ID   AFB2_ARATH              Reviewed;         575 AA.
AC   Q9LW29;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-NOV-2008, entry version 42.
DE   RecName: Full=Protein AUXIN SIGNALING F-BOX 2;
GN   Name=AFB2; Synonyms=LRF2; OrderedLocusNames=At3g26810;
GN   ORFNames=MDJ14.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=20277480; PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   LEUCINE-RICH REPEATS.
RX   MEDLINE=22002558; PubMed=12008900; DOI=10.1023/A:1014440531842;
RA   Thelander M., Fredriksson D., Schouten J., Hoge J.H.C., Ronne H.;
RT   "Cloning by pathway activation in yeast: identification of an
RT   Arabidopsis thaliana F-box protein that can turn on glucose
RT   repression.";
RL   Plant Mol. Biol. 49:69-79(2002).
RN   [4]
RP   FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, AND INTERACTION
RP   WITH IAA7.
RX   PubMed=15992545; DOI=10.1016/j.devcel.2005.05.014;
RA   Dharmasiri N., Dharmasiri S., Weijers D., Lechner E., Yamada M.,
RA   Hobbie L., Ehrismann J.S., Juergens G., Estelle M.;
RT   "Plant development is regulated by a family of auxin receptor F box
RT   proteins.";
RL   Dev. Cell 9:109-119(2005).
RN   [5]
RP   FUNCTION.
RX   PubMed=15917797; DOI=10.1038/nature03543;
RA   Dharmasiri N., Dharmasiri S., Estelle M.;
RT   "The F-box protein TIR1 is an auxin receptor.";
RL   Nature 435:441-445(2005).
RN   [6]
RP   FUNCTION, AND INDUCTION.
RX   PubMed=16627744; DOI=10.1126/science.1126088;
RA   Navarro L., Dunoyer P., Jay F., Arnold B., Dharmasiri N., Estelle M.,
RA   Voinnet O., Jones J.D.G.;
RT   "A plant miRNA contributes to antibacterial resistance by repressing
RT   auxin signaling.";
RL   Science 312:436-439(2006).
CC   -!- FUNCTION: Confers sensitivity to the virulent bacterial pathogen
CC       P.syringae (By similarity). Auxin receptor that mediates Aux/IAA
CC       proteins proteasomal degradation and auxin-regulated
CC       transcription. Involved in embryogenesis regulation by auxin.
CC   -!- SUBUNIT: Interacts with Aux/IAA proteins (IAA7) in an auxin-
CC       dependent manner.
CC   -!- INTERACTION:
CC       Q38825:IAA7; NbExp=1; IntAct=EBI-617556, EBI-602959;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- TISSUE SPECIFICITY: Ubiquitous, with higer levels in seedlings.
CC   -!- INDUCTION: Repressed by miR393a (microRNA) in response to flg-22
CC       (flagellin-derived peptide 22).
CC   -!- SIMILARITY: Contains 1 F-box domain.
CC   -!- SIMILARITY: Contains 16 LRR (leucine-rich) repeats.
CC   -!- WEB RESOURCE: Name=PlantsUBQ; Note=A functional genomics database
CC       for the ubiquitin/26S proteasome proteolytic pathway in plants;
CC       URL="http://plantsubq.genomics.purdue.edu/";
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DR   EMBL; AB016889; BAB01228.1; -; Genomic_DNA.
DR   EMBL; AY062568; AAL32646.1; -; mRNA.
DR   EMBL; BT010396; AAQ56839.1; -; mRNA.
DR   RefSeq; NP_566800.1; -.
DR   UniGene; At.6031; -.
DR   IntAct; Q9LW29; -.
DR   GeneID; 822296; -.
DR   GenomeReviews; BA000014_GR; AT3G26810.
DR   KEGG; ath:AT3G26810; -.
DR   NMPDR; fig|3702.1.peg.14963; -.
DR   TAIR; At3g26810; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0009734; P:auxin mediated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
DR   InterPro; IPR001810; F-box.
DR   SMART; SM00256; FBOX; 1.
DR   PROSITE; PS50181; FBOX; FALSE_NEG.
PE   1: Evidence at protein level;
KW   Auxin signaling pathway; Complete proteome; Developmental protein;
KW   Leucine-rich repeat; Nucleus; Plant defense; Repeat.
FT   CHAIN         1    575       Protein AUXIN SIGNALING F-BOX 2.
FT                                /FTId=PRO_0000272243.
FT   DOMAIN        1     47       F-box.
FT   REPEAT       91    108       LRR 1.
FT   REPEAT      109    141       LRR 2.
FT   REPEAT      142    169       LRR 3.
FT   REPEAT      170    196       LRR 4.
FT   REPEAT      197    223       LRR 5.
FT   REPEAT      224    250       LRR 6.
FT   REPEAT      251    274       LRR 7.
FT   REPEAT      275    300       LRR 8.
FT   REPEAT      301    324       LRR 9.
FT   REPEAT      325    357       LRR 10.
FT   REPEAT      358    382       LRR 11.
FT   REPEAT      383    417       LRR 12.
FT   REPEAT      418    444       LRR 13.
FT   REPEAT      445    466       LRR 14.
FT   REPEAT      467    488       LRR 15.
FT   REPEAT      489    512       LRR 16.
SQ   SEQUENCE   575 AA;  64605 MW;  D383C4E0A76D50D6 CRC64;
     MNYFPDEVIE HVFDFVTSHK DRNAISLVCK SWYKIERYSR QKVFIGNCYA INPERLLRRF
     PCLKSLTLKG KPHFADFNLV PHEWGGFVLP WIEALARSRV GLEELRLKRM VVTDESLELL
     SRSFVNFKSL VLVSCEGFTT DGLASIAANC RHLRDLDLQE NEIDDHRGQW LSCFPDTCTT
     LVTLNFACLE GETNLVALER LVARSPNLKS LKLNRAVPLD ALARLMACAP QIVDLGVGSY
     ENDPDSESYL KLMAVIKKCT SLRSLSGFLE AAPHCLSAFH PICHNLTSLN LSYAAEIHGS
     HLIKLIQHCK KLQRLWILDS IGDKGLEVVA STCKELQELR VFPSDLLGGG NTAVTEEGLV
     AISAGCPKLH SILYFCQQMT NAALVTVAKN CPNFIRFRLC ILEPNKPDHV TSQPLDEGFG
     AIVKACKSLR RLSLSGLLTD QVFLYIGMYA NQLEMLSIAF AGDTDKGMLY VLNGCKKMKK
     LEIRDSPFGD TALLADVSKY ETMRSLWMSS CEVTLSGCKR LAEKAPWLNV EIINENDNNR
     MEENGHEGRQ KVDKLYLYRT VVGTRMDAPP FVWIL
//