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UniProtKB/Swiss-Prot entry Q9JHD1


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PCAF_MOUSE
Primary accession number Q9JHD1
Secondary accession numbers Q3U142 Q640M9
Integrated into Swiss-Prot on March 18, 2008
Sequence was last modified on March 18, 2008 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 48)
Name and origin of the protein
Protein name Histone acetyltransferase PCAF
Synonyms Histone acetylase PCAF
EC 2.3.1.48
P300/CBP-associated factor
P/CAF
Gene name
Name: Pcaf
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9742083 [NCBI, ExPASy, EBI, Israel, Japan]
Xu W., Edmondson D.G., Roth S.Y.;
"Mammalian GCN5 and P/CAF acetyltransferases have homologous amino-terminal domains important for recognition of nucleosomal substrates.";
Mol. Cell. Biol. 18:5659-5669(1998).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=NOD;
TISSUE=Spleen;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6;
TISSUE=Brain, and Eye;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-20, AND MASS SPECTROMETRY.
TISSUE=Liver;
DOI=10.1073/pnas.0609836104; PubMed=17242355 [NCBI, ExPASy, EBI, Israel, Japan]
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
"Large-scale phosphorylation analysis of mouse liver.";
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF254442; AAF70498.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK156290; BAE33658.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC082581; AAH82581.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC145896; AAI45897.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00471164; -.
RefSeq NP_064389.2; -.
UniGene Mm.255025
3D structure databases
HSSP Q92831; 1CM0. [HSSP ENTRY / PDB]
SMR Q9JHD1; 474-634, 696-813.
ModBase Q9JHD1.
Enzyme and pathway databases
BRENDA 2.3.1.48; 244.
Organism-specific databases
MGI MGI:1343094; Pcaf.
Gene expression databases
ArrayExpress Q9JHD1; -.
Bgee Q9JHD1; -.
Ontologies
GO
GO:0016585; Cellular component: chromatin remodeling complex (inferred from sequence or structural similarity from UniProtKB).
GO:0000123; Cellular component: histone acetyltransferase complex (inferred from direct assay from MGI).
GO:0000776; Cellular component: kinetochore (inferred from direct assay from MGI).
GO:0004406; Molecular function: H3/H4 histone acetyltransferase activity (inferred from direct assay from MGI).
GO:0003713; Molecular function: transcription coactivator activity (inferred from direct assay from MGI).
GO:0007049; Biological process: cell cycle (inferred from electronic annotation from UniProtKB-KW).
GO:0016573; Biological process: histone acetylation (inferred from direct assay from MGI).
GO:0045941; Biological process: positive regulation of transcription (inferred from direct assay from MGI).
GO:0006355; Biological process: regulation of transcription, DNA-dependent (traceable author statement from MGI).
GO:0006350; Biological process: transcription (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR016181; Acyl_CoA_acyltransferase.
IPR001487; Bromodomain.
IPR018359; Bromodomain_CS.
IPR000182; GCN5-rel_AcTrfase.
IPR016376; Hist_acetylase_PCAF.
IPR009464; PCAF_N.
Graphical view of domain structure.
Gene3D G3DSA:3.40.630.30; Acyl_CoA_acyltransferase; 1.
G3DSA:1.20.920.10; Bromodomain; 1.
Pfam PF00583; Acetyltransf_1; 1.
PF00439; Bromodomain; 1.
PF06466; PCAF_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF003048; Histone_acetylase_PCAF; 1.
PRINTS PR00503; BROMODOMAIN.
SMART SM00297; BROMO; 1.
SMART graphical view of domain structure.
PROSITE PS00633; BROMODOMAIN_1; 1.
PS50014; BROMODOMAIN_2; 1.
PS51186; GNAT; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE Q9JHD1; -.
Genome annotation databases
Ensembl ENSMUSG00000000708; Mus musculus. [Contig view]
GeneID 18519; -.
KEGG mmu:18519; -.
Phylogenomic databases
HOVERGEN Q9JHD1; -.
OMA Q9JHD1; YKENYTR.
Other
NextBio 294272; -.
SOURCE Pcaf; Mus musculus.
ProtoNet Q9JHD1.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acyltransferase; Bromodomain; Cell cycle; Nucleus; Phosphoprotein; Transcription; Transcription regulation; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   813  813     Histone acetyltransferase PCAF. PRO_0000322986
DOMAIN   484   632  149     N-acetyltransferase. 
DOMAIN   721   791  71     Bromo. 
COMPBIAS   48    53  6     Poly-Gly. 
MOD_RES   20    20        Phosphoserine. 
MOD_RES   710   710        Phosphotyrosine (By similarity). 
CONFLICT   293   294        LL -> FV (in Ref. 1; AAF70498). 
CONFLICT   319   319        E -> G (in Ref. 2; BAE33658). 
CONFLICT   459   459        H -> L (in Ref. 1; AAF70498). 
CONFLICT   548   548        G -> A (in Ref. 1; AAF70498). 
CONFLICT   554   554        F -> L (in Ref. 1; AAF70498). 
Sequence information
Length: 813 AA [This is the length of the unprocessed precursor] Molecular weight: 91769 Da [This is the MW of the unprocessed precursor] CRC64: 38E52F326794F523 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAEAGGAGSP ALPPAPPHGS PRTLATAAGS SASCGPATAV AAAGTAEGPG GGGSARIAVK 

        70         80         90        100        110        120 
KAQLRSAPRA KKLEKLGVYS ACKAEESCKC NGWKNPNPSP TPPRGDLQQI IVSLTESCRS 

       130        140        150        160        170        180 
CSHALAAHVS HLENVSEEEM DRLLGIVLDV EYLFTCVHKE EDADTKQVYF YLFKLLRKSI 

       190        200        210        220        230        240 
LQRGKPVVEG SLEKKPPFEK PSIEQGVNNF VQYKFSHLPS KERQTTIELA KMFLNRINYW 

       250        260        270        280        290        300 
HLEAPSQRRL RSPNDDISGY KENYTRWLCY CNVPQFCDSL PRYETTKVFG RTLLRSVFTI 

       310        320        330        340        350        360 
MRRQLLEQAR QEKDKLPLEK RTLILTHFPK FLSMLEEEVY SQNSPIWDQD FLSASSRTSP 

       370        380        390        400        410        420 
LGIQTVISPP VTGTALFSSN STSHEQINGG RTSPGCRGSS GLEANPGEKR KMNNSHAPEE 

       430        440        450        460        470        480 
AKRSRVMGDI PVELINEVMS TITDPAGMLG PETNFLSAHS ARDEAARLEE RRGVIEFHVV 

       490        500        510        520        530        540 
GNSLNQKPNK KILMWLVGLQ NVFSHQLPRM PKEYITRLVF DPKHKTLALI KDGRVIGGIC 

       550        560        570        580        590        600 
FRMFPSQGFT EIVFCAVTSN EQVKGYGTHL MNHLKEYHIK HEILNFLTYA DEYAIGYFKK 

       610        620        630        640        650        660 
QGFSKEIKIP KTKYVGYIKD YEGATLMGCE LNPQIPYTEF SVIIKKQKEI IKKLIERKQA 

       670        680        690        700        710        720 
QIRKVYPGLS CFKDGVRQIP IESIPGIRET GWKPSGKEKS KEPKDPEQLY STLKNILQQV 

       730        740        750        760        770        780 
KNHPNAWPFM EPVKRTEAPG YYEVIRFPMD LKTMSERLRN RYYVSKKLFM ADLQRVFTNC 

       790        800        810 
KEYNPPESEY YKCASILEKF FFSKIKEAGL IDK 

Q9JHD1 in FASTA format

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