NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT MET-63.
DOI=10.1007/s003350010253; PubMed=11252170 [NCBI, ExPASy, EBI, Israel, Japan]
Haworth K.E., Putt W., Cattanach B., Breen M., Binns M., Lingaas F., Edwards Y.H.;
"Canine homolog of the T-box transcription factor T; failure of the protein to bind to its DNA target leads to a short-tail phenotype.";
Mamm. Genome 12:212-218(2001).

Comments

FUNCTION: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site (By similarity).
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Nucleus.
POLYMORPHISM: A mutation in position 63 is responsible for a short-tail trait in a bob-tailed dog. The homozygous mutation is lethal.
SIMILARITY: Contains 1 T-box DNA-binding domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ245513; CAC10530.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001003092.1; -.

UniGene

Cfa.3518

3D structure databases

HSSP

P24781; 1XBR. [HSSP ENTRY / PDB]

SMR

Q9GL27; 41-224.

ModBase

Q9GL27.

Family and domain databases

InterPro

IPR002070; TF_Brachyury.
IPR001699; TF_T-box.
Graphical view of domain structure.

Gene3D

G3DSA:2.60.40.820; TF_T-box; 1.

PANTHER

PTHR11267; TF_T-box; 1.

Pfam

PF00907; T-box; 1.
Pfam graphical view of domain structure.

PRINTS

PR00938; BRACHYURY.
PR00937; TBOX.

SMART

SM00425; TBOX; 1.
SMART graphical view of domain structure.

PROSITE

PS01283; TBOX_1; 1.
PS01264; TBOX_2; 1.
PS50252; TBOX_3; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9GL27.

Genome annotation databases

Ensembl

ENSCAFG00000000807; Canis familiaris. [Contig view]

GeneID

403653; -.

KEGG

cfa:403653; -.

Phylogenomic databases

HOVERGEN

Q9GL27; -.

Other

ProtoNet

Q9GL27.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Activator; Developmental protein; DNA-binding; Nucleus; Polymorphism; Transcription; Transcription regulation.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 435`	`435`	`Brachyury protein.`	`PRO_0000184413`
`DNA_BIND`	`51 219`	`169`	`T-box.`
`VARIANT`	`63 63`	`1`	`I -> M (in a bob-tailed dog; alters the ability of the protein to bind to its consensus DNA target).`

Sequence information

Length: 435 AA [This is the length of the unprocessed precursor]

Molecular weight: 47148 Da [This is the MW of the unprocessed precursor]

CRC64: CDCDB4A53A395AE2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSPGAESAG KSLQYRVDHL LSAVESELQA GSEKGDPTER ELRVGLEDSE LWLRFKELTN 

        70         80         90        100        110        120 
EMIVTKNGRR MFPVLKVNVS GLDPNAMYSF LLDFVAADNH RWKYVNGEWV PGGKPEPQAP 

       130        140        150        160        170        180 
SCVYIHPDSP NFGAHWMKAP VSFSKVKLTN KLNGGGQIML NSLHKYEPRI HIVRVGGAQR 

       190        200        210        220        230        240 
MITSHCFPET QFIAVTAYQN EEITALKIKY NPFAKAFLDA KERSDHKDMM EEPGDSQQSG 

       250        260        270        280        290        300 
YSQWGWLIPG TSTLCPPATP HPQFGGPLSL PSTHGCERYP ALRNHRPSPY PSPYAHRNNS 

       310        320        330        340        350        360 
PTYSDNPSAC LSMLQSHDSW PSLGTPAHTS MLPMSHSAGP PTGSSQYPSL WSVSNGTITP 

       370        380        390        400        410        420 
GAQPAGMSNG LGAQFFRGSS AHGAPLGHAV PAPSASGSPL YEGAPTATDV PDSQYDASAQ 

       430 
ARLIASWTPV SPPSM

Q9GL27 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools