   SMR; Q9PVK5; 2-333.
DR   Ensembl; ENSDARG00000040856; Danio rerio.
DR   GeneID; 30496; -.
DR   KEGG; dre:30496; -.
DR   ZFIN; ZDB-GENE-991026-5; ldha.
DR   HOGENOM; Q9PVK5; -.
DR   HOVERGEN; Q9PVK5; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:InterPro.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0001666; P:response to hypoxia; IDA:ZFIN.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR011304; L-lactate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    333       L-lactate dehydrogenase A chain.
FT                                /FTId=PRO_0000168429.
FT   NP_BIND      30     58       NAD (By similarity).
FT   ACT_SITE    194    194       Proton acceptor (By similarity).
FT   BINDING     100    100       NAD (By similarity).
FT   BINDING     107    107       Substrate (By similarity).
FT   BINDING     139    139       NAD or substrate (By similarity).
FT   BINDING     170    170       Substrate (By similarity).
FT   BINDING     249    249       Substrate (By similarity).
SQ   SEQUENCE   333 AA;  36246 MW;  B5A040A6DDC014BC CRC64;
     MASTKEKLIA HVSKEQPAGP TNKVTVVGVG MVGMAAAVSI LLKDLTDELA LVDVMEDKLK
     GEAMDLQHGS LFLKTHKIVA DKDYSVTANS KVVVVTAGAR QQEGESRLNL VQRNVNIFKF
     IIPNIIKYSP NCILLVVSNP VDILTYVAWK LSGLPRNRVI GSGTNLDSAR FRYLMGEKLG
     IHPSSCHGWV VGEHGDSSVP VWSGVNVAGV SLQALNPDLG TDKDKEDWKS VHKMVVDSAY
     EVIKLKGYTS WAIGMSVADL CESILKNMHK CHPVSTLVKG MHGVNEEVFL SVPCILGNNG
     LTDVVHMTLK PEEEKQLVKS AETLWGVQKE LTL
//