RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Nipah virus

[TaxID: 121791]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Henipavirus.

Virus hosts

Cynopterus brachyotis (Lesser short-nosed fruit bat) [TaxID: 58060]
Eonycteris spelaea (Lesser dawn bat) [TaxID: 58065]
Homo sapiens (Human) [TaxID: 9606]
Pteropus hypomelanus (Island flying fox) (Variable flying fox) [TaxID: 9405]
Pteropus vampyrus (Large flying fox) [TaxID: 132908]
Scotophilus kuhlii [TaxID: 153297]
Sus scrofa (Pig) [TaxID: 9823]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. DOI=10.1126/science.288.5470.1432; PubMed=10827955 [NCBI, ExPASy, EBI, Israel, Japan] Chua K.B., Bellini W.J., Rota P.A., Harcourt B.H., Tamin A., Lam S.K., Ksiazek T.G., Rollin P.E., Zaki S.R., Shieh W., Goldsmith C.S., Gubler D.J., Roehrig J.T., Eaton B., Gould A.R., Olson J., Field H., Daniels P., Ling A.E., Peters C.J., Anderson L.J., Mahy B.W.; "Nipah virus: a recently emergent deadly paramyxovirus."; Science 288:1432-1435(2000).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. DOI=10.1006/viro.2001.1026; PubMed=11504554 [NCBI, ExPASy, EBI, Israel, Japan] Harcourt B.H., Tamin A., Halpin K., Ksiazek T.G., Rollin P.E., Bellini W.J., Rota P.A.; "Molecular characterization of the polymerase gene and genomic termini of Nipah virus."; Virology 287:192-201(2001).
[3]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate UMMC1, and Isolate UMMC2; PubMed=11514724 [NCBI, ExPASy, EBI, Israel, Japan] Chan Y.P., Chua K.B., Koh C.L., Lim M.E., Lam S.K.; "Complete nucleotide sequences of Nipah virus isolates from Malaysia."; J. Gen. Virol. 82:2151-2155(2001).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. STRAIN=Isolate NiV/MY/99/UM-0128, and Isolate NV/MY/99/VRI-1413,and Isolate NiV/MY/99/VRI-2794; PubMed=15663869 [NCBI, ExPASy, EBI, Israel, Japan] Abubakar S., Chang L.Y., Mohdali A.R., Sharifah S.H., Yusoff K., Zamrod Z.; "Isolation and molecular identification of Nipah virus from pigs."; Emerg. Infect. Dis. 10:2228-2230(2004).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF212302; AAK29089.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY029768; AAK50555.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ564622; CAD92358.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY029767; AAK50546.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ564621; CAD92352.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ564623; CAD92364.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ627196; CAF25498.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_112028.1; -.

3D structure databases

ModBase

Q997F0.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q997F0.

Genome annotation databases

GeneID

920950; -.

Other

ProtoNet

Q997F0.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2244`	`2244`	`Large structural protein.`	`PRO_0000236011`
`DOMAIN`	`715 899`	`185`	`RdRp catalytic.`
`NP_BIND`	`1840 1849`	`10`	`ATP (Potential).`
`VARIANT`	`223 223`	`1`	`T -> N (in strain: Isolate NiV/MY/99/VRI-0626).`
`VARIANT`	`1645 1645`	`1`	`S -> F (in strain: Isolate NiV/MY/99/UM-0128, Isolate NiV/MY/99/VRI-2794, Isolate UMMC1 and Isolate NiV/MY/99/VRI-0626).`
`VARIANT`	`1753 1753`	`1`	`M -> V (in strain: Isolate NiV/MY/99/VRI-0626).`
`VARIANT`	`2039 2039`	`1`	`H -> N (in strain: Isolate NiV/MY/99/VRI-0626).`

Sequence information

Length: 2244 AA [This is the length of the unprocessed precursor]

Molecular weight: 257233 Da [This is the MW of the unprocessed precursor]

CRC64: 1DE123E651A738C9 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MADELSISDI IYPECHLDSP IVSGKLISAI EYAQLRHNQP SDDKRLSENI RLNLHGKRKS 

        70         80         90        100        110        120 
LYILRQSKQG DYIRNNIKNL KEFMHIAYPE CNNILFSITS QGMTSKLDNI MKKSFKAYNI 

       130        140        150        160        170        180 
ISKKVIGMLQ NITRNLITQD RRDEIINIHE CRRLGDLGKN MSQSKWYECF LFWFTIKTEM 

       190        200        210        220        230        240 
RAVIKNSQKP KFRSDSCIIH MRDKSTEIIL NPNLICIFKS DKTGKKCYYL TPEMVLMYCD 

       250        260        270        280        290        300 
VLEGRMMMET TVKSDIKYQP LISRSNALWG LIDPLFPVMG NRIYNIVSMI EPLVLALLQL 

       310        320        330        340        350        360 
KDEARILRGA FLHHCIKEMH QELSECGFTD QKIRSMFIDD LLSILNIDNI HLLAEFFSFF 

       370        380        390        400        410        420 
RTFGHPILEA KVAAEKVREH MLADKVLEYA PIMKAHAIFC GTIINGYRDR HGGAWPPLYL 

       430        440        450        460        470        480 
PAHASKHIIR LKNSGESLTI DDCVKNWESF CGIQFDCFME LKLDSDLSMY MKDKALSPIK 

       490        500        510        520        530        540 
DEWDSVYPRE VLSYTPPKST EPRRLVDVFV NDENFDPYNM LEYVLSGAYL EDEQFNVSYS 

       550        560        570        580        590        600 
LKEKETKQAG RLFAKMTYKM RACQVIAEAL IASGVGKYFK ENGMVKDEHE LLKTLFQLSI 

       610        620        630        640        650        660 
SSVPRGNSQG NDPQSINNIE RDFQYFKGVT TNVKDKKNNS FNKVKSALNN PCQADGVHHN 

       670        680        690        700        710        720 
MSPNTRNRYK CSNTSKSFLD YHTEFNPHNH YKSDNTEAAV LSRYEDNTGT KFDTVSAFLT 

       730        740        750        760        770        780 
TDLKKFCLNW RYESMAIFAE RLDEIYGLPG FFNWMHKRLE RSVIYVADPN CPPNIDKHME 

       790        800        810        820        830        840 
LEKTPEDDIF IHYPKGGIEG YSQKTWTIAT IPFLFLSAYE TNTRIAAIVQ GDNESIAITQ 

       850        860        870        880        890        900 
KVHPNLPYKV KKEICAKQAQ LYFERLRMNL RALGHNLKAT ETIISTHLFI YSKKIHYDGA 

       910        920        930        940        950        960 
VLSQALKSMS RCCFWSETLV DETRSACSNI STTIAKAIEN GLSRNVGYCI NILKVIQQLL 

       970        980        990       1000       1010       1020 
ISTEFSINET LTLDVTSPIS NNLDWLITAA LIPAPIGGFN YLNLSRIFVR NIGDPVTASL 

      1030       1040       1050       1060       1070       1080 
ADLKRMIDHS IMTESVLQKV MNQEPGDASF LDWASDPYSG NLPDSQSITK TIKNITARTI 

      1090       1100       1110       1120       1130       1140 
LRNSPNPMLK GLFHDKSFDE DLELASFLMD RRVILPRAAH EILDNSLTGA REEIAGLLDT 

      1150       1160       1170       1180       1190       1200 
TKGLIRSGLR KSGLQPKLVS RLSHHDYNQF LILNKLLSNR RQNDLISSNT CSVDLARALR 

      1210       1220       1230       1240       1250       1260 
SHMWRELALG RVIYGLEVPD ALEAMVGRYI TGSLECQICE QGNTMYGWFF VPRDSQLDQV 

      1270       1280       1290       1300       1310       1320 
DREHSSIRVP YVGSSTDERS DIKLGNVKRP TKALRSAIRI ATVYTWAYGD NEECWYEAWY 

      1330       1340       1350       1360       1370       1380 
LASQRVNIDL DVLKAITPVS TSNNLSHRLR DKSTQFKFAG SVLNRVSRYV NISNDNLDFR 

      1390       1400       1410       1420       1430       1440 
IEGEKVDTNL IYQQAMLLGL SVLEGKFRLR LETDDYNGIY HLHVKDNCCV KEVADVGQVD 

      1450       1460       1470       1480       1490       1500 
AELPIPEYTE VDNNHLIYDP DPVSEIDCSR LSNQESKSRE LDFPLWSTEE LHDVLAKTVA 

      1510       1520       1530       1540       1550       1560 
QTVLEIITKA DKDVLKQHLA IDSDDNINSL ITEFLIVDPE LFALYLGQSI SIKWAFEIHH 

      1570       1580       1590       1600       1610       1620 
RRPRGRHTMV DLLSDLVSNT SKHTYKVLSN ALSHPRVFKR FVNCGLLLPT QGPYLHQQDF 

      1630       1640       1650       1660       1670       1680 
EKLSQNLLVT SYMIYLMNWC DFKKSPFLIA EQDETVISLR EDIITSKHLC VIIDLYANHH 

      1690       1700       1710       1720       1730       1740 
KPPWIIDLNP QEKICVLRDF ISKSRHVDTS SRSWNTSDLD FVIFYASLTY LRRGIIKQLR 

      1750       1760       1770       1780       1790       1800 
IRQVTEVIDT TTMLRDNIIV ENPPIKTGVL DIRGCIIYNL EEILSMNTKS ASKKIFNLNS 

      1810       1820       1830       1840       1850       1860 
RPSVENHKYR RIGLNSSSCY KALNLSPLIQ RYLPSGAQRL FIGEGSGSMM LLYQSTLGQS 

      1870       1880       1890       1900       1910       1920 
ISFYNSGIDG DYIPGQRELK LFPSEYSIAE EDPSLTGKLK GLVVPLFNGR PETTWIGNLD 

      1930       1940       1950       1960       1970       1980 
SYEYIINRTA GRSIGLVHSD MESGIDKNVE EILVEHSHLI SIAINVMMED GLLVSKIAYT 

      1990       2000       2010       2020       2030       2040 
PGFPISRLFN MYRSYFGLVL VCFPVYSNPD STEVYLLCLQ KTVKTIVPPQ KVLEHSNLHD 

      2050       2060       2070       2080       2090       2100 
EVNDQGITSV IFKIKNSQSK QFHDDLKKYY QIDQPFFVPT KITSDEQVLL QAGLKLNGPE 

      2110       2120       2130       2140       2150       2160 
ILKSEISYDI GSDINTLRDT IIIMLNEAMN YFDDNRSPSH HLEPYPVLER TRIKTIMNCV 

      2170       2180       2190       2200       2210       2220 
TKKVIVYSLI KFKDTKSSEL YHIKNNIRRK VLILDFRSKL MTKTLPKGMQ ERREKNGFKE 

      2230       2240 
VWIVDLSNRE VKIWWKIIGY ISII

Q997F0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools