Protein p19
Transmembrane protein 1A
Serine protease p27
     (p27)
     (EC 3.4.21.-)
Protein p20
RNA-directed RNA polymerase p57
     (p57)
     (EC 2.7.7.48)

Gene name

Name:

ORF1

From

Avian nephritis virus 1 (ANV-1)

[TaxID: 336960]

Taxonomy

Viruses; ssRNA positive-strand viruses, no DNA stage; Astroviridae; Avastrovirus.

Virus hosts

Gallus gallus (Chicken) [TaxID: 9031]
Meleagris gallopavo (Common turkey) [TaxID: 9103]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
STRAIN=G-4260;
DOI=10.1128/JVI.74.18.8487-8493.2000; PubMed=10954549 [NCBI, ExPASy, EBI, Israel, Japan]
Imada T., Yamaguchi S., Mase M., Tsukamoto K., Kubo M., Morooka A.;
"Avian nephritis virus (ANV) as a new member of the family Astroviridae and construction of infectious ANV cDNA.";
J. Virol. 74:8487-8493(2000).

Comments

FUNCTION: Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by ribosomal frameshifting.

Name	nsp1ab
Isoform ID	Q9JGF2-1
Note: Generated by a ribosomal frameshift at position 1005.
This is the isoform sequence displayed in this entry.

Name	nsp1a
Isoform ID	P0C6K7-1
This isoform is stored in UniProtKB/Swiss-Prot entry P0C6K7.

PTM: Cleaved presumably by viral and host proteases (By similarity).
SIMILARITY: Belongs to the astroviridae polyprotein 1AB family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB033998; BAA92848.1; ALT_SEQ; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q9JGF2.

Protein family/group databases

MEROPS

S01.109; -.

Family and domain databases

InterPro

IPR008760; Peptidase_S32.
IPR001205; RNA_pol_P3D.
IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.

Pfam

PF05579; Peptidase_S32; 1.
PF00680; RdRP_1; 1.
Pfam graphical view of domain structure.

PROSITE

PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Coiled coil; Hydrolase; Membrane; Nucleotide-binding; Nucleotidyltransferase; Protease; Ribosomal frameshifting; RNA replication; RNA-directed RNA polymerase; Thiol protease; Transferase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1512`	`1512`	`Non-structural polyprotein 1AB.`	`PRO_0000327319`
`CHAIN`	`1 204`	`204`	`Protein p19 (Potential).`	`PRO_0000327320`
`CHAIN`	`205 478`	`274`	`Transmembrane protein 1A (Potential).`	`PRO_0000327321`
`CHAIN`	`479 730`	`252`	`Serine protease p27 (Potential).`	`PRO_0000327322`
`CHAIN`	`731 954`	`224`	`Protein p20 (Potential).`	`PRO_0000327323`
`CHAIN`	`955 1512`	`558`	`RNA-directed RNA polymerase p57 (Potential).`	`PRO_0000327324`
`TRANSMEM`	`190 210`	`21`	`Potential.`
`TRANSMEM`	`295 315`	`21`	`Potential.`
`TRANSMEM`	`323 343`	`21`	`Potential.`
`TRANSMEM`	`360 380`	`21`	`Potential.`
`TRANSMEM`	`399 419`	`21`	`Potential.`
`DOMAIN`	`1257 1390`	`134`	`RdRp catalytic.`
`ACT_SITE`	`524 524`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`556 556`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`621 621`		`Charge relay system; for serine protease activity (By similarity).`
`SITE`	`204 205`	`2`	`Cleavage (Potential).`
`SITE`	`478 479`	`2`	`Cleavage (Potential).`
`SITE`	`730 731`	`2`	`Cleavage (Potential).`
`SITE`	`954 955`	`2`	`Cleavage (Potential).`

Sequence information

Length: 1512 AA [This is the length of the unprocessed precursor]

Molecular weight: 171273 Da [This is the MW of the unprocessed precursor]

CRC64: 195D9D1DC048DCDC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MASAGPTGAG ARPPKAFTAQ AGLAKLVNPA GLNSILARGK EKFGGTQAWK ELMGCDVIFA 

        70         80         90        100        110        120 
RSISHWYGIK GTTYYELTVA LGQPLYKPVT DPELTEEEKA VMTAVQSRFA QSNSSVVLTR 

       130        140        150        160        170        180 
TLLNKTCELK DRIRELTDEL GQTEVHLARE KVKAAALKLE NRKLFVENQE LKDQLEKERT 

       190        200        210        220        230        240 
KHGWKGLKTL CLWIFLATLI GGYITGSNAA CTLVDVPSPM KVGYDTFKQM CIHKDSYLPD 

       250        260        270        280        290        300 
GAFDKESLAL ECSKQMDYMD CKEVITDSIS GKTSFAGMLR DVFRVDEIVT AIRTVVRFAM 

       310        320        330        340        350        360 
DFSLAYPICV MFVLILTRNK KHAIISACCA LVAKCCGLRL LPFTLVLTYA PSETAIAGCI 

       370        380        390        400        410        420 
YGLGYISIPL VTFLHWVGLV LKAILVPDDC YIGTRVSHAL AWSIMLPMWI ITQELMAFTE 

       430        440        450        460        470        480 
FPLELQIVTT VVVCTAGFGF RYLTGTVTIT EPDGTVKKYK RIFNAKSAIG TISTVFFEKA 

       490        500        510        520        530        540 
KAIRGVIPSF PSKADNIVKI EVDVDGGSAG VGFRLGNYIY TAGHVVGEAK IAKITWKGLT 

       550        560        570        580        590        600 
SQAKVLGHIE LPLFTDTLAR LEIPKPFQQL PVFRLAKSSE NDYVQMVCFD NQLQNVVTFS 

       610        620        630        640        650        660 
GWANIDGDYL NAPFETYAGT SGSPIINRDG RMLGVHFGSN AVVSQGFVIT RLFATEPAVK 

       670        680        690        700        710        720 
QCKSDEDLAD EIVRKVMGGI RISFASLTSE LEKQRDELNA LKQMVNDLID TDLVALEKKK 

       730        740        750        760        770        780 
GKTKRTVRGQ KHKTKAISKA AFMKTKVLTE EEYRRLEEEG FTKDEIKDIV DNLREQAWLD 

       790        800        810        820        830        840 
YQNQLDEEGD DDWYEQMEED QRINDQIDQN IERDLEDRGE WYGQRKITFK QRAMLRFIQL 

       850        860        870        880        890        900 
GRQQQVATVS FPDGYEDRAE ELYNKVVTTE DLPEGETSEA ALSLPNKIVH QAGKRLNFKH 

       910        920        930        940        950        960 
VKIHPDKTFM KSGVTQIEEK PEGDIILKAK TTTLAPKEEP VIQQVEQQPQ VEQQQQPQQP 

       970        980        990       1000       1010       1020 
VVEEKKRTPP PKPQRKPKTG AKAKCLDCGE TFVERQDFHV CKSKKLNEPP SGGYTPVPDH 

      1030       1040       1050       1060       1070       1080 
LRWNNWQIYM EPLDLRITVP ENYPILGHIA IDKLVERKKK VNDPLLKMLE QPKCEGFTST 

      1090       1100       1110       1120       1130       1140 
TWTRKAYTKS FEKFDYGDAV DFVQDYPELT AFADAAVLAE VGYMEGTHVI PIQETSKNMD 

      1150       1160       1170       1180       1190       1200 
STPAFPKMLD FDSERDYLEA HGMKEYIDTQ LGVQSGKPLW WCFLKNEILK EKKVSEDDIR 

      1210       1220       1230       1240       1250       1260 
IITCSDPVIT RLGASFDSEQ NERMKERTET HHAQVGWTPF FGGLDKRVRR ITSCGRTQVL 

      1270       1280       1290       1300       1310       1320 
ELDWTRFDGT IPVQLFQRMR ELRKFFLTRR SRRRYGKLLD WYNAQLTDRI TLLPTGEVTH 

      1330       1340       1350       1360       1370       1380 
VKKGNPSGQF STTVDNNLVN EWLTAFEFGY QHLENHGIIP TVRDYRANVD FLCYGDDRLL 

      1390       1400       1410       1420       1430       1440 
AFNPSFVNYD PQVTIDMYKN IFGMWVKPEN IKLFDSPTGS SFCGFTLVKP HGQWVGVVNV 

      1450       1460       1470       1480       1490       1500 
NKLLQSLKTP TRRLPDLESL WGKLVSLKIM CYHSDPEAVS YLSNQIRRVE EYARAEGIEL 

      1510 
PEVGPDFYRK IW

Q9JGF2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools