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UniProtKB/Swiss-Prot entry Q9E784

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_BEFV
Primary accession number Q9E784
Secondary accession numbers None
Integrated into Swiss-Prot on August 21, 2007
Sequence was last modified on March 1, 2001 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 28)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Bovine ephemeral fever virus (BEFV) [TaxID: 11303] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Dimarhabdovirus supergroup; Ephemerovirus.
Virus hosts Bos taurus (Bovine) [TaxID: 9913]
Bubalus bubalis (Domestic water buffalo) [TaxID: 89462]
Culicoides [TaxID: 58271]
Syncerus caffer (Cape buffalo) [TaxID: 9970]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
STRAIN=BB7721;
DOI=10.1016/S0168-1702(00)00215-X; PubMed=11074128 [NCBI, ExPASy, EBI, Israel, Japan]
Dhillon J., Cowley J.A., Wang Y., Walker P.J.;
"RNA polymerase (L) gene and genome terminal sequences of ephemeroviruses bovine ephemeral fever virus and Adelaide River virus indicate a close relationship to vesiculoviruses.";
Virus Res. 70:87-95(2000).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Interacts with the P protein (By similarity).
  • SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the rhabdoviridae protein L family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF234533; AAG10420.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_065409.1; -.
3D structure databases
ModBase Q9E784.
Ontologies
GO
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
QuickGo view.
Family and domain databases
InterPro IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF037546; RNA_pol_RhabdoV_sub; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9E784.
Genome annotation databases
GeneID 911734; -.
Other
ProtoNet Q9E784.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   2144  2144     Large structural protein. PRO_0000297833
DOMAIN   624    810  187     RdRp catalytic. 
Sequence information
Length: 2144 AA [This is the length of the unprocessed precursor] Molecular weight: 249787 Da [This is the MW of the unprocessed precursor] CRC64: 4C6C3E6701D340BC [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDSVDFLDSE DHDQNSWNGE EFGDDFLEYI WDQDEEESMD LINNKDYNLN SPLIIDPLVE 

        70         80         90        100        110        120 
LRNWINNEKG HSESDLRSQQ SFEFKEFDII KRVLRNFINN VNLRRPEDSH HIFAKFLNQA 

       130        140        150        160        170        180 
IVTNDYWNLG ETLSKIVEDI REVGASFYRG LDLDENIIID NIRRNWNDVP QLGKSWFLKF 

       190        200        210        220        230        240 
FELHRVICVM NARSNIELKN LQSKNKLKKI KLPKELEERG HKCWIFDLDI SGRWIIFDNY 

       250        260        270        280        290        300 
AYWELQRIVL NREFILMMKD VLISRFQTVL SMNVCTDEYK YTEENIETMM SLYREGDLIL 

       310        320        330        340        350        360 
EEHGNKSYKG LKLLESICNL RLIKIVRKSR PKIPEFPNFE NHIYSSLNDL RVERGIDLSK 

       370        380        390        400        410        420 
FSNIILREES IDMVLAFYSS FRHFGHPWID YLTGLDKLES QVNKDCQVDI QYANLLASDL 

       430        440        450        460        470        480 
AFKILRKNFL EKKCWSVDKN KMDKKHKLYH HISHNTWPTQ QIIDEFGDHW HELPIIQCYE 

       490        500        510        520        530        540 
IPDMIDISQI YSDKSHSLDR SEVLKIIKEQ KHKRIPTKRV LQTLLEKPAT NWPEFLKAVN 

       550        560        570        580        590        600 
DYGLDWEKLV IGLKAKEREL KEEGRFFSLM SYELRDYFVS TEYLIKKYFV PLFEGLTMAD 

       610        620        630        640        650        660 
DLNTVIKKML DVSSGQGTRE YEYITIANNI DYEKWNNYQR IESNGPVFTV MGRFLGLPNL 

       670        680        690        700        710        720 
FTRTHEFFQK SLIYYNQRPD LMMVRGRECL NRLGVKVCWE GQKGGLEGLR QKGWSILNYL 

       730        740        750        760        770        780 
MIERESRVRN TRVKILAQGD NQTISMCYKT ESWQNEEELD NHIKNMVSNN NQIMQAIING 

       790        800        810        820        830        840 
TEKLGLRINL DETMTSADYI NYGKVPIIEG TIKGLPTKRW SRVNFTSNDQ LPSTSTVINS 

       850        860        870        880        890        900 
SSTNALTVGH FSERPHDAIN GHLLFGSLGL LLLDYHNPAI RGQISEFIPE ANINNKLYNI 

       910        920        930        940        950        960 
LLLYLDPSLG GIAGTSLTRF FIRGFPDGVT ESLTFWKIVG EYTNDQDIKR LASTVGSPEL 

       970        980        990       1000       1010       1020 
SPFKPEDLDK LIEKPESLNI KHGLSSSNMI KGEVKKNIIE NCSKIQNEII RDAARNLVSE 

      1030       1040       1050       1060       1070       1080 
ENQLFLWLRT INPLFPRFLS QFAESTYYGV TKSLINLFTN SKTIRGIYKK KYRKELDQLM 

      1090       1100       1110       1120       1130       1140 
IKGEVRSIFG LIKIVNRSKQ FVMPIWDCSA SLADSLRKRS WGKEVLGTTV PHPAEMFKGY 

      1150       1160       1170       1180       1190       1200 
RGGEDSCSFC RGNGSNNNYL TVLMPRGIPM KCHYRGPYYP YLGSNTKEST SILQPWEKET 

      1210       1220       1230       1240       1250       1260 
KVPVLKRACD LRKSINWFVT PDSLLAKSIF NNLKALTGED WEDQIKGYKR TGSSLHRFGC 

      1270       1280       1290       1300       1310       1320 
SRVSSGGFSA SSPSCFTWCI ATTDTMCGLG EVNYDFMFQS TLVWCQMSSI IRERGNLHSK 

      1330       1340       1350       1360       1370       1380 
IHHYHIKCNK CLREIQEPVL ESGWEYQPRN VSQILEKWRP KNMKTWGEEK IHMDIKDNDD 

      1390       1400       1410       1420       1430       1440 
EWDNLTVEDK SYEIGKTIGW LVGDSLLSHK RNYEFKSLFP VSIRYKLEGL PFLEGILDGF 

      1450       1460       1470       1480       1490       1500 
KICGSLNLTH RRNYMILKKP KLALQGTVFF LIDRSSFISE FTNFISHPKI YKAIKSLPHK 

      1510       1520       1530       1540       1550       1560 
IPTSYPMNLS DLGSILRSFL KQLYYRRKEL IIKKSSWVFS DMRTNEVICS FGLSHLTYRI 

      1570       1580       1590       1600       1610       1620 
LIQEGLNKDM KLRLQQCQDL YINIMTESKE ELDKSSAKRE IVECLRELKF VSSEIRHAVK 

      1630       1640       1650       1660       1670       1680 
FRYITGEKGE VQLIKEMEER RTWGDEYTGK ANLLDVWYLT SQSSENKNLV KGIKIPYHSN 

      1690       1700       1710       1720       1730       1740 
PTISGLRINQ IATGAHYKLR TLINMTRITY RDFICGGDGS GGMTSCLLRL KPLSRGVFNS 

      1750       1760       1770       1780       1790       1800 
LLILDDKPLH GTRPSPPTAI MELGEDSLRC VNCYDVWKEP SDLSKQETWK YFVKLKKQNS 

      1810       1820       1830       1840       1850       1860 
MMIDLIVLDM EIINDEVIED IYQNTKNHLI YLLEEGGCLI IKTYLTYLLK ENTNILDMLG 

      1870       1880       1890       1900       1910       1920 
HLFTSVQLIN TNLSSMKTSE IYVLFKNYKN RLTPCLQFDR NIILDNWSFF YINKPIREEF 

      1930       1940       1950       1960       1970       1980 
ERARELLNED LGMGMPKELE PNPLIELSQM LQNSGVDGVA MSAISQEENL SFFSTKELAV 

      1990       2000       2010       2020       2030       2040 
ICLIIISESH LVTTKKHKND CNCLQKKPYS DQEIKSWMSG IIGIGLYLSL LDNKTNSFEI 

      2050       2060       2070       2080       2090       2100 
LDYLINSDFK SIHVNFNKER ELCWSINYSS NEKKGKDLWK KRFSVKDKMA FMGNWIRLLH 

      2110       2120       2130       2140 
RQKVKNQKCE YKEGRINNFL KFINKGLNLQ KVKSQYEEEI AKLL
Q9E784 in FASTA format

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