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UniProtKB/Swiss-Prot entry Q9HMX8

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DP2L_HALSA
Primary accession number Q9HMX8
Secondary accession numbers None
Integrated into Swiss-Prot on April 27, 2001
Sequence was last modified on March 1, 2001 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 52)
Name and origin of the protein
Protein name DNA polymerase II large subunit
Synonyms Pol II
EC 2.7.7.7
Contains Hsp-NRC1 polC intein
     (Hsp-NRC1 pol2 intein)
Gene name
Name: polC
Synonyms: polA2
OrderedLocusNames: VNG_2338G
From
Halobacterium salinarium (Halobacterium halobium) [TaxID: 2242] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Halobacteria; Halobacteriales; Halobacteriaceae; Halobacterium.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
DOI=10.1073/pnas.190337797; PubMed=11016950 [NCBI, ExPASy, EBI, Israel, Japan]
Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S.;
"Genome sequence of Halobacterium species NRC-1.";
Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
Comments
  • FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
  • CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
  • CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
  • SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
  • PTM: This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation (Potential).
  • SIMILARITY: Belongs to the archaeal DNA polymerase II family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE004437; AAG20443.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR G84384; G84384.
RefSeq NP_280963.1; -.
3D structure databases
ModBase Q9HMX8.
Enzyme and pathway databases
BioCyc HSP64091:VNG2338G-MON; -.
Ontologies
GO
GO:0008408; Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887; Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738; Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261; Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00324; -; 1.
PBIL [Tree]
InterPro IPR003586; Hedgehog_hint_C.
IPR003587; Hedgehog_hint_N.
IPR006141; Intein_splicing_site.
IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.
Pfam PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF016275; PolC_DP2; 1.
SMART SM00305; HintC; 1.
SM00306; HintN; 1.
SMART graphical view of domain structure.
TIGRFAMs TIGR01443; intein_Cterm; 1.
TIGR01445; intein_Nterm; 1.
TIGR00354; polC; 1.
PROSITE PS50818; INTEIN_C_TER; FALSE_NEG.
PS50817; INTEIN_N_TER; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9HMX8.
Genome annotation databases
GeneID 1448745; -.
GenomeReviews AE004437_GR; VNG_2338G.
KEGG hal:VNG2338G; -.
NMPDR fig|64091.1.peg.1990; -.
Phylogenomic databases
HOGENOM Q9HMX8; -.
Genome annotation databases
CMR Q9HMX8; VNG_2338G.
Other
ProtoNet Q9HMX8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Autocatalytic cleavage; Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Protein splicing; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1    925  925     DNA polymerase II large subunit, 1st part (Potential). PRO_0000007301
CHAIN   926   1120  195     Hsp-NRC1 polC intein (Potential). PRO_0000007302
CHAIN   1121   1370  250     DNA polymerase II large subunit, 2nd part (Potential). PRO_0000007303
Sequence information
Length: 1370 AA [This is the length of the unprocessed precursor] Molecular weight: 150296 Da [This is the MW of the unprocessed precursor] CRC64: 07878AA9976790C9 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MRPTDETYFE TLETGLDDAF EVAEAARERG EDPTPNVEIP VAKDMADRVE NILGIDGVAE 

        70         80         90        100        110        120 
RVRDLDGEMS REEAALELVA DFVDGRVGDY DTDAGKIEGA VRTAVALLTE GVVAAPIEGI 

       130        140        150        160        170        180 
DRVEVNDNDD GTQYVAVYYA GPIRSAGGTA QALSVLVADY ARAMLGIDAF KPRDDEIERY 

       190        200        210        220        230        240 
AEEVDLYDSE TGLQYSPKDA ETTFITEHCP VMLDGEATGN EEVDGFRDLE RIDTNSPRGG 

       250        260        270        280        290        300 
MCLVLAEGIA LKAPKIQRYT RNLDEVAWPW LQDLIDGTIG ADDADEDTPD AGSDSDATDE 

       310        320        330        340        350        360 
GDAPSASTDA EEPPRAAPSD KFLRDLIAGR PVFGHPSKNG GFRLRYGRAR NHGNATAGVH 

       370        380        390        400        410        420 
PATMHLLDDF LATGTQIKTE RPGKAAGIVP VDSIEGPTVK LANGDVRHIN DPADALDVRN 

       430        440        450        460        470        480 
GVAEILDVGE YLVNYGEFVE NNHELAPASY APEWWIQDLD AAGADVQALR DSPYVDLTAP 

       490        500        510        520        530        540 
TADQAMAWAT DYDAPLHPAY TYLWHDIDVE QFRALADAVA DAHTDASDDD DRGVLVLDHT 

       550        560        570        580        590        600 
TTVRETLEAL LVTHHQGDDT IRVDDWLPLA RSLGVTESLD REWETLSEAA AEWPNAVRAV 

       610        620        630        640        650        660 
NEVAPFSVQE RAPTRIGNRM GRPEKSESRD LSPAVHTLFP IGDAGGSQRD VADAARYAPD 

       670        680        690        700        710        720 
MSDTPGEIPV RVGDRVCPSC DEHTYESRCP DCGDWTDPHY ECRDCGAVAT PDESGRVECP 

       730        740        750        760        770        780 
NCGRDLDNVT TQVIDINDEY HGALRAVGER ENAFDQLKGV KGLLSAEKTP EPMAKGVLRA 

       790        800        810        820        830        840 
KHDVTAFKDG TVRYDMTDLP VTAVTPAELD VTAGQFRELG YNQDIHGDPL EHDDQLVELR 

       850        860        870        880        890        900 
VQDVVLSDGA ADHMLKTADF VDDLLTQYYG LDAFYDLDDR DDLVGELVFG MAPHTSAAVV 

       910        920        930        940        950        960 
GRVAGFTSAS VGYAHPYFHA AKRRNCFHPE TNVWFRDESG EWHHDPIETL VEARLDPDTA 

       970        980        990       1000       1010       1020 
DEDDFGALVQ ALDGDVFVPS VTEDGEETLQ RVEAVSKHPA PDHLLAVETK RGRELTVTPD 

      1030       1040       1050       1060       1070       1080 
HSMRRWTGDG IERVDARELT AGDALPAPTQ VPGDGETATS ELRSESLDGT HPQRRFGDGG 

      1090       1100       1110       1120       1130       1140 
SVRTDEVVSV EPVRSSVDHT YSLTVAETNT LVANGLFTGQ CDGDEDCVML LMDGLINFSK 

      1150       1160       1170       1180       1190       1200 
SYLPDKRGGR MDAPLVMSSR IDPAEIDDEA HNIDIDREYP REFYEATREL ADPEDVADLI 

      1210       1220       1230       1240       1250       1260 
TLAESTVGTD EEYTGFGHTH ATSNIHLGPS LSAYKTLGSM MDKMDAQLEL ARKLRSVAET 

      1270       1280       1290       1300       1310       1320 
DVAERVIEYH FLPDLIGNLR AFSRQETRCL DCGEKYRRMP LSGDCRECGG RVNLTVHEGS 

      1330       1340       1350       1360       1370 
VNKYMDTAMR VATEYDCREY TKQRLEIMDR RLESVFEDDT NKQSGISDFM
Q9HMX8 in FASTA format

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