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UniProtKB/Swiss-Prot entry Q9P2I0


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CPSF2_HUMAN
Primary accession number Q9P2I0
Secondary accession numbers Q6NSJ1 Q9H3W7
Integrated into Swiss-Prot on October 18, 2001
Sequence was last modified on August 16, 2004 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 53)
Name and origin of the protein
Protein name Cleavage and polyadenylation specificity factor subunit 2
Synonyms Cleavage and polyadenylation specificity factor 100 kDa subunit
CPSF 100 kDa subunit
Gene name
Name: CPSF2
Synonyms: CPSF100, KIAA1367
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Testis;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 204-782.
TISSUE=Brain;
DOI=10.1093/dnares/7.1.65; PubMed=10718198 [NCBI, ExPASy, EBI, Israel, Japan]
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.";
DNA Res. 7:65-73(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 279-782.
TISSUE=Lymph node;
The German cDNA consortium;
Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
[4]
FUNCTION IN PRE-MRNA 3'-END PROCESSING, AND IDENTIFICATION IN THE CPSF COMPLEX.
DOI=10.1038/sj.emboj.7600070; PubMed=14749727 [NCBI, ExPASy, EBI, Israel, Japan]
Kaufmann I., Martin G., Friedlein A., Langen H., Keller W.;
"Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase.";
EMBO J. 23:616-626(2004).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-660, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0404720101; PubMed=15302935 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-395; SER-419; SER-420 AND SER-423, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1016/j.cell.2006.09.026; PubMed=17081983 [NCBI, ExPASy, EBI, Israel, Japan]
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.;
"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.";
Cell 127:635-648(2006).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419; SER-420; SER-423 AND SER-660, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0805139105; PubMed=18669648 [NCBI, ExPASy, EBI, Israel, Japan]
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.;
"A quantitative atlas of mitotic phosphorylation.";
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
BC070095; AAH70095.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB037788; BAA92605.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL442079; CAC09445.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_059133.1; -.
UniGene Hs.657632
3D structure databases
ModBase Q9P2I0.
Protein-protein interaction databases
IntAct Q9P2I0; -.
PTM databases
PhosphoSite Q9P2I0; -.
Enzyme and pathway databases
Reactome REACT_1675; mRNA Processing.
REACT_1788; Transcription.
REACT_71; Gene Expression.
Organism-specific databases
H-InvDB HIX0020194; -.
HGNC HGNC:2325; CPSF2.
GenAtlas CPSF2.
HPA HPA000870; -.
MIM 606028; gene. [NCBI / EBI]
PharmGKB PA26842; -.
GeneCards Q9P2I0.
HUGE KIAA1367.
Gene expression databases
ArrayExpress Q9P2I0; -.
CleanEx HS_CPSF2; -.
GermOnline ENSG00000165934; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from HPA).
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0016787; Molecular function: hydrolase activity (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from UniProtKB-KW).
GO:0000398; Biological process: nuclear mRNA splicing, via spliceosome (inferred from experiment from Reactome).
QuickGo view.
Family and domain databases
InterPro IPR001279; Blactmase-like.
IPR011108; RMMBL.
Graphical view of domain structure.
Pfam PF00753; Lactamase_B; 1.
PF07521; RMMBL; 1.
Pfam graphical view of domain structure.
ProtoNet Q9P2I0.
Proteomic databases
PeptideAtlas Q9P2I0; -.
Genome annotation databases
Ensembl ENSG00000165934; Homo sapiens. [Contig view]
GeneID 53981; -.
KEGG hsa:53981; -.
Phylogenomic databases
HOGENOM Q9P2I0; -.
HOVERGEN Q9P2I0; -.
Other
NextBio 56268; -.
SOURCE CPSF2; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
mRNA processing; Nucleus; Phosphoprotein; RNA-binding.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   782  782     Cleavage and polyadenylation specificity factor subunit 2. PRO_0000074393
MOD_RES   395   395        Phosphotyrosine. 
MOD_RES   412   412        Phosphoserine (By similarity). 
MOD_RES   419   419        Phosphoserine. 
MOD_RES   420   420        Phosphoserine. 
MOD_RES   423   423        Phosphoserine. 
MOD_RES   660   660        Phosphoserine. 
CONFLICT   289   289        D -> G (in Ref. 1; AAH70095). 
CONFLICT   654   654        K -> R (in Ref. 1; AAH70095). 
Sequence information
Length: 782 AA [This is the length of the unprocessed precursor] Molecular weight: 88487 Da [This is the MW of the unprocessed precursor] CRC64: F67B4813B9883CE8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTSIIKLTTL SGVQEESALC YLLQVDEFRF LLDCGWDEHF SMDIIDSLRK HVHQIDAVLL 

        70         80         90        100        110        120 
SHPDPLHLGA LPYAVGKLGL NCAIYATIPV YKMGQMFMYD LYQSRHNTED FTLFTLDDVD 

       130        140        150        160        170        180 
AAFDKIQQLK FSQIVNLKGK GHGLSITPLP AGHMIGGTIW KIVKDGEEEI VYAVDFNHKR 

       190        200        210        220        230        240 
EIHLNGCSLE MLSRPSLLIT DSFNATYVQP RRKQRDEQLL TNVLETLRGD GNVLIAVDTA 

       250        260        270        280        290        300 
GRVLELAQLL DQIWRTKDAG LGVYSLALLN NVSYNVVEFS KSQVEWMSDK LMRCFEDKRN 

       310        320        330        340        350        360 
NPFQFRHLSL CHGLSDLARV PSPKVVLASQ PDLECGFSRD LFIQWCQDPK NSIILTYRTT 

       370        380        390        400        410        420 
PGTLARFLID NPSEKITEIE LRKRVKLEGK ELEEYLEKEK LKKEAAKKLE QSKEADIDSS 

       430        440        450        460        470        480 
DESDIEEDID QPSAHKTKHD LMMKGEGSRK GSFFKQAKKS YPMFPAPEER IKWDEYGEII 

       490        500        510        520        530        540 
KPEDFLVPEL QATEEEKSKL ESGLTNGDEP MDQDLSDVPT KCISTTESIE IKARVTYIDY 

       550        560        570        580        590        600 
EGRSDGDSIK KIINQMKPRQ LIIVHGPPEA SQDLAECCRA FGGKDIKVYM PKLHETVDAT 

       610        620        630        640        650        660 
SETHIYQVRL KDSLVSSLQF CKAKDAELAW IDGVLDMRVS KVDTGVILEE GELKDDGEDS 

       670        680        690        700        710        720 
EMQVEAPSDS SVIAQQKAMK SLFGDDEKET GEESEIIPTL EPLPPHEVPG HQSVFMNEPR 

       730        740        750        760        770        780 
LSDFKQVLLR EGIQAEFVGG VLVCNNQVAV RRTETGRIGL EGCLCQDFYR IRDLLYEQYA 


IV 

Q9P2I0 in FASTA format

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