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UniProtKB/Swiss-Prot entry Q97EE2

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LEU3_CLOAB
Primary accession number Q97EE2
Secondary accession numbers None
Integrated into Swiss-Prot on October 19, 2002
Sequence was last modified on October 1, 2001 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 47)
Name and origin of the protein
Protein name 3-isopropylmalate dehydrogenase
Synonyms EC 1.1.1.85
Beta-IPM dehydrogenase
IMDH
3-IPM-DH
Gene name
Name: leuB
OrderedLocusNames: CA_C3171
From
Clostridium acetobutylicum [TaxID: 1488] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787;
DOI=10.1128/JB.183.16.4823-4838.2001; PubMed=11466286 [NCBI, ExPASy, EBI, Israel, Japan]
Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R.;
"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum.";
J. Bacteriol. 183:4823-4838(2001).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE001437; AAK81108.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A97290; A97290.
RefSeq NP_349768.1; -.
3D structure databases
HSSP Q56268; 1A05. [HSSP ENTRY / PDB]
ModBase Q97EE2.
Enzyme and pathway databases
BioCyc CACE272562:CAC3171-MON; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0009098; Biological process: leucine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_01033; -; 1.
PBIL [Tree]
InterPro IPR004429; 3-isopropylmalate_DHase.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
PTHR11835:SF13; IPMDH; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00169; leuB; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS Q97EE2.
Genome annotation databases
GeneID 1119353; -.
GenomeReviews AE001437_GR; CA_C3171.
KEGG cac:CAC3171; -.
NMPDR fig|272562.1.peg.3297; -.
Phylogenomic databases
HOGENOM Q97EE2; -.
Genome annotation databases
CMR Q97EE2; CA_C3171.
Other
ProtoNet Q97EE2.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; Branched-chain amino acid biosynthesis; Complete proteome; Cytoplasm; Leucine biosynthesis; Magnesium; Manganese; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   357  357     3-isopropylmalate dehydrogenase. PRO_0000083680
NP_BIND   77    90  14     NAD (By similarity). 
NP_BIND   282   294  13     NAD (By similarity). 
METAL   224   224        Magnesium or manganese (By similarity). 
METAL   248   248        Magnesium or manganese (By similarity). 
METAL   252   252        Magnesium or manganese (By similarity). 
BINDING   97    97        Substrate (By similarity). 
BINDING   107   107        Substrate (By similarity). 
BINDING   136   136        Substrate (By similarity). 
BINDING   224   224        Substrate (By similarity). 
SITE   143   143  1     Important for catalysis (By similarity). 
SITE   192   192  1     Important for catalysis (By similarity). 
Sequence information
Length: 357 AA [This is the length of the unprocessed precursor] Molecular weight: 38980 Da [This is the MW of the unprocessed precursor] CRC64: E24DF0A2BD2EBC2C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKEYKVAVIP GDGIGVEIVG EALKVLEKVG AKYDTKFNFT EVKAGGCAID EFGVPLPNET 

        70         80         90        100        110        120 
LEICKNSDAV LLGAVGGPKW DTLPGEKRPE KALMGLRGGL GLYANLRPAK VYDILKSASP 

       130        140        150        160        170        180 
LKEEIINKGV DLLVVRELTG GIYFGERGRD IQNGINSAYD TERYNVEEIK RIAHVAFKAA 

       190        200        210        220        230        240 
LKRNKKVTSV DKANILESSR LWRETVTEVA KEYPEVELNY LYVDNAAMQL VREPSQFDVI 

       250        260        270        280        290        300 
VTSNIFGDIL TDEASMVTGS IGMLPSASLR NDTFGMYEPI HGSAPDIAGQ DLANPLAQIL 

       310        320        330        340        350 
SVAMMLEYSF NMTEAARDVE DAVEKVLNSG YRTGDIYTEG SKKVGTKEMG KLVLAEL
Q97EE2 in FASTA format

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