RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Sendai virus (strain Hamamatsu) (SeV)

[TaxID: 302271]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Respirovirus.

Virus hosts

Cavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]
Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. DOI=10.1023/A:1008130318633; PubMed=11210938 [NCBI, ExPASy, EBI, Israel, Japan] Fujii Y., Kiyotani K., Yoshida T., Sakaguchi T.; "Conserved and non-conserved regions in the Sendai virus genome: evolution of a gene possessing overlapping reading frames."; Virus Genes 22:47-52(2001).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate E15cl2; DOI=10.1128/JVI.76.17.8540-8547.2002; PubMed=12163573 [NCBI, ExPASy, EBI, Israel, Japan] Fujii Y., Sakaguchi T., Kiyotani K., Huang C., Fukuhara N., Egi Y., Yoshida T.; "Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA."; J. Virol. 76:8540-8547(2002).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate E15cl2, Isolate E30cl2, and Isolate E30M15cl5; DOI=10.1023/A:1020165919672; PubMed=12416681 [NCBI, ExPASy, EBI, Israel, Japan] Fujii Y., Sakaguchi T., Kiyotani K., Huang C., Fukuhara N., Yoshida T.; "Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage."; Virus Genes 25:189-193(2002).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate E50cl9; Fujii Y., Kiyotani K., Huang C., Fukuhara N., Egi K., Yoshida T., Sakaguchi T.; Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently six subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals. 5' GpppApGpG sequence is required for mRNA cap methylation by the enzyme (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Homooligomer. Interacts with the P and C proteins. The L protein complexes with P protein to form the functional polymerase. C protein binding to L has an inhibitory effect (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
DOMAIN: The N-terminal part (about 1-400) seems to be involved in binding to the P protein (By similarity).
MISCELLANEOUS: Least abundant structural protein (approximately 50 copies per virion). Unstable in the absence of P protein (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB039658; BAB20026.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB065186; BAC79132.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB065187; BAC07512.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB065188; BAC79140.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB065189; BAC79148.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q9DUD8.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2228`	`2228`	`Large structural protein.`	`PRO_0000142736`
`DOMAIN`	`656 840`	`185`	`RdRp catalytic.`
`NP_BIND`	`1801 1810`	`10`	`ATP (Potential).`
`REGION`	`1 174`	`174`	`Oligomerization domain (By similarity).`
`REGION`	`1756 2228`	`473`	`Involved in mRNA cap methylation (By similarity).`
`COMPBIAS`	`2031 2034`	`4`	`Poly-Ser.`
`VARIANT`	`152 152`	`1`	`Q -> K (in strain: Isolate E50cl9; egg passage attenuated).`
`VARIANT`	`623 623`	`1`	`G -> S (in strain: Isolate E50cl9; egg passage attenuated).`
`VARIANT`	`626 626`	`1`	`K -> N (in strain: Isolate E30M15cl5; revertant of egg passage attenuated).`
`VARIANT`	`1207 1207`	`1`	`S -> C (in strain: Isolate E15cl2, Isolate E50cl9 and Isolate E30M15cl5; egg passage attenuated).`
`VARIANT`	`1791 1791`	`1`	`S -> N (in strain: Isolate E30cl2 and Isolate E50cl9; egg passage attenuated clones).`
`VARIANT`	`2024 2024`	`1`	`T -> I (in strain: Isolate E30M15cl5; revertant of egg passage attenuated).`

Sequence information

Length: 2228 AA [This is the length of the unprocessed precursor]

Molecular weight: 252984 Da [This is the MW of the unprocessed precursor]

CRC64: FCA1E42A19EBB759 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDGQESSQNP SDILYPECHL NSPIVRGKIA QLHVLLDVNQ PYILKDDSII NITKHKIRNG 

        70         80         90        100        110        120 
GLSPRQIKIR SLGKALQRTI KDLDRYTFEP YPTYSQELLR LDIPEICDKI RSVFAVSDRL 

       130        140        150        160        170        180 
TKELSNGFQD LWLNIFKQLG NIEGREGYDP LQDISTIPEI TERYSRNKWY RPFLTWFSIK 

       190        200        210        220        230        240 
YDMRWMQKTR PGGPLDTSNS HNLLECKSYT LVTYGDLVMI LNKLTLTGYI LTPELVLMYC 

       250        260        270        280        290        300 
DVVEGRWNMS AAGQLDKRST GITSKGEELW ELVDSLFSSL GEEIYNVIAL LEPLSLALIQ 

       310        320        330        340        350        360 
LSDPVIPLRG AFMRHVLTEL QTVLTSKDVY TDPEADAIVE SLLAIFHGTS IDEKAEIFSF 

       370        380        390        400        410        420 
FRTFGHPSLE AVTAADKVRA HMYAQKAIKL KTLHECHAVF CTIIINGYRE RHGGQWPPCD 

       430        440        450        460        470        480 
FPDHVCLELR NAQGSNTAIS YECAVDNYTS FIGFKFRKFI EPQLDEDLTI YMKDKALSPR 

       490        500        510        520        530        540 
KEAWDSVYPD SNLYYKVPES EETRRLIEVF INDENFNPED IIDYVESGDW LKDEKFNISY 

       550        560        570        580        590        600 
SLKEKEIKQE GRLFAKMTYK MRAVQVLAET LLAKGIGELF SENGMVKGEI DLLKRLTTLS 

       610        620        630        640        650        660 
VSGVPRTDSV YNNPRSSEKR NEGMKKRNSK GYWDEKKRSR HEFKATDSST DGYETLSCFL 

       670        680        690        700        710        720 
TTDLKKYCLN WRFESTALFG QRCNEIFGFK TFFNWMHPVL EKCTIYVGDP YCPVADRMHR 

       730        740        750        760        770        780 
QLQDHADSGI FIHNPRGGIE GYCQKLWTLI SISAIHLAAV RVGVRVSAMV QGDNQAIAVT 

       790        800        810        820        830        840 
SRVPVAQTYK QKKNHVYEEI TRYFGALRHV MFDIGHELKL NETIISSKMF VYSKRIYYDG 

       850        860        870        880        890        900 
KILPQCLKAL TRCVFWSETL VDENRSACSN ISTSIAKAIE NGYSPILGYC IALYKTCQQV 

       910        920        930        940        950        960 
CISLGMTINP TISPTVRDQY FKGKNWLRCA VLIPANVGGF NYMSTSRCFV RNIGDPAVAA 

       970        980        990       1000       1010       1020 
LADLKRFIRA DLLDKQVLYR VMNQEPGDSS FLDWASDPYS CNLPHSQSIT TIIKNITARS 

      1030       1040       1050       1060       1070       1080 
VLQESPNPLL SGLFTETSGE EDLNLASFLM DRKVILPRVA HEILGNSLTG VREAIAGMLD 

      1090       1100       1110       1120       1130       1140 
TTKSLVRASV KRGGLSYGIL RRLVNYDLLQ YETLTRTLRK PVKDNIEYEY MCSVELAVGL 

      1150       1160       1170       1180       1190       1200 
RQKMWIHLTY GRPIHGLETP DPLELLRGTF IEGSEVCKLC RSEGADPIYT WFYLPDNIDL 

      1210       1220       1230       1240       1250       1260 
DTLTNGSPAI RIPYFGSATD ERSEAQLGYV RNLSKPAKAA IRIAMVYTWA YGTDEISWME 

      1270       1280       1290       1300       1310       1320 
AALIAQTRAN LSLENLKLLT PVSTSTNLSH RLKDTATQMK FSSATLVRAS RFITISNDNM 

      1330       1340       1350       1360       1370       1380 
ALKEAGESKD TNLVYQQIML TGLSLFEFNM RYKKGSLEKP LILHLHLNNG CCIMESPQEA 

      1390       1400       1410       1420       1430       1440 
NIPPRSTLDL EITQENNKLI YDPDPLRDVD LELFSKVRDV VHTVDMTYWS DDEVIRATSI 

      1450       1460       1470       1480       1490       1500 
CTAMTIADTM SQLDRDNLKE MIALVNDDDV NSLITEFMVI DVPLFCSTFG GILVNQFAYS 

      1510       1520       1530       1540       1550       1560 
LYGLNIRGRE EIWGHVVRIL KDTSHAVLKV LSNALSHPKI FKRFWNAGVV EPVYGPNLSN 

      1570       1580       1590       1600       1610       1620 
QDKTLLALSV CEYSVDLFMH DWQGGVPLEV FICDNDPDVA DMRRSSFLAR HLAYLCSLAE 

      1630       1640       1650       1660       1670       1680 
ISRDGPRLES MNSLERLETL KSYLELTFLD DPVLRYSQLT GLVIKVFPST LTYIRKSSIK 

      1690       1700       1710       1720       1730       1740 
VLRTRGIGVP EVLEDWDPEA DNALLDGIAA EIQQNIPLGH QTRAPFWGLR VSKSQVLRLR 

      1750       1760       1770       1780       1790       1800 
GYEEITRGEV GRSGVGLTLP FDGRYLSHQL RLFGVNSTSC LKALELTYLL SPLVDKDKDR 

      1810       1820       1830       1840       1850       1860 
LFLGEGAGAM LSCYDATLGP CINYYNSGVY SCDVNGQREL NIYPAEVALV GKKLNNVTSL 

      1870       1880       1890       1900       1910       1920 
GQRVKVLFNG NPGSTWIGND ECEALIWNEL QNSSIGLVHC DMEGGDHKDD QVVLHEHYSV 

      1930       1940       1950       1960       1970       1980 
IRIAYLVGDR DVVLISKIAP RLGTDWTRQL SLYLRYWDEV NLVVLKTSNP ASTEMYLLSR 

      1990       2000       2010       2020       2030       2040 
HPKSDIIEDS KTVLASLHPL SKEDSIKIEK WILIEKAKAH EWVTRELREG SSSSGMLRPY 

      2050       2060       2070       2080       2090       2100 
HQALQTFGFE PNLYKLSRDF LSTMNIADTH NCMTAFNRVL KDTIFEWARI TESDKRLKLT 

      2110       2120       2130       2140       2150       2160 
GKYDLYPVRD SGKLKTISRR LVLSWVSLSM STRLVTGSFP DQKFEARLQL GIVSLSSREI 

      2170       2180       2190       2200       2210       2220 
RNLRVITKTI LDRFENTIHS ITYRFLTKEI KILMKILGAV KMFGARQNEY TTVVDDGSLD 


DIEPYDSL

Q9DUD8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools