[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY. STRAIN=cv. Columbia; DOI=10.1093/nar/28.8.1692; PubMed=10734187 [NCBI, ExPASy, EBI, Israel, Japan] Garcia V., Salanoubat M., Choisne N., Tissier A.; "An ATM homologue from Arabidopsis thaliana: complete genomic organisation and expression analysis."; Nucleic Acids Res. 28:1692-1699(2000).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=cv. Columbia; DOI=10.1038/35048706; PubMed=11130713 [NCBI, ExPASy, EBI, Israel, Japan] Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.; "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."; Nature 408:820-822(2000).
[3]	FUNCTION. DOI=10.1105/tpc.006577; PubMed=12509526 [NCBI, ExPASy, EBI, Israel, Japan] Garcia V., Bruchet H., Camescasse D., Granier F., Bouchez D., Tissier A.; "AtATM is essential for meiosis and the somatic response to DNA damage in plants."; Plant Cell 15:119-132(2003).
[4]	FUNCTION. DOI=10.1101/gad.1333805; PubMed=16166376 [NCBI, ExPASy, EBI, Israel, Japan] Vespa L., Couvillion M., Spangler E., Shippen D.E.; "ATM and ATR make distinct contributions to chromosome end protection and the maintenance of telomeric DNA in Arabidopsis."; Genes Dev. 19:2111-2115(2005).
[5]	FUNCTION. DOI=10.1091/mbc.E04-10-0890; PubMed=15772150 [NCBI, ExPASy, EBI, Israel, Japan] Friesner J.D., Liu B., Culligan K., Britt A.B.; "Ionizing radiation-dependent gamma-H2AX focus formation requires ataxia telangiectasia mutated and ataxia telangiectasia mutated and Rad3-related."; Mol. Biol. Cell 16:2566-2576(2005).

Comments

FUNCTION: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR) or DNA replication stalling. Plays a central role in the perception and response to both stress-induced damage in somatic cells and developmentally programmed DNA damage during meiosis. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates histone variant H2AX to form H2AXS139ph at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Involved in transcriptional regulation of RAD51, PARP1, GR1, and LIG4 in response to DNA double strand breaks. Plays a dual role by activating the DNA damage response at dysfunctional telomeres and yet preventing this activation at functional telomeres. Not required for telomere length homeostasis.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SUBCELLULAR LOCATION: Nucleus (Probable).
TISSUE SPECIFICITY: Ubiquitously expressed at low levels with slightly higher levels in flower buds.
MISCELLANEOUS: Loss-of-function mutation (T-DNA insertion) causes meiotic defects, hypersensitivity to double strand breaks-inducing agents, and an inability to properly induce IR-mediated transcription of several DNA repair genes.
SIMILARITY: Belongs to the PI3/PI4-kinase family.
SIMILARITY: Contains 1 FAT domain.
SIMILARITY: Contains 1 FATC domain.
SIMILARITY: Contains 1 PI3K/PI4K domain.
SIMILARITY: Contains 1 PWWP domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ250248; CAB86487.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL096856; CAB92122.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T51174; T51174.

3D structure databases

ModBase

Q9M3G7.

Organism-specific databases

GeneFarm

4562; -.

TAIR

At3g48190; -.

Gene expression databases

ArrayExpress

Q9M3G7; -.

GermOnline

AT3G48190; Arabidopsis thaliana.

Family and domain databases

InterPro

IPR015519; Ataxia_Telang_Mut.
IPR003152; FATC.
IPR000403; PI3/4_kinase_cat.
IPR014009; PIK_FAT.
IPR000313; PWWP.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.1070.11; PI3/4_kinase_cat; 1.

PANTHER

PTHR11139:SF3; Ataxia_Telang_Mut; 1.

Pfam

PF02260; FATC; 1.
PF00454; PI3_PI4_kinase; 1.
PF00855; PWWP; 1.
Pfam graphical view of domain structure.

SMART

SM00146; PI3Kc; 1.
SMART graphical view of domain structure.

PROSITE

PS51189; FAT; 1.
PS51190; FATC; 1.
PS00915; PI3_4_KINASE_1; 1.
PS00916; PI3_4_KINASE_2; 1.
PS50290; PI3_4_KINASE_3; 1.
PS50812; PWWP; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9M3G7.

Genome annotation databases

GenomeReviews

BA000014_GR; AT3G48190.

Other

ProtoNet

Q9M3G7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell cycle; Chromosomal protein; Complete proteome; DNA damage; DNA repair; DNA-binding; Kinase; Meiosis; Nucleus; Serine/threonine-protein kinase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 3856`	`3856`	`Serine/threonine-protein kinase ATM.`	`PRO_0000088843`
`DOMAIN`	`108 162`	`55`	`PWWP.`
`DOMAIN`	`2727 3393`	`667`	`FAT.`
`DOMAIN`	`3525 3773`	`249`	`PI3K/PI4K.`
`DOMAIN`	`3824 3856`	`33`	`FATC.`
`MOTIF`	`3233 3249`	`17`	`Bipartite nuclear localization signal (Potential).`
`CONFLICT`	`3640 3640`		`P -> S (in Ref. 1; CAB86487).`

Sequence information

Length: 3856 AA [This is the length of the unprocessed precursor]

Molecular weight: 435114 Da [This is the MW of the unprocessed precursor]

CRC64: 934D08DC3C67A180 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKLQNPDKKT LREGFSQESS VVALDSGVLA MSGLKCDGKF PVKDVLMEEG GDKVRKIQVS 

        70         80         90        100        110        120 
GGNISLVVDF SGARTSSNNF FESNASCVNE NLVKGNGYRE DETQEFLVGN LVWVMTKYKK 

       130        140        150        160        170        180 
WWPGEVVDFK ADAKESFMVR SIGQSHLVSW FASSKLKPFK ESFEQVLNQR NDNGFFDALQ 

       190        200        210        220        230        240 
KAMSLLSNSL KLDMTCSCIA DGNGIVSAQN ITTRKNKPLI LREFSVDRLE PKEFVTQLKN 

       250        260        270        280        290        300 
IAKCVLNAGV LESTVMQSQL SAFYTLFGHK QIPMAQLHEN EGRKSFTAKM SDSKFIGSPS 

       310        320        330        340        350        360 
ICAGNSRKRF RKEWFRKFVS EVDNVSARDD LVNVPPSDLI SKLKLLAVGY NCSEETENIG 

       370        380        390        400        410        420 
LFEWFFSKFR ISVYHDENAY KMQLANMAGF KDLMLATNAN RGTVQKTLKS KKIGKSKMEP 

       430        440        450        460        470        480 
LNGVSVADTE QKTFELQISK KSNIESLNGV SVADTEQKTF ELQILEKSNI ESLNGVSTPN 

       490        500        510        520        530        540 
IDHEASKSNN SGKTKINHII GHSNFPSSVA KVQLAKDFQD KLLVQAPDRK AMTADTLSRP 

       550        560        570        580        590        600 
AAILVPDLNS GGNALGTAEF DHMQRPETLI QHNVCPQEEK TPRSTILNFQ VTAHQGVSGT 

       610        620        630        640        650        660 
QFVSSQPTSY KHFTSADLFT YSGKKKRGRK RKNAEELPIV AHASATTGIP DLNGTNTEPT 

       670        680        690        700        710        720 
LVLPQVEPTQ RRRRRKKEES PNGLTRGITI LFLKFSSQVS MPSRDDLTST FSAFGPLDSS 

       730        740        750        760        770        780 
ETHVSEEFSG AQVAFVSSAD AIEAVKSLEK ANPFGETLVN FRLQQKLITV QRNIAPRMPV 

       790        800        810        820        830        840 
ISHVSPVPKP NNIPTSMDAM RQNLLMMTAM LEKSGDSLSR ETKAKLKSEI TGLLEKDGVK 

       850        860        870        880        890        900 
LLNTWLEGER SITFCRFLSQ NTAKLKLDEI PNAETWPFLV KLLLQCVSME VSGSKRRMPK 

       910        920        930        940        950        960 
PTFAKTLRVV VQRTEETKFP GVQFPLLSMA KTLFTHVHDI LSNTPSFQSE YGTILRHLLE 

       970        980        990       1000       1010       1020 
IKEYRFQMRK RTYSSLVLLY MERAETGFCE KNSGQHSQKE EAFRYILTLQ SLLENSPGDF 

      1030       1040       1050       1060       1070       1080 
PDDLREEIVN GLIHIFSSVR DEGKLSRKLI ECVNTFLLKD GPNLGSLSLE IHNAVEQFVF 

      1090       1100       1110       1120       1130       1140 
RCWLTTHDKN LKEILVSYGR LQLNLTRDSS ESSSLVEQLL DVVTRELDLG SSSSSASWGD 

      1150       1160       1170       1180       1190       1200 
TTKDEKLGAL SSYQNSLVEL AAHVFYRACV NTSRPSLSEK RARRQHIAMR MVDALTEGKW 

      1210       1220       1230       1240       1250       1260 
LWCAAFGCLV RNYCARINMD LLIYWFEAIC TNFQRLLEDA SMRRSYDGLL WTLRSLQGLS 

      1270       1280       1290       1300       1310       1320 
SGLSLPDITM DISKSSASSS ELDRGWQSIW SSLIHGLATF SSMSVIVDAV LVLLGSIISS 

      1330       1340       1350       1360       1370       1380 
NHITVKILPQ EVWDHQLFRH IPSEPALYFI ACYFSRMGCQ GNLQDDLHLR RNLLRAVCAP 

      1390       1400       1410       1420       1430       1440 
LSWKVRLTLD ERMVQLLPAA AFSLCAGFKV SLPLPKEHLP TPSQWDVCEQ IDDVDRERNF 

      1450       1460       1470       1480       1490       1500 
GLFECSVEAL TRICSNSSKI SGCQVPDVVQ LPLVLRDPLL HDMDIYFLSI IPEVKEKGPL 

      1510       1520       1530       1540       1550       1560 
SDIFMGCALL CHFMHGSYIT RKGKGSSSFF LKACQYLLEG LDHAVESVSK SLNDLQRRGS 

      1570       1580       1590       1600       1610       1620 
LGFGSDFNEK GSIIVSLRSF TQSPVFSNRR DQNLLGASYD FVIHSLENLL RSFAKVYEEY 

      1630       1640       1650       1660       1670       1680 
TEHAWNTHSD TVPSKSLAPD SPEVGRIVDM DLDLAEDTKE RDIIAAGGKA VPGLPVSMGN 

      1690       1700       1710       1720       1730       1740 
WKLGMVSLIS CFSPVLQFPT WDVLYNLLEK ESDPKVLENI LYHLCKLSCL TSIPKVDDLV 

      1750       1760       1770       1780       1790       1800 
IFLDGMLSTQ VKMKRNCLNI VTALHVLLHT LSSSRRDSSG VEKNCGLSLK EAESFQVFVQ 

      1810       1820       1830       1840       1850       1860 
LGAMVNKVSE FGLLGWFGRV KLINCICDLV LLNPQTGQTM IERLLLMLSD SDYRVRFVLA 

      1870       1880       1890       1900       1910       1920 
RQIGILFQTW DGHEALFQDI CSSFGIKLVT SSKEKLVTAK DVLAVGPQPR QKMETVIITL 

      1930       1940       1950       1960       1970       1980 
MHLAYHSENI ELQAVFMMCA VSAKDPCQRE LIIAALDNLS AQLHYPSRFK YLEELLGPIL 

      1990       2000       2010       2020       2030       2040 
FHWIASGVSL AGLIETSQLF IPNAEPKYFI HFCSHWLLPA LLLHEDHTNL DWVAKMAGQP 

      2050       2060       2070       2080       2090       2100 
VVVLVKENFV PIFSICMGLH CSKTSECDKG AMVLQNSILY VGETSENERD KLIKQNMVSI 

      2110       2120       2130       2140       2150       2160 
VSFILSCASS SPEPPVPTFS RDTISLAVQT VVDGFLENTD YPKNAAITDR INIFRPDRVF 

      2170       2180       2190       2200       2210       2220 
MFITEMHYRM SAACHHRHTR HHLAALEELT ILLGHRALVP SSLNYIFNLV GQFIGYPSLQ 

      2230       2240       2250       2260       2270       2280 
DQCCSIASCL LDLFKSNPAK EIVSVLGDQL QFLVSKLVTC CIDAEADTKI SGAKSSQLVN 

      2290       2300       2310       2320       2330       2340 
LLHKLVVSSD SSLNEDIRDL EPLPDLKYFQ VIRESHIRIC EAYSPRNHLL KVEHSTFLIY 

      2350       2360       2370       2380       2390       2400 
IFLEILSLSN FLFLSCSTIQ QCSRRSNYLP PRFLSRSLQA LHNKLIASEV SQEDTNGETA 

      2410       2420       2430       2440       2450       2460 
ETFWQSDDEI VNAVWTLVRV SASDEADSMR LLVSDFLSRI GIRDPHTVVF HLPGNLVSMH 

      2470       2480       2490       2500       2510       2520 
GLQGFGHNTG SKVRSLTENG ISDETLITLL NFLKKYLLDD SVKIIDVTSQ TLRGILSTER 

      2530       2540       2550       2560       2570       2580 
GQQALSSFDS CERALIEVHG RGVNLDIVEK ILLDSQKQFK AEKFSLETPE VWSTDNKNFD 

      2590       2600       2610       2620       2630       2640 
RWICQLVYCM IALCEDVPIR LCQNIALLKA EISELLFPSV VVSLAGRIGM DINLHDLITS 

      2650       2660       2670       2680       2690       2700 
QVKEHIFTDS NKLTKSKQVM LNTLNELRMC YVLERSIFSG QTKREKNSRS CSTAAKIRDV 

      2710       2720       2730       2740       2750       2760 
ESGSNGMAAS ITTNWEKVYW LSIDYLVVAG SAVVCGAYLT ASMYVEYWCE EKFGNLSLGD 

      2770       2780       2790       2800       2810       2820 
PDFSYHDKLP DHVEILVSAI TRINEPDSLY GVIHSNKLSA QIITFEHEGN WTRALEYYDL 

      2830       2840       2850       2860       2870       2880 
QARSQKMVVP SSLSENLEVE QFQPTTSARH SVFGEGEVQR QPFKGLIRSL QQTGCMHVLD 

      2890       2900       2910       2920       2930       2940 
LYCRGLTSRE GCFQYDPEFI ELQYEAAWRA GKWDFSLLYP QTHCQPLQHA KNNNYHESLH 

      2950       2960       2970       2980       2990       3000 
CCLRALQEGD YDGFYGKLKD TKKELVLSIS RASEESTEFI YSTVVKLQIL HHLGLVWDLR 

      3010       3020       3030       3040       3050       3060 
WTTSSHQSVH GYLVKQMACV DPVIPTMDQL SWLNKDWNSI ITQTQLHMTL LEPFIAFRRV 

      3070       3080       3090       3100       3110       3120 
LLQILGCEKC TMQHLLQSAS LLRKGTRFSH AAASLHEFKF LCARSNGQQP VPDWLGKLEE 

      3130       3140       3150       3160       3170       3180 
AKLLHAQGRH EVSISLANYI LHNYQLKEEA SDIYRVIGKW LAETRSSNSR TILEKYLRPA 

      3190       3200       3210       3220       3230       3240 
VSLAEEQSSK ICKRLVDRQS QTWFHLAHYA DALFKSYEER LSSSEWQAAL RLRKHKTKEL 

      3250       3260       3270       3280       3290       3300 
EVFIKRFKSS KKAEQSDYSL KIQDLQKQLT MDKEEAEKLQ VDRDNFLKLA LEGYKRCLEI 

      3310       3320       3330       3340       3350       3360 
GDKYDVRVVF RQVSMWFSLA SQKNVIDNML STIKEVQSYK FIPLVYQIAS RLGSSKDESG 

      3370       3380       3390       3400       3410       3420 
SNSFQSALVS LIRKMAIDHP YHTILQLLAL ANGDRIKDNQ RSRNSFVVDM DKKLAAEHLL 

      3430       3440       3450       3460       3470       3480 
QDVSHYHGPM IRQMKQLVDI YIKLAELETR REDTNRKVAL PREIRSVKQL ELVPVVTATI 

      3490       3500       3510       3520       3530       3540 
PVDRSCQYNE GSFPFFRGLS DSVTVMNGIN APKVVECFGS DGQKYKQLAK SGNDDLRQDA 

      3550       3560       3570       3580       3590       3600 
VMEQFFGLVN TFLHNNRDTW KRRLAVRTYK VIPFTPSAGV LEWVDGTIPL GDYLIGSSRS 

      3610       3620       3630       3640       3650       3660 
EGAHGRYGIG NWKYPKCREH MSSAKDKRKA FVDVCTNFRP VMHYFFLEKF LQPADWFVKR 

      3670       3680       3690       3700       3710       3720 
LAYTRSVAAS SMVGYIVGLG DRHAMNILID QATAEVVHID LGVAFEQGLM LKTPERVPFR 

      3730       3740       3750       3760       3770       3780 
LTRDIIDGMG ITGVEGVFRR CCEETLSVMR TNKEALLTIV EVFIHDPLYK WALSPLKALQ 

      3790       3800       3810       3820       3830       3840 
RQKETEDYDG MNLEGLQEEF EGNKDATRAL MRVKQKLDGY EGGEMRSIHG QAQQLIQDAI 

      3850 
DTDRLSHMFP GWGAWM

Q9M3G7 in FASTA format

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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools