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UniProtKB/Swiss-Prot entry Q9M9F5


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NCED9_ARATH
Primary accession number Q9M9F5
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 2007
Sequence was last modified on October 1, 2000 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 32)
Name and origin of the protein
Protein name 9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic [Precursor]
Synonyms AtNCED9
EC 1.13.11.51
Gene name
Name: NCED9
OrderedLocusNames: At1g78390
ORFNames: F3F9.10
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[2]
FUNCTION, AND INDUCTION BY DROUGHT STRESS.
PubMed=11532178 [NCBI, ExPASy, EBI, Israel, Japan]
Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.;
"Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.";
Plant J. 27:325-333(2001).
[3]
SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY DROUGHT STRESS.
DOI=10.1046/j.1365-313X.2003.01786.x; PubMed=12834401 [NCBI, ExPASy, EBI, Israel, Japan]
Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K., McCarty D.R.;
"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family.";
Plant J. 35:44-56(2003).
[4]
FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
DOI=10.1111/j.1365-313X.2005.02622.x; PubMed=16412079 [NCBI, ExPASy, EBI, Israel, Japan]
Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E., Marion-Poll A.;
"Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy.";
Plant J. 45:309-319(2006).
Comments
  • FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Contributes probably to abscisic acid synthesis for the induction of seed dormancy.
  • CATALYTIC ACTIVITY: A 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal.
  • CATALYTIC ACTIVITY: 9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al.
  • CATALYTIC ACTIVITY: 9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al.
  • SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
  • TISSUE SPECIFICITY: Expressed in developing siliques, embryo and endosperm.
  • DEVELOPMENTAL STAGE: Expressed in seeds at early and mid-maturation stages.
  • INDUCTION: Low induction by drought stress.
  • MISCELLANEOUS: Loss-of-function mutants (T-DNA insertion) exhibit abscisic-acid-deficient phenotypes in seeds, but not in vegetative tissues.
  • SIMILARITY: Belongs to the carotenoid oxygenase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AC013430; AAF71797.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR E96812; E96812.
RefSeq NP_177960.1; -.
UniGene At.34302
3D structure databases
ModBase Q9M9F5.
Organism-specific databases
TAIR At1g78390; -.
Ontologies
GO
GO:0009507; Cellular component: chloroplast (inferred from electronic annotation from UniProtKB-KW).
GO:0045549; Molecular function: 9-cis-epoxycarotenoid dioxygenase activity (inferred from electronic annotation from EC).
GO:0009688; Biological process: abscisic acid biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR002110; ANK.
IPR004294; Carotenoid_Oase.
Graphical view of domain structure.
PANTHER PTHR10543; Carotenoid_Oase; 1.
Pfam PF03055; RPE65; 1.
Pfam graphical view of domain structure.
PRINTS PR01415; ANKYRIN.
ProtoNet Q9M9F5.
Genome annotation databases
GeneID 844175; -.
GenomeReviews CT485782_GR; AT1G78390.
KEGG ath:AT1G78390; -.
NMPDR fig|3702.1.peg.7389; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Abscisic acid biosynthesis; Chloroplast; Complete proteome; Dioxygenase; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom  To Length Description FTId
TRANSIT   1     ?        Chloroplast (Potential). 
CHAIN   ?   657        9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic. PRO_0000285998
COMPBIAS   70   116  47     Ser-rich. 
Sequence information
Length: 657 AA [This is the length of the unprocessed precursor] Molecular weight: 73015 Da [This is the MW of the unprocessed precursor] CRC64: F41DECBE94806318 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTIITIISGM YIYSLLSQDA HHSQYGQNTN LVLKKPIPKP QTAAFNQEST MASTTLLPST 

        70         80         90        100        110        120 
STQFLDRTFS TSSSSSRPKL QSLSFSSTLR NKKLVVPCYV SSSVNKKSSV SSSLQSPTFK 

       130        140        150        160        170        180 
PPSWKKLCND VTNLIPKTTN QNPKLNPVQR TAAMVLDAVE NAMISHERRR HPHPKTADPA 

       190        200        210        220        230        240 
VQIAGNFFPV PEKPVVHNLP VTGTVPECIQ GVYVRNGANP LHKPVSGHHL FDGDGMVHAV 

       250        260        270        280        290        300 
RFDNGSVSYA CRFTETNRLV QERECGRPVF PKAIGELHGH LGIAKLMLFN TRGLFGLVDP 

       310        320        330        340        350        360 
TGGLGVANAG LVYFNGHLLA MSEDDLPYHV KVTQTGDLET SGRYDFDGQL KSTMIAHPKI 

       370        380        390        400        410        420 
DPETRELFAL SYDVVSKPYL KYFRFTSDGE KSPDVEIPLD QPTMIHDFAI TENFVVIPDQ 

       430        440        450        460        470        480 
QVVFRLPEMI RGGSPVVYDE KKKSRFGILN KNAKDASSIQ WIEVPDCFCF HLWNSWEEPE 

       490        500        510        520        530        540 
TDEVVVIGSC MTPPDSIFNE HDETLQSVLS EIRLNLKTGE STRRPVISEQ VNLEAGMVNR 

       550        560        570        580        590        600 
NLLGRKTRYA YLALTEPWPK VSGFAKVDLS TGEIRKYIYG EGKYGGEPLF LPSGDGEEDG 

       610        620        630        640        650 
GYIMVFVHDE EKVKSELQLI NAVNMKLEAT VTLPSRVPYG FHGTFISKED LSKQALC 

Q9M9F5 in FASTA format

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