[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. STRAIN=129/Sv, and C57BL/6; DOI=10.1007/s003350010089; PubMed=10818213 [NCBI, ExPASy, EBI, Israel, Japan] Wang C.-C., Brodnicki T., Copeland N.G., Jenkins N.A., Harvey R.P.; "Conserved linkage of NK-2 homeobox gene pairs Nkx2-2/2-4 and Nkx2-1/2-9 in mammals."; Mamm. Genome 11:466-468(2000).
[2]	PARTIAL NUCLEOTIDE SEQUENCE. STRAIN=C57BL/6; DOI=10.1016/0896-6273(92)90291-K; PubMed=1346742 [NCBI, ExPASy, EBI, Israel, Japan] Price M., Lazzaro D., Pohl T., Mattei M.-G., Ruether U., Olivo J.-C., Duboule D., di Lauro R.; "Regional expression of the homeobox gene Nkx-2.2 in the developing mammalian forebrain."; Neuron 8:241-255(1992).

Comments

FUNCTION: Probable transcription factor.
SUBCELLULAR LOCATION: Nucleus (By similarity).
TISSUE SPECIFICITY: In the embryo it is detected in the posterior hypotalamus and later in the head. In the adult it is detected only in testis.
DEVELOPMENTAL STAGE: Expressed in a restricted region of the posterior hypothalamus from E10. Detected in the head region from E12.5 to E14.5. Expression is down-regulated by E15.5.
SIMILARITY: Belongs to the NK-2 homeobox family.
SIMILARITY: Contains 1 homeobox DNA-binding domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF202038; AAG35618.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF202039; AAG35619.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00111298; -.

NP_075993.1; -.

3D structure databases

HSSP

P23441; 1FTT. [HSSP ENTRY / PDB]

SMR

Q9EQM3; 188-254.

ModBase

Q9EQM3.

Organism-specific databases

MGI

MGI:97349; Nkx2-4.

Gene expression databases

ArrayExpress

Q9EQM3; -.

Bgee

Q9EQM3; -.

GermOnline

ENSMUSG00000054160; Mus musculus.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from electronic annotation from UniProtKB-SubCell).
GO:0043565;	Molecular function: sequence-specific DNA binding (inferred from electronic annotation from InterPro).
GO:0003700;	Molecular function: transcription factor activity (inferred from electronic annotation from InterPro).
GO:0007275;	Biological process: multicellular organismal development (inferred from electronic annotation from UniProtKB-KW).
GO:0006355;	Biological process: regulation of transcription, DNA-dependent (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR001356; Homeobox.
IPR017970; Homeobox_CS.
IPR012287; Homeodomain-rel.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.10.60; Homeodomain-rel; 1.

Pfam

PF00046; Homeobox; 1.
Pfam graphical view of domain structure.

ProDom

PD000010; Homeobox; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00389; HOX; 1.
SMART graphical view of domain structure.

PROSITE

PS00027; HOMEOBOX_1; 1.
PS50071; HOMEOBOX_2; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSMUSG00000054160; Mus musculus. [Contig view]

GeneID

228731; -.

KEGG

mmu:228731; -.

Phylogenomic databases

HOGENOM

Q9EQM3; -.

HOVERGEN

Q9EQM3; -.

OMA

Q9EQM3; PPGVSQF.

Other

NextBio

379096; -.

SOURCE

Nkx2-4; Mus musculus.

ProtoNet

Q9EQM3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Developmental protein; DNA-binding; Homeobox; Nucleus.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 354`	`354`	`Homeobox protein Nkx-2.4.`	`PRO_0000048936`
`DNA_BIND`	`188 247`	`60`	`Homeobox.`
`COMPBIAS`	`76 91`	`16`	`Poly-Ala.`
`COMPBIAS`	`132 136`	`5`	`Poly-Ala.`
`COMPBIAS`	`263 272`	`10`	`Poly-Pro.`

Sequence information

Length: 354 AA [This is the length of the unprocessed precursor]

Molecular weight: 36225 Da [This is the MW of the unprocessed precursor]

CRC64: A7A0F450DB15616C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSLSPKHTTP FSVSDILSPI EETYKKFGGV MDGAPPGLGA PLGAAAYRAP PSGPSSQAAA 

        70         80         90        100        110        120 
VAAGMQPPHA MAGHNAAAAA AAAAAAAAAA ATYHMPPGVS QFPHSAMGSY CNGGLGNMGE 

       130        140        150        160        170        180 
LPAYTDGMRG GAAAAATGWY GANTDPRYSS ISRFMGPSAG VNVAGMGSLT GIADAAKSLA 

       190        200        210        220        230        240 
PLHAAAPRRK RRVLFSQAQV YELERRFKQQ KYLSAPEREH LASMIHLTPT QVKIWFQNHR 

       250        260        270        280        290        300 
YKMKRQAKDK AAQQLQQEGG LGPPPPPPPP SPRRVAVPVL VKDGKPCQNG AGTPTPGQGG 

       310        320        330        340        350 
QQPQAPTPAP ELEELSPSPP ALHGPGGGLA ALDAATGDYG GGVLGANLLY GRTW

Q9EQM3 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools