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UniProtKB/Swiss-Prot entry Q9H492


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MLP3A_HUMAN
Primary accession number Q9H492
Secondary accession number Q9BXW5
Integrated into Swiss-Prot on November 8, 2002
Sequence was last modified on May 10, 2005 (Sequence version 2)
Annotations were last modified on    September 2, 2008 (Entry version 50)
Name and origin of the protein
Protein name Microtubule-associated proteins 1A/1B light chain 3A [Precursor]
Synonyms Microtubule-associated protein 1 light chain 3 alpha
MAP1A/1B light chain 3 A
MAP1A/MAP1B LC3 A
MAP1 light chain 3-like protein 1
Autophagy-related protein LC3 A
Autophagy-related ubiquitin-like modifier LC3 A
Gene name
Name: MAP1LC3A
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MUTAGENESIS OF GLY-120.
DOI=10.1074/jbc.M303800200; PubMed=12740394 [NCBI, ExPASy, EBI, Israel, Japan]
He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J.O., Yu L.;
"Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B.";
J. Biol. Chem. 278:29278-29287(2003).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
TISSUE=Amygdala;
The German cDNA consortium;
Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (ISOFORMS 1 AND 2).
DOI=10.1038/414865a; PubMed=11780052 [NCBI, ExPASy, EBI, Israel, Japan]
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
"The DNA sequence and comparative analysis of human chromosome 20.";
Nature 414:865-871(2001).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
TISSUE=Uterus;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
LIPIDATION, AND CLEAVAGE BY APG4B.
DOI=10.1074/jbc.M401461200; PubMed=15187094 [NCBI, ExPASy, EBI, Israel, Japan]
Tanida I., Sou Y.-S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E.;
"HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates.";
J. Biol. Chem. 279:36268-36276(2004).
Comments
  • FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity).
  • SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes.
  • ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
    Name1
    Isoform IDQ9H492-1
    This is the isoform sequence displayed in this entry.
    Name2
    Isoform IDQ9H492-2
    Note: No experimental confirmation available.
    Features which should be applied to build the isoform sequence: VSP_013660.
  • TISSUE SPECIFICITY: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • PTM: The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • SIMILARITY: Belongs to the MAP1 LC3 family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF276658; AAK35151.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL833855; CAD38714.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT007452; AAP36120.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL118520; CAC14078.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL118520; CAI40290.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC015810; AAH15810.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_115903.1; -.
NP_852610.1; -.
UniGene Hs.632273
3D structure databases
HSSP O08765; 1EO6. [HSSP ENTRY / PDB]
SMR Q9H492; 1-120.
ModBase Q9H492.
Organism-specific databases
H-InvDB HIX0015748; -.
HGNC HGNC:6838; MAP1LC3A.
GenAtlas MAP1LC3A.
HPA HPA007649; -.
MIM 601242; gene. [NCBI / EBI]
PharmGKB PA30582; -.
GeneCards Q9H492.
Gene expression databases
CleanEx HS_MAP1LC3A; -.
GermOnline ENSG00000101460; Homo sapiens.
Ontologies
GO
GO:0005776; Cellular component: autophagic vacuole (inferred from direct assay from UniProtKB).
GO:0005829; Cellular component: cytosol (inferred from direct assay from UniProtKB).
GO:0031090; Cellular component: organelle membrane (inferred from direct assay from UniProtKB).
GO:0008429; Molecular function: phosphatidylethanolamine binding (inferred from direct assay from UniProtKB).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from UniProtKB).
GO:0000045; Biological process: autophagosome formation (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR004241; MAP1_LC3.
Graphical view of domain structure.
PANTHER PTHR10969; MAP1_LC3; 1.
Pfam PF02991; MAP1_LC3; 1.
Pfam graphical view of domain structure.
BLOCKS Q9H492.
Genome annotation databases
Ensembl ENSG00000101460; Homo sapiens. [Contig view]
GeneID 84557; -.
KEGG hsa:84557; -.
Phylogenomic databases
HOVERGEN Q9H492; -.
Other
SOURCE MAP1LC3A; Homo sapiens.
ProtoNet Q9H492.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Autophagy; Cytoplasm; Cytoplasmic vesicle; Lipoprotein; Membrane; Microtubule; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   120  120     Microtubule-associated proteins 1A/1B light chain 3A. PRO_0000017192
PROPEP   121   121  1     Removed in mature form. PRO_0000017193
LIPID   120   120        Phosphatidylethanolamine amidated glycine (Probable). 
VAR_SEQ   1    13        MPSDRPFKQRRSF -> MKMRFFSSPCGKAAVDP (in isoform 2). VSP_013660
MUTAGEN   120   120        G->A: No processing of precursor. 
Sequence information
Length: 121 AA [This is the length of the unprocessed precursor] Molecular weight: 14272 Da [This is the MW of the unprocessed precursor] CRC64: 48C1FBE8F7892AF3 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPSDRPFKQR RSFADRCKEV QQIRDQHPSK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 

        70         80         90        100        110        120 
SELVKIIRRR LQLNPTQAFF LLVNQHSMVS VSTPIADIYE QEKDEDGFLY MVYASQETFG 


F 

Q9H492 in FASTA format

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