[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CHARACTERIZATION, AND INTERACTION WITH PARD3; CDC42 AND RAC1. TISSUE=B-cell; PubMed=10954424 [NCBI, ExPASy, EBI, Israel, Japan] Johansson A.-S., Driessens M., Aspenstroem P.; "The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1."; J. Cell Sci. 113:3267-3275(2000).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, AND INTERACTION WITH CDC42; RAC1 AND PRKCZ. TISSUE=Cervix carcinoma; DOI=10.1016/S0960-9822(00)00535-2; PubMed=10873802 [NCBI, ExPASy, EBI, Israel, Japan] Qiu R.-G., Abo A., Martin G.S.; "A human homolog of the Caenorhabditis elegans polarity determinant Par-6 links Rac and Cdc42 to PKC-zeta signaling and cell transformation."; Curr. Biol. 10:697-707(2000).
[3]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND INTERACTION WITH ARHQ. TISSUE=Brain; DOI=10.1038/35019573; PubMed=10934474 [NCBI, ExPASy, EBI, Israel, Japan] Joberty G., Petersen C., Gao L., Macara I.G.; "The cell-polarity protein Par6 links Par3 and atypical protein kinase C to Cdc42."; Nat. Cell Biol. 2:531-539(2000).
[4]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND SUBUNIT OF A COMPLEX CONTAINING PARD3 AND PRKCI. TISSUE=Kidney; DOI=10.1083/jcb.152.6.1183; PubMed=11257119 [NCBI, ExPASy, EBI, Israel, Japan] Suzuki A., Yamanaka T., Hirose T., Manabe N., Mizuno K., Shimizu M., Akimoto K., Izumi Y., Ohnishi T., Ohno S.; "Atypical protein kinase C is involved in the evolutionarily conserved par protein complex and plays a critical role in establishing epithelia-specific junctional structures."; J. Cell Biol. 152:1183-1196(2001).
[5]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND INTERACTION WITH RAC1; CDC42; PRKCI AND PRKCZ. TISSUE=Neuroblastoma; PubMed=11260256 [NCBI, ExPASy, EBI, Israel, Japan] Noda Y., Takeya R., Ohno S., Naito S., Ito T., Sumimoto H.; "Human homologues of the Caenorhabditis elegans cell polarity protein PAR6 as an adaptor that links the small GTPases Rac and Cdc42 to atypical protein kinase C."; Genes Cells 6:107-119(2001).
[6]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). TISSUE=Eye; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[7]	NUCLEOTIDE SEQUENCE [MRNA] OF 115-345, AND INTERACTION WITH HTLV-1 TAX. TISSUE=Lymphocyte; DOI=10.1038/sj.onc.1201567; PubMed=9482110 [NCBI, ExPASy, EBI, Israel, Japan] Rousset R., Fabre S., Desbois C., Bantignies F., Jalinot P.; "The C-terminus of the HTLV-1 Tax oncoprotein mediates interaction with the PDZ domain of cellular proteins."; Oncogene 16:643-654(1998).
[8]	INTERACTION WITH MPP5. DOI=10.1038/ncb923; PubMed=12545177 [NCBI, ExPASy, EBI, Israel, Japan] Hurd T.W., Gao L., Roh M.H., Macara I.G., Margolis B.; "Direct interaction of two polarity complexes implicated in epithelial tight junction assembly."; Nat. Cell Biol. 5:137-142(2003).
[9]	INTERACTION WITH CRB3, AND DOMAIN. DOI=10.1091/mbc.E03-04-0235; PubMed=14718572 [NCBI, ExPASy, EBI, Israel, Japan] Lemmers C., Michel D., Lane-Guermonprez L., Delgrossi M.-H., Medina E., Arsanto J.-P., Le Bivic A.; "CRB3 binds directly to Par6 and regulates the morphogenesis of the tight junctions in mammalian epithelial cells."; Mol. Biol. Cell 15:1324-1333(2004).
[10]	X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 14-95 IN COMPLEX WITH PRKCI, AND MUTAGENESIS OF LYS-19; ARG-28 AND ARG-89. DOI=10.1074/jbc.M409823200; PubMed=15590654 [NCBI, ExPASy, EBI, Israel, Japan] Hirano Y., Yoshinaga S., Takeya R., Suzuki N.N., Horiuchi M., Kohjima M., Sumimoto H., Inagaki F.; "Structure of a cell polarity regulator, a complex between atypical PKC and Par6 PB1 domains."; J. Biol. Chem. 280:9653-9661(2005).

Comments

FUNCTION: Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
SUBUNIT: Interacts with MAP2K5 (By similarity). Interacts with PARD3. Interacts with GTP-bound forms of CDC42, ARHQ/TC10 and RAC1. Interacts with the N-terminal part of PRKCI and PRKCZ. Part of a complex with PARD3, CDC42 or RAC1 and PRKCI or PRKCZ. Part of a complex with LLGL1 and PRKCI (By similarity). Interacts with human T-cell leukemia virus type I TAX protein. Interacts with MPP5 and CRB3.
INTERACTION:
P60953:CDC42; NbExp=4; IntAct=EBI-81876, EBI-81752;
Q7KZI7:MARK2; NbExp=1; IntAct=EBI-81876, EBI-516560;
Q96L34:MARK4; NbExp=1; IntAct=EBI-81876, EBI-302319;
Q8TEW0:PARD3; NbExp=2; IntAct=EBI-81876, EBI-81968;
Q9BYG5:PARD6B; NbExp=1; IntAct=EBI-81876, EBI-295391;
P41743:PRKCI; NbExp=4; IntAct=EBI-81876, EBI-286199;
P63000:RAC1; NbExp=1; IntAct=EBI-81876, EBI-413628;
Q04917:YWHAH; NbExp=1; IntAct=EBI-81876, EBI-306940;
P63104:YWHAZ; NbExp=1; IntAct=EBI-81876, EBI-347088;
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane. Cell projection, ruffle. Cell junction, tight junction. Note=Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	Q9NPB6-1
Note: No experimental confirmation available.
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	Q9NPB6-2
Features which should be applied to build the isoform sequence: VSP_007459.

TISSUE SPECIFICITY: Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
DOMAIN: The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases (By similarity).
DOMAIN: The OPR domain mediates interactions with MAP2K5 (By similarity).
DOMAIN: The PDZ domain mediates the interaction with CRB3.
SIMILARITY: Belongs to the PAR6 family.
SIMILARITY: Contains 1 OPR domain.
SIMILARITY: Contains 1 PDZ (DHR) domain.
SIMILARITY: Contains 1 pseudo-CRIB domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ277095; CAB85490.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF265565; AAF75548.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF252292; AAF71529.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB043634; BAA96235.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB041642; BAB16105.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC015626; AAH15626.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF028827; AAB84252.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001032358.1; -.
NP_058644.1; -.

UniGene

Hs.112933

3D structure databases

PDB

1WMH; X-ray; 1.50 A; B=14-95.

[ExPASy / RCSB / EBI]

1WMH; -.

Q9NPB6; 131-253.

Protein-protein interaction databases

IntAct

Q9NPB6; -.

PTM databases

PhosphoSite

Q9NPB6; -.

Organism-specific databases

HIX0013153; -.

HGNC:15943; PARD6A.

CAB009733; -.

607484; gene. [NCBI / EBI]

PharmGKB

PA32937; -.

GeneCards

Q9NPB6.

Gene expression databases

CleanEx

HS_PARD6A; -.

GermOnline

ENSG00000102981; Homo sapiens.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from sequence or structural similarity from UniProtKB).
GO:0005923;	Cellular component: tight junction (inferred from sequence or structural similarity from UniProtKB).
GO:0030742;	Molecular function: GTP-dependent protein binding (inferred from sequence or structural similarity from UniProtKB).
GO:0017048;	Molecular function: Rho GTPase binding (inferred from sequence or structural similarity from UniProtKB).
GO:0008134;	Molecular function: transcription factor binding (traceable author statement from ProtInc).
GO:0045217;	Biological process: cell-cell junction maintenance (inferred from sequence or structural similarity from UniProtKB).
GO:0016032;	Biological process: viral reproduction (traceable author statement from ProtInc).
QuickGo view.

Family and domain databases

InterPro

IPR000270; OPR_PB1.
IPR001478; PDZ.
Graphical view of domain structure.

Pfam

PF00564; PB1; 1.
PF00595; PDZ; 1.
Pfam graphical view of domain structure.

SMART

SM00666; PB1; 1.
SM00228; PDZ; 1.
SMART graphical view of domain structure.

PROSITE

PS50106; PDZ; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9NPB6.

Genome annotation databases

Ensembl

ENSG00000102981; Homo sapiens. [Contig view]

GeneID

50855; -.

KEGG

hsa:50855; -.

Phylogenomic databases

HOGENOM

Q9NPB6; -.

HOVERGEN

Q9NPB6; -.

Other

SOURCE

PARD6A; Homo sapiens.

ProtoNet

Q9NPB6.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Alternative splicing; Cell cycle; Cell division; Cell junction; Cell membrane; Cell projection; Cytoplasm; Membrane; Tight junction.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 346`	`346`	`Partitioning defective 6 homolog alpha.`	`PRO_0000112513`
`DOMAIN`	`15 95`	`81`	`OPR.`
`DOMAIN`	`133 150`	`18`	`Pseudo-CRIB.`
`DOMAIN`	`157 250`	`94`	`PDZ.`
`REGION`	`1 116`	`116`	`Interaction with PRKCI and PRKCZ.`
`REGION`	`126 253`	`128`	`Interaction with PARD3 and CDC42 (By similarity).`
`VAR_SEQ`	`96 96`		`Missing (in isoform 2).`	`VSP_007459`
`MUTAGEN`	`19 19`		`K->A: Loss of interaction with PRKCI.`
`MUTAGEN`	`28 28`		`R->A: Slight decrease of interaction with PRKCI. Loss of interaction with PRKCI; when associated with A-89.`
`MUTAGEN`	`89 89`		`R->A: Slight decrease of interaction with PRKCI. Loss of interaction with PRKCI; when associated with A-28.`
`STRAND`	`15 22`	`8`
`STRAND`	`25 32`	`8`
`HELIX`	`33 35`	`3`
`HELIX`	`39 49`	`11`
`STRAND`	`58 62`	`5`
`STRAND`	`68 70`	`3`
`HELIX`	`74 80`	`7`
`STRAND`	`83 86`	`4`
`STRAND`	`88 93`	`6`

Sequence information

Length: 346 AA [This is the length of the unprocessed precursor]

Molecular weight: 37388 Da [This is the MW of the unprocessed precursor]

CRC64: 9241E0EDC3694AD4 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MARPQRTPAR SPDSIVEVKS KFDAEFRRFA LPRASVSGFQ EFSRLLRAVH QIPGLDVLLG 

        70         80         90        100        110        120 
YTDAHGDLLP LTNDDSLHRA LASGPPPLRL LVQKRAEADS SGLAFASNSL QRRKKGLLLR 

       130        140        150        160        170        180 
PVAPLRTRPP LLISLPQDFR QVSSVIDVDL LPETHRRVRL HKHGSDRPLG FYIRDGMSVR 

       190        200        210        220        230        240 
VAPQGLERVP GIFISRLVRG GLAESTGLLA VSDEILEVNG IEVAGKTLDQ VTDMMVANSH 

       250        260        270        280        290        300 
NLIVTVKPAN QRNNVVRGAS GRLTGPPSAG PGPAEPDSDD DSSDLVIENR QPPSSNGLSQ 

       310        320        330        340 
GPPCWDLHPG CRHPGTRSSL PSLDDQEQAS SGWGSRIRGD GSGFSL

Q9NPB6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools