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UniProtKB/Swiss-Prot entry Q9LF79


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ACA8_ARATH
Primary accession number Q9LF79
Secondary accession number Q9LU75
Integrated into Swiss-Prot on January 11, 2001
Sequence was last modified on October 1, 2000 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 79)
Name and origin of the protein
Protein name Calcium-transporting ATPase 8, plasma membrane-type
Synonyms EC 3.6.3.8
Ca(2+)-ATPase isoform 8
Gene name
Name: ACA8
OrderedLocusNames: At5g57110
ORFNames: MUL3.5
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND CALMODULIN-BINDING DOMAIN.
STRAIN=cv. Landsberg erecta;
DOI=10.1104/pp.123.4.1495; PubMed=10938365 [NCBI, ExPASy, EBI, Israel, Japan]
Bonza M.C., Morandini P., Luoni L., Geisler M., Palmgren M.G., De Michelis M.I.;
"At-ACA8 encodes a plasma membrane-localized Ca2+-ATPase of Arabidopsis with a calmodulin-binding domain at the N-terminus.";
Plant Physiol. 123:1495-1506(2000).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.1.31; PubMed=10718197 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:31-63(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-19; SER-22; SER-27 AND SER-29, AND MASS SPECTROMETRY.
DOI=10.1105/tpc.104.023150; PubMed=15308754 [NCBI, ExPASy, EBI, Israel, Japan]
Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
"Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database.";
Plant Cell 16:2394-2405(2004).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700164-MCP200; PubMed=17586839 [NCBI, ExPASy, EBI, Israel, Japan]
Niittylae T., Fuglsang A.T., Palmgren M.G., Frommer W.B., Schulze W.X.;
"Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis.";
Mol. Cell. Proteomics 6:1711-1726(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ249352; CAB96189.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB023042; BAA97361.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY069869; AAL47426.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T52654; T52654.
RefSeq NP_200521.3; -.
NP_851200.1; -.
UniGene At.9676
3D structure databases
HSSP P04191; 1EUL. [HSSP ENTRY / PDB]
ModBase Q9LF79.
Protein-protein interaction databases
IntAct Q9LF79; -.
Organism-specific databases
TAIR At5g57110; -.
Gene expression databases
ArrayExpress Q9LF79; -.
GermOnline AT5G57110; Arabidopsis thaliana.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from InterPro).
GO:0005388; Molecular function: calcium-transporting ATPase activity (inferred from electronic annotation from InterPro).
GO:0005516; Molecular function: calmodulin binding (inferred from electronic annotation from UniProtKB-KW).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0006816; Biological process: calcium ion transport (inferred from electronic annotation from InterPro).
GO:0008152; Biological process: metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR006408; ATPase-IIB_Ca.
IPR001757; ATPase_P.
IPR006068; ATPase_P_cat_C.
IPR004014; ATPase_P_cat_N.
IPR005834; Dehalogen-like_hydro.
IPR008250; E1-E2_ATPase_reg.
Graphical view of domain structure.
PANTHER PTHR11939; ATPase_P; 1.
Pfam PF00689; Cation_ATPase_C; 1.
PF00690; Cation_ATPase_N; 1.
PF00122; E1-E2_ATPase; 1.
PF00702; Hydrolase; 1.
Pfam graphical view of domain structure.
PRINTS PR00119; CATATPASE.
TIGRFAMs TIGR01517; ATPase-IIB_Ca; 1.
TIGR01494; ATPase_P-type; 3.
PROSITE PS00154; ATPASE_E1_E2; 1.
ProtoNet Q9LF79.
Genome annotation databases
GeneID 835815; -.
GenomeReviews BA000015_GR; AT5G57110.
KEGG ath:AT5G57110; -.
NMPDR fig|3702.1.peg.27655; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Calcium; Calcium transport; Calmodulin-binding; Cell membrane; Complete proteome; Direct protein sequencing; Hydrolase; Ion transport; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1074  1074     Calcium-transporting ATPase 8, plasma membrane-type. PRO_0000046414
TOPO_DOM   1    180  180     Cytoplasmic (Potential). 
TRANSMEM   181    201  21     Potential. 
TOPO_DOM   202    219  18     Extracellular (Potential). 
TRANSMEM   220    240  21     Potential. 
TOPO_DOM   241    369  129     Cytoplasmic (Potential). 
TRANSMEM   370    389  20     Potential. 
TOPO_DOM   390    426  37     Extracellular (Potential). 
TRANSMEM   427    444  18     Potential. 
TOPO_DOM   445    840  396     Cytoplasmic (Potential). 
TRANSMEM   841    859  19     Potential. 
TOPO_DOM   860    870  11     Extracellular (Potential). 
TRANSMEM   871    891  21     Potential. 
TOPO_DOM   892    911  20     Cytoplasmic (Potential). 
TRANSMEM   912    934  23     Potential. 
TOPO_DOM   935    949  15     Extracellular (Potential). 
TRANSMEM   950    971  22     Potential. 
TOPO_DOM   972    989  18     Cytoplasmic (Potential). 
TRANSMEM   990   1011  22     Potential. 
TOPO_DOM   1012   1021  10     Extracellular (Potential). 
TRANSMEM   1022   1043  22     Potential. 
TOPO_DOM   1044   1074  31     Cytoplasmic (Potential). 
REGION   43     54  12     Interaction with calmodulin. 
ACT_SITE   482    482        4-aspartylphosphate intermediate (By similarity). 
METAL   785    785        Magnesium (By similarity). 
METAL   789    789        Magnesium (By similarity). 
MOD_RES   19     19        Phosphoserine. 
MOD_RES   22     22        Phosphoserine. 
MOD_RES   27     27        Phosphoserine. 
MOD_RES   29     29        Phosphoserine. 
Sequence information
Length: 1074 AA [This is the length of the unprocessed precursor] Molecular weight: 116174 Da [This is the MW of the unprocessed precursor] CRC64: 2E86572F44DEA8A5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTSLLKSSPG RRRGGDVESG KSEHADSDSD TFYIPSKNAS IERLQQWRKA ALVLNASRRF 

        70         80         90        100        110        120 
RYTLDLKKEQ ETREMRQKIR SHAHALLAAN RFMDMGRESG VEKTTGPATP AGDFGITPEQ 

       130        140        150        160        170        180 
LVIMSKDHNS GALEQYGGTQ GLANLLKTNP EKGISGDDDD LLKRKTIYGS NTYPRKKGKG 

       190        200        210        220        230        240 
FLRFLWDACH DLTLIILMVA AVASLALGIK TEGIKEGWYD GGSIAFAVIL VIVVTAVSDY 

       250        260        270        280        290        300 
KQSLQFQNLN DEKRNIHLEV LRGGRRVEIS IYDIVVGDVI PLNIGNQVPA DGVLISGHSL 

       310        320        330        340        350        360 
ALDESSMTGE SKIVNKDANK DPFLMSGCKV ADGNGSMLVT GVGVNTEWGL LMASISEDNG 

       370        380        390        400        410        420 
EETPLQVRLN GVATFIGSIG LAVAAAVLVI LLTRYFTGHT KDNNGGPQFV KGKTKVGHVI 

       430        440        450        460        470        480 
DDVVKVLTVA VTIVVVAVPE GLPLAVTLTL AYSMRKMMAD KALVRRLSAC ETMGSATTIC 

       490        500        510        520        530        540 
SDKTGTLTLN QMTVVESYAG GKKTDTEQLP ATITSLVVEG ISQNTTGSIF VPEGGGDLEY 

       550        560        570        580        590        600 
SGSPTEKAIL GWGVKLGMNF ETARSQSSIL HAFPFNSEKK RGGVAVKTAD GEVHVHWKGA 

       610        620        630        640        650        660 
SEIVLASCRS YIDEDGNVAP MTDDKASFFK NGINDMAGRT LRCVALAFRT YEAEKVPTGE 

       670        680        690        700        710        720 
ELSKWVLPED DLILLAIVGI KDPCRPGVKD SVVLCQNAGV KVRMVTGDNV QTARAIALEC 

       730        740        750        760        770        780 
GILSSDADLS EPTLIEGKSF REMTDAERDK ISDKISVMGR SSPNDKLLLV QSLRRQGHVV 

       790        800        810        820        830        840 
AVTGDGTNDA PALHEADIGL AMGIAGTEVA KESSDIIILD DNFASVVKVV RWGRSVYANI 

       850        860        870        880        890        900 
QKFIQFQLTV NVAALVINVV AAISSGDVPL TAVQLLWVNL IMDTLGALAL ATEPPTDHLM 

       910        920        930        940        950        960 
GRPPVGRKEP LITNIMWRNL LIQAIYQVSV LLTLNFRGIS ILGLEHEVHE HATRVKNTII 

       970        980        990       1000       1010       1020 
FNAFVLCQAF NEFNARKPDE KNIFKGVIKN RLFMGIIVIT LVLQVIIVEF LGKFASTTKL 

      1030       1040       1050       1060       1070 
NWKQWLICVG IGVISWPLAL VGKFIPVPAA PISNKLKVLK FWGKKKNSSG EGSL 

Q9LF79 in FASTA format

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