NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
Levesque R.C., Liao X., Lightfoot J., Charlebois I., Ouellet C., Morency M., Dewar K., Siehnel R., Lam J., Hancock R.E.;
"Physical mapping of 38 loci including aimE, ampC, ampR, arcA, aroK, catR, cypH, dapB, envA, envC, ftsA, ftsZ, groEL, murE, opdE, oprD, oprF, oprH, oprI, oprK, oprP, pbpB, pbpC, pheS, phoA, phoB, phoS, ponA, pyoS1, qin, rpoB, rpoH, sodB, soxR, sucC.";
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
DOI=10.1038/35023079; PubMed=10984043 [NCBI, ExPASy, EBI, Israel, Japan]
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen.";
Nature 406:959-964(2000).

Comments

FUNCTION: Cell wall formation (By similarity).
CATALYTIC ACTIVITY: ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
COFACTOR: Binds 2 magnesium or manganese ions per subunit (By similarity).
PATHWAY: Cell wall biosynthesis; peptidoglycan biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
SIMILARITY: Contains 1 ATP-grasp domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U19797; AAF26456.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE004091; AAG07798.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

C83094; C83094.

RefSeq

NP_253100.1; -.

3D structure databases

HSSP

P07862; 1IOW. [HSSP ENTRY / PDB]

ModBase

Q9LCT6.

Enzyme and pathway databases

BioCyc

PAER208964:PA4410-MON; -.

Organism-specific databases

PseudoCAP

PA4410; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0008716;	Molecular function: D-alanine-D-alanine ligase activity (inferred from electronic annotation from HAMAP).
GO:0009252;	Biological process: peptidoglycan biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00047; -; 1.
PBIL [Tree]

InterPro

IPR011761; ATP-grasp.
IPR013816; ATP_grasp_subdomain_2.
IPR000291; D-Ala_lig_Van_CS.
IPR005905; D_ala_D_ala.
IPR011095; Dala_Dala_lig_C.
IPR011127; Dala_Dala_lig_N.
IPR013817; Pre-ATP_grasp.
Graphical view of domain structure.

Gene3D

G3DSA:3.30.470.20; ATP_grasp_subdomain_2; 1.
G3DSA:3.40.50.20; Pre-ATP_grasp; 1.

Pfam

PF07478; Dala_Dala_lig_C; 1.
PF01820; Dala_Dala_lig_N; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01205; D_ala_D_alaTIGR; 1.

PROSITE

PS50975; ATP_GRASP; 1.
PS00843; DALA_DALA_LIGASE_1; 1.
PS00844; DALA_DALA_LIGASE_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9LCT6.

Genome annotation databases

881282; -.

AE004091_GR; PA4410.

pae:PA4410; -.

fig|208964.1.peg.4410; -.

Phylogenomic databases

HOGENOM

Q9LCT6; -.

Genome annotation databases

CMR

Q9LCT6; PA4410.

Other

ProtoNet

Q9LCT6.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Cytoplasm; Ligase; Magnesium; Manganese; Metal-binding; Nucleotide-binding; Peptidoglycan synthesis.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 319`	`319`	`D-alanine--D-alanine ligase B.`	`PRO_0000177856`
`DOMAIN`	`117 312`	`196`	`ATP-grasp.`
`NP_BIND`	`143 198`	`56`	`ATP (By similarity).`
`METAL`	`266 266`		`Magnesium or manganese 1 (By similarity).`
`METAL`	`279 279`		`Magnesium or manganese 1 (By similarity).`
`METAL`	`279 279`		`Magnesium or manganese 2 (By similarity).`
`METAL`	`281 281`		`Magnesium or manganese 2 (By similarity).`

Sequence information

Length: 319 AA [This is the length of the unprocessed precursor]

Molecular weight: 34363 Da [This is the MW of the unprocessed precursor]

CRC64: 9470EF555A745419 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNLCLDSLLN GTQDPKAFGR VAVLFGGKSA EREVSLKSGA MVLQSLLAAG VDAFGIDVGE 

        70         80         90        100        110        120 
DLLQRLVEEK IDRAFIILHG RGGEDGSMQG LLECAGIPYT GSGVLASALA MDKLRTKRVW 

       130        140        150        160        170        180 
LSLGLPTPDY AVLASEDDCR EAAQRLGFPL IVKPAHEGSS IGMAKVGGLD ELIAAWREAA 

       190        200        210        220        230        240 
RYDSQVLVEQ WISGPEFTVA TLRGQVLPAI RLGTPHTFYD YDAKYLASDT RYQVPCGLDE 

       250        260        270        280        290        300 
AKERELKELT ARACDALGIQ GWGRADVMQD AEGRFWLLEV NTAPGMTDHS LVPMAARAAG 

       310 
LDFQQLVLAI LADSREARG

Q9LCT6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools