NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
STRAIN=ICR;
DOI=10.1016/S0925-4773(00)00369-5; PubMed=10940631 [NCBI, ExPASy, EBI, Israel, Japan]
Yin Y., Sanes J.R., Miner J.H.;
"Identification and expression of mouse netrin-4.";
Mech. Dev. 96:115-119(2000).

[2]

NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
STRAIN=BALB/c;
DOI=10.1083/jcb.151.2.221; PubMed=11038171 [NCBI, ExPASy, EBI, Israel, Japan]
Koch M., Murrell J.R., Hunter D.D., Olson P.F., Jin W., Keene D.R., Brunken W.J., Burgeson R.E.;
"A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin. Identification, expression, and functional characterization.";
J. Cell Biol. 151:221-234(2000).

Comments

FUNCTION: May play an important role in neural, kidney and vascular development. Promotes neurite elongation from olfactory bulb explants.
SUBUNIT: May form a homodimer.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
TISSUE SPECIFICITY: Expressed in kidney, liver, heart, ovary, testis, retina, brain, olfactory bulb, and widely expressed in embryo.
SIMILARITY: Contains 3 laminin EGF-like domains.
SIMILARITY: Contains 1 laminin N-terminal domain.
SIMILARITY: Contains 1 NTR domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF268066; AAF91404.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF281278; AAG30823.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00119840; -.

UniGene

Mm.448571

3D structure databases

HSSP

P02468; 1NPE. [HSSP ENTRY / PDB]

ModBase

Q9JI33.

Organism-specific databases

MGI

MGI:1888978; Ntn4.

Gene expression databases

Q9JI33; -.

Q9JI33; -.

MM_NTN4; -.

ENSMUSG00000020019; Mus musculus.

Ontologies

GO:0005604;	Cellular component: basement membrane (inferred from direct assay from MGI).
GO:0005615;	Cellular component: extracellular space (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005515;	Molecular function: protein binding (inferred from electronic annotation from InterPro).
GO:0016322;	Biological process: neuron remodeling (inferred from direct assay from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR013032; EGF-like_reg_CS.
IPR002049; EGF_laminin.
IPR008211; Laminin_N.
IPR001134; Netrin_domain.
IPR018933; Netrin_module_non-TIMP.
Graphical view of domain structure.

Pfam

PF00053; Laminin_EGF; 3.
PF00055; Laminin_N; 1.
PF01759; NTR; 1.
Pfam graphical view of domain structure.

PRINTS

PR00011; EGFLAMININ.

SMART

SM00643; C345C; 1.
SM00180; EGF_Lam; 3.
SM00136; LamNT; 1.
SMART graphical view of domain structure.

PROSITE

PS00022; EGF_1; 2.
PS01248; EGF_LAM_1; 2.
PS50027; EGF_LAM_2; 3.
PS51117; LAMININ_NTER; 1.
PS50189; NTR; 1.
PROSITE graphical view of domain structure (profiles).

Proteomic databases

PRIDE

Q9JI33; -.

Genome annotation databases

Ensembl

ENSMUSG00000020019; Mus musculus. [Contig view]

KEGG

mmu:57764; -.

Phylogenomic databases

HOGENOM

Q9JI33; -.

HOVERGEN

Q9JI33; -.

Other

313932; -.

Ntn4; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Basement membrane; Disulfide bond; Extracellular matrix; Glycoprotein; Laminin EGF-like domain; Repeat; Secreted; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 19`	`19`	`Potential.`
`CHAIN`	`20 628`	`609`	`Netrin-4.`	`PRO_0000042117`
`DOMAIN`	`30 261`	`232`	`Laminin N-terminal.`
`DOMAIN`	`262 331`	`70`	`Laminin EGF-like 1.`
`DOMAIN`	`332 394`	`63`	`Laminin EGF-like 2.`
`DOMAIN`	`395 448`	`54`	`Laminin EGF-like 3.`
`DOMAIN`	`506 627`	`122`	`NTR.`
`CARBOHYD`	`56 56`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`163 163`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`353 353`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`483 483`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`262 271`		`By similarity.`
`DISULFID`	`264 293`		`By similarity.`
`DISULFID`	`295 304`		`By similarity.`
`DISULFID`	`307 329`		`By similarity.`
`DISULFID`	`332 341`		`By similarity.`
`DISULFID`	`334 359`		`By similarity.`
`DISULFID`	`362 371`		`By similarity.`
`DISULFID`	`374 392`		`By similarity.`
`DISULFID`	`395 413`		`By similarity.`
`DISULFID`	`397 420`		`By similarity.`
`DISULFID`	`422 431`		`By similarity.`
`DISULFID`	`434 446`		`By similarity.`
`DISULFID`	`506 576`		`By similarity.`
`DISULFID`	`520 627`		`By similarity.`

Sequence information

Length: 628 AA [This is the length of the unprocessed precursor]

Molecular weight: 69897 Da [This is the MW of the unprocessed precursor]

CRC64: 474F747475F236C7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGSCARLLLL WGCSAVAAGL NGVAGANSRC EKACNPRMGN LALGRKLRAD TMCGQNATEL 

        70         80         90        100        110        120 
FCFYSENADL TCRQPKCDKC NAAHSHLAHP PSAMADSSFR FPRTWWQSAE DVHREKIQLD 

       130        140        150        160        170        180 
LEAEFYFTHL IMVFKSPRPA AMVLDRSQDF GKTWKPYKYF ATNCSATFGL EDDVVKKGAI 

       190        200        210        220        230        240 
CTSRYSNPFP CTGGEVIFRA LSPPYDIENP YSAKVQEQLK ITNLRVRLLK RQSCPCQIND 

       250        260        270        280        290        300 
LNAKPHHFMH YAVYDFIVKG SCFCNGHADQ CLPVEGFRPI KAPGAFHVVH GRCMCKHNTA 

       310        320        330        340        350        360 
GSHCQHCAPL YNDRPWEAAD GRTGAPNECR TCKCNGHADT CHFDVNVWEA SGNRSGGVCN 

       370        380        390        400        410        420 
NCQHNTEGQH CQRCKPGFYR DLRRPFSAPD ACKACSCHPV GSAILPFSSV TFCDPSNGDC 

       430        440        450        460        470        480 
PCKPGVAGPH CDRCMVGYWG FGDYGCRPCD CAGSCDPLTG DCISSNADVD WYHEVPTFHS 

       490        500        510        520        530        540 
MHNKSEPSWE WEDEQGFSAL RHSGKCECKE QVLGNPKAFC GMKYSYVLKI KILSAHDKGS 

       550        560        570        580        590        600 
HAEVNVKIKK VLKSTKLKIL RGKRTLYPES WTNRGCTCPI LNPGLEYLVA GHEDVRTGKL 

       610        620 
IVNMKSFVQH WKPALGRRVM HILKRDCV

Q9JI33 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools