Protein p19
Transmembrane protein 1A
Serine protease p27
     (p27)
     (EC 3.4.21.-)
Protein p20
RNA-directed RNA polymerase p57
     (p57)
     (EC 2.7.7.48)

Gene name

Name:

ORF1

From

Human astrovirus-8 (HAstV-8)

[TaxID: 43358]

Taxonomy

Viruses; ssRNA positive-strand viruses, no DNA stage; Astroviridae; Mamastrovirus.

Virus host

Homo sapiens (Human) [TaxID: 9606]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate Yuc-8; PubMed=11086120 [NCBI, ExPASy, EBI, Israel, Japan] Mendez-Toss M., Romero-Guido P., Munguia M.E., Mendez E., Arias C.F.; "Molecular analysis of a serotype 8 human astrovirus genome."; J. Gen. Virol. 81:2891-2897(2000).
[2]	NSP1AB POLYPROTEIN PROCESSING. DOI=10.1128/JVI.77.21.11378-11384.2003; PubMed=14557623 [NCBI, ExPASy, EBI, Israel, Japan] Mendez E., Salas-Ocampo M.P., Munguia M.E., Arias C.F.; "Protein products of the open reading frames encoding nonstructural proteins of human astrovirus serotype 8."; J. Virol. 77:11378-11384(2003).

Comments

FUNCTION: Non-structural polyprotein 1AB contains the viral protease participating in the cleavage of the polyprotein into functional products. It contains also the activities necessary for replication of genomic RNA, as well as transcription of subgenomic mRNA.
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by ribosomal frameshifting.

Name	nsp1ab
Isoform ID	Q9IFX2-1
Note: Generated by a ribosomal frameshift at position 900.
This is the isoform sequence displayed in this entry.

Name	nsp1a
Isoform ID	Q9IFX3-1
This isoform is stored in UniProtKB/Swiss-Prot entry Q9IFX3.

PTM: Cleaved presumably by viral and host proteases.
SIMILARITY: Belongs to the astroviridae polyprotein 1AB family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF260508; AAF85963.1; ALT_SEQ; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

C49529; C49529.

3D structure databases

ModBase

Q9IFX2.

Family and domain databases

InterPro

IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.

PROSITE

PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Coiled coil; Hydrolase; Membrane; Nucleotide-binding; Nucleotidyltransferase; Protease; Ribosomal frameshifting; RNA replication; RNA-directed RNA polymerase; Thiol protease; Transferase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1417`	`1417`	`Non-structural polyprotein 1AB.`	`PRO_0000327289`
`CHAIN`	`1 176`	`176`	`Protein p19 (Potential).`	`PRO_0000327290`
`CHAIN`	`177 420`	`244`	`Transmembrane protein 1A (Potential).`	`PRO_0000327291`
`CHAIN`	`421 665`	`245`	`Serine protease p27 (Potential).`	`PRO_0000327292`
`CHAIN`	`666 914`	`249`	`Protein p20 (Potential).`	`PRO_0000327293`
`CHAIN`	`915 1417`	`503`	`RNA-directed RNA polymerase p57 (Potential).`	`PRO_0000327294`
`TRANSMEM`	`155 175`	`21`	`Potential.`
`TRANSMEM`	`240 260`	`21`	`Potential.`
`TRANSMEM`	`287 307`	`21`	`Potential.`
`TRANSMEM`	`314 334`	`21`	`Potential.`
`TRANSMEM`	`345 365`	`21`	`Potential.`
`DOMAIN`	`1162 1288`	`127`	`RdRp catalytic.`
`COILED`	`105 143`	`39`	`Potential.`
`COILED`	`588 615`	`28`	`Potential.`
`ACT_SITE`	`462 462`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`490 490`		`Charge relay system; for serine protease activity (By similarity).`
`ACT_SITE`	`552 552`		`Charge relay system; for serine protease activity (By similarity).`
`SITE`	`176 177`	`2`	`Cleavage (Potential).`
`SITE`	`420 421`	`2`	`Cleavage (Potential).`
`SITE`	`665 666`	`2`	`Cleavage (Potential).`
`SITE`	`914 915`	`2`	`Cleavage (Potential).`

Sequence information

Length: 1417 AA [This is the length of the unprocessed precursor]

Molecular weight: 161559 Da [This is the MW of the unprocessed precursor]

CRC64: 94F6E43DBE61548B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMALGEPYYS SKPDKDFNFG STMARRQMTP TMVTKLPKFV RNSPQAYDWI VRGLIFPTTG 

        70         80         90        100        110        120 
KTYFQRVVVI TGGLEDGTYG SYAFNGSEWV EIYPIEHLNL MSSLKLIHKA NALQERLRLS 

       130        140        150        160        170        180 
QEEKATLALD VQFLQHENVR LKELIPKPEP RKIQMKWIIV GAVLTFLSLI PGGYAQSQTN 

       190        200        210        220        230        240 
NTIFTDMIAA CKYSTETLTE NLDLRIKLAL ANITINDKLD AVRQILNFAF VPRAHWLRTV 

       250        260        270        280        290        300 
FYYIHYYEMW NIFMFVLAIG TVMRSARPGT DLITLATSHL SGFRMAVLPT IPFHTTMTLW 

       310        320        330        340        350        360 
VMNTLMVCYY FDNLLAITMA ILAPILGIIF LCFMEDSNYV SQIRGLIATA VLIAGGHACL 

       370        380        390        400        410        420 
TLTGTTTSLF VVILTCRFIR MATVFIGTRF EIRDANGKVV ATVPTRIKNV AFDFFQKLKQ 

       430        440        450        460        470        480 
SGVRVGVNDF VVIKPGALCI IDTPEGKGTG FFSGNDIVTA AHVVGNNTFV SVCYEGLVYE 

       490        500        510        520        530        540 
AKVRYMPEKD IAFITCPGDL HPTARLKLSK NPDYSCVTVM AYVNEDLVVS TATAMVHGNT 

       550        560        570        580        590        600 
LSYAVRTQDG MSGAPVCDKY GRVLAVHQTN TGYTGGAVII DPADFHPVKA PSQVELLKEE 

       610        620        630        640        650        660 
IERLKAQLNS AAENPVTVVT QQPIVTLEQK SVSDSDVVDL VRTAMEREMK VLRDEINGIL 

       670        680        690        700        710        720 
APFLQKKKGK TKHGRGRVRR NLRKGVKLLT EEEYRELLEK GLDRETFLDL IDRIIGERSG 

       730        740        750        760        770        780 
YPDYDDEDYY DEDDDGWGMV GDDVEFDYTE VINFDQAKPT PAPRTTKPKP CPEPKIEAQP 

       790        800        810        820        830        840 
LDLSQKKEKQ PEHEQQVAKP TKPQKIEPQP YSQTYGKAPI WESYDFDWDE DDAKFILPAP 

       850        860        870        880        890        900 
HRLTKADEIV LGSKIVKLRT IIETAIKTQN YSALPEAVFE LDKAAYEAGL EGFLQRVKSK 

       910        920        930        940        950        960 
KQGPKKLQRA PEDQGAQNYH SLDAWKSLLE PPRERRCVPA NFPLLGHLPI NRPIFDDKKP 

       970        980        990       1000       1010       1020 
RDDLLGLLPE PTWHAFEEYG PTTWGPQAFV KSFDKFFYAE PIDFFSEYPQ LCAFADWATY 

      1030       1040       1050       1060       1070       1080 
REFRYLEDTR VIHITATEKN TDSTPAYPKM NYFDTEEDYL EAHGWAPYIR EFTRVFKGDK 

      1090       1100       1110       1120       1130       1140 
PEVLWYLFLK KEIIKEEKIR NSDIRQIVCA DPIYTRIGAC LEAHQNALMK QHTDTSVGQC 

      1150       1160       1170       1180       1190       1200 
GWSPMEGGFK KTMQRLVNKG NKHFIEFDWT RYDGTIPPAL FKHIKEIRWN FINKDQREKY 

      1210       1220       1230       1240       1250       1260 
RHVHEWYVDN LLNRHVLLPS GEVTLQTRGN PSGQFSTPMD NNMVNFWLQA FEFAYFNGPD 

      1270       1280       1290       1300       1310       1320 
KDLWKTYDTV VYGDDRLSTT PSVPDNYEER VITMYRDIFG MWVKPGKVIC RDSIVGLSFC 

      1330       1340       1350       1360       1370       1380 
GFTVNENLEP VPTSPEKLMA SLLKPYKILP DLESLHGKLL CYQLLAAFMA EDHPFKVYVE 

      1390       1400       1410 
HCLSRTAKQL RDSGLPARLT EEQLHRIWRG GPKKCDG

Q9IFX2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools