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UniProtKB/Swiss-Prot entry Q9LRM7


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NCED6_ARATH
Primary accession number Q9LRM7
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 2007
Sequence was last modified on October 1, 2000 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 31)
Name and origin of the protein
Protein name 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Precursor]
Synonyms AtNCED6
EC 1.13.11.51
Gene name
Name: NCED6
OrderedLocusNames: At3g24220
ORFNames: MUJ8.12
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.2.131; PubMed=10819329 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:131-135(2000).
[2]
FUNCTION, AND INDUCTION BY DROUGHT STRESS.
PubMed=11532178 [NCBI, ExPASy, EBI, Israel, Japan]
Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.;
"Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.";
Plant J. 27:325-333(2001).
[3]
SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY DROUGHT STRESS.
DOI=10.1046/j.1365-313X.2003.01786.x; PubMed=12834401 [NCBI, ExPASy, EBI, Israel, Japan]
Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K., McCarty D.R.;
"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family.";
Plant J. 35:44-56(2003).
[4]
FUNCTION, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
DOI=10.1111/j.1365-313X.2005.02622.x; PubMed=16412079 [NCBI, ExPASy, EBI, Israel, Japan]
Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E., Marion-Poll A.;
"Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy.";
Plant J. 45:309-319(2006).
Comments
  • FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Contributes probably to abscisic acid synthesis for the induction of seed dormancy.
  • CATALYTIC ACTIVITY: A 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal.
  • CATALYTIC ACTIVITY: 9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al.
  • CATALYTIC ACTIVITY: 9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al.
  • SUBCELLULAR LOCATION: Plastid, chloroplast stroma. Note=Partially bound to the thylakoid.
  • TISSUE SPECIFICITY: Expressed before fertilization in male and female gametophytes, and then immediately after pollination, restricted to seed endosperm.
  • DEVELOPMENTAL STAGE: Expressed in seeds at early and mid-maturation stages.
  • INDUCTION: Low induction by drought stress.
  • MISCELLANEOUS: Loss-of-function mutants (T-DNA insertion) exhibit abscisic-acid-deficient phenotypes in seeds, but not in vegetative tissues.
  • SIMILARITY: Belongs to the carotenoid oxygenase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB028621; BAB01363.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_189064.1; -.
UniGene At.37572
3D structure databases
ModBase Q9LRM7.
Organism-specific databases
TAIR At3g24220; -.
Ontologies
GO
GO:0009507; Cellular component: chloroplast (inferred from electronic annotation from UniProtKB-KW).
GO:0045549; Molecular function: 9-cis-epoxycarotenoid dioxygenase activity (inferred from electronic annotation from EC).
GO:0009688; Biological process: abscisic acid biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR004294; Carotenoid_Oase.
Graphical view of domain structure.
PANTHER PTHR10543; Carotenoid_Oase; 1.
Pfam PF03055; RPE65; 1.
Pfam graphical view of domain structure.
ProtoNet Q9LRM7.
Genome annotation databases
GeneID 822008; -.
GenomeReviews BA000014_GR; AT3G24220.
KEGG ath:AT3G24220; -.
NMPDR fig|3702.1.peg.14682; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Abscisic acid biosynthesis; Chloroplast; Complete proteome; Dioxygenase; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
TRANSIT   1     ?        Chloroplast (Potential). 
CHAIN   ?   577        9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic. PRO_0000285995
Sequence information
Length: 577 AA [This is the length of the unprocessed precursor] Molecular weight: 63821 Da [This is the MW of the unprocessed precursor] CRC64: 515E0A1B321B4FE1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MQHSLRSDLL PTKTSPRSHL LPQPKNANIS RRILINPFKI PTLPDLTSPV PSPVKLKPTY 

        70         80         90        100        110        120 
PNLNLLQKLA ATMLDKIESS IVIPMEQNRP LPKPTDPAVQ LSGNFAPVNE CPVQNGLEVV 

       130        140        150        160        170        180 
GQIPSCLKGV YIRNGANPMF PPLAGHHLFD GDGMIHAVSI GFDNQVSYSC RYTKTNRLVQ 

       190        200        210        220        230        240 
ETALGRSVFP KPIGELHGHS GLARLALFTA RAGIGLVDGT RGMGVANAGV VFFNGRLLAM 

       250        260        270        280        290        300 
SEDDLPYQVK IDGQGDLETI GRFGFDDQID SSVIAHPKVD ATTGDLHTLS YNVLKKPHLR 

       310        320        330        340        350        360 
YLKFNTCGKK TRDVEITLPE PTMIHDFAIT ENFVVIPDQQ MVFKLSEMIR GGSPVIYVKE 

       370        380        390        400        410        420 
KMARFGVLSK QDLTGSDINW VDVPDCFCFH LWNAWEERTE EGDPVIVVIG SCMSPPDTIF 

       430        440        450        460        470        480 
SESGEPTRVE LSEIRLNMRT KESNRKVIVT GVNLEAGHIN RSYVGRKSQF VYIAIADPWP 

       490        500        510        520        530        540 
KCSGIAKVDI QNGTVSEFNY GPSRFGGEPC FVPEGEGEED KGYVMGFVRD EEKDESEFVV 

       550        560        570 
VDATDMKQVA AVRLPERVPY GFHGTFVSEN QLKEQVF 

Q9LRM7 in FASTA format

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