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UniProtKB/Swiss-Prot entry Q9P804


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TRM1_SCHPO
Primary accession number Q9P804
Secondary accession number O74566
Integrated into Swiss-Prot on January 11, 2001
Sequence was last modified on October 1, 2000 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 50)
Name and origin of the protein
Protein name N(2),N(2)-dimethylguanosine tRNA methyltransferase
Synonyms EC 2.1.1.32
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(m(2,2)G26)dimethyltransferase
Gene name
Name: trm1
ORFNames: SPBC25D12.05
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
DOI=10.1016/S0014-5793(99)01679-8; PubMed=10611485 [NCBI, ExPASy, EBI, Israel, Japan]
Niederberger C., Graub R., Costa A., Desgres J., Schweingruber M.E.;
"The tRNA N2,N2-dimethylguanosine-26 methyltransferase encoded by gene trm1 increases efficiency of suppression of an ochre codon in Schizosaccharomyces pombe.";
FEBS Lett. 464:67-70(1999).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ224000; CAA11801.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU329671; CAA20101.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T39993; T39993.
T46565; T46565.
RefSeq NP_596547.1; -.
3D structure databases
ModBase Q9P804.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-004543-MON; -.
Organism-specific databases
GeneDB_Spombe SPBC25D12.05; -.
Gene expression databases
ArrayExpress Q9P804; -.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from GeneDB_SPombe).
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0004809; Molecular function: tRNA (guanine-N2-)-methyltransferase activity (inferred from electronic annotation from InterPro).
GO:0008033; Biological process: tRNA processing (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR002905; TRM_MeTrfase.
Graphical view of domain structure.
PANTHER PTHR10631; TRM_mtfrase; 1.
Pfam PF02005; TRM; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00308; TRM1; 1.
ProtoNet Q9P804.
Genome annotation databases
GeneID 2540604; -.
KEGG spo:SPBC25D12.05; -.
NMPDR fig|4896.1.peg.2413; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Methyltransferase; Nucleus; S-adenosyl-L-methionine; Transferase; tRNA processing.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   548  548     N(2),N(2)-dimethylguanosine tRNA methyltransferase. PRO_0000147677
CONFLICT   322   322        S -> T (in Ref. 1; CAA11801). 
Sequence information
Length: 548 AA [This is the length of the unprocessed precursor] Molecular weight: 60297 Da [This is the MW of the unprocessed precursor] CRC64: E0DD19178A6B4959 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MYEANMLRLS QRISNAVNHF STAMMSGASA SLTEGSAIIP FSNPNEVFYN PVQQFNRDLS 

        70         80         90        100        110        120 
VTAIRAWSET RSKKIVAKSH HRHQLLDQNS ELSQENLTKC DTTHDFEKNC SEKTDSTSAD 

       130        140        150        160        170        180 
NIAGFTILEA LSATGLRSIR YAKELPNVKR ILANDLLENA VKTIEKNVNY NNVSDIVIPN 

       190        200        210        220        230        240 
KGDANAVMHM NKFHYDVIDL DPYGSAAPFL DAAVQSVSKD GLLCITCTDS AVLAGNAYPE 

       250        260        270        280        290        300 
KCFSNYGGSS LRSNFCHEQA VRHLLYAIAA SAAKYGRAIK PLLSLSIDFY FRVFVQIKAK 

       310        320        330        340        350        360 
PVLVKNLQSQ SLLIYHCSGC GSFAEQPLGK TSPGRLPGTT KFSNASGPPV SANCEHCGYV 

       370        380        390        400        410        420 
HHVGGPLWGG PLHDAEFLKK MRAIAEDLDP EVYGTKRRIL GMLALADEEL PDVPFYFVLS 

       430        440        450        460        470        480 
QICSVLRSQS PPQNIFVSAL LNAGYRVSGS HAKSNAIKTN APWSFVWDVL RSWIKDHPVK 

       490        500        510        520        530        540 
LENISKTSAG AAILEKTPTA EVDFTFRPDS EFASKKEGYT RYQMNPTENW GPKSKPGKRT 


IAEVDSKS 

Q9P804 in FASTA format

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