[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
TISSUE=Megakaryocyte, and Placenta;
DOI=10.1016/S0378-1119(00)00292-4; PubMed=11024300 [NCBI, ExPASy, EBI, Israel, Japan]
Abraham S.,
Solomon W.B.;
"A novel glutamine-rich putative transcriptional adaptor protein (TIG-1), preferentially expressed in placental and bone-marrow tissues.";
Gene 255:389-400(2000).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND POLYMORPHISM OF POLY-GLN REGION.
TISSUE=Cervix carcinoma;
DOI=10.1006/geno.2001.6566; PubMed=11414760 [NCBI, ExPASy, EBI, Israel, Japan]
Berti L.,
Mittler G.,
Przemeck G.K.H.,
Stelzer G.,
Guenzler B.,
Amati F.,
Conti E.,
Dallapiccola B.,
Hrabe' de Angelis M.,
Novelli G.,
Meisterernst M.;
"Isolation and characterization of a novel gene from the DiGeorge chromosomal region that encodes for a mediator subunit.";
Genomics 74:320-332(2001).
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[3]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
TISSUE=Hepatoma, and Spleen;
DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan]
Ota T.,
Suzuki Y.,
Nishikawa T.,
Otsuki T.,
Sugiyama T.,
Irie R.,
Wakamatsu A.,
Hayashi K.,
Sato H.,
Nagai K.,
Kimura K.,
Makita H.,
Sekine M.,
Obayashi M.,
Nishi T.,
Shibahara T.,
Tanaka T.,
Ishii S.,
Yamamoto J., ![]()
Saito K.,
Kawai Y.,
Isono Y.,
Nakamura Y.,
Nagahari K.,
Murakami K.,
Yasuda T.,
Iwayanagi T.,
Wagatsuma M.,
Shiratori A.,
Sudo H.,
Hosoiri T.,
Kaku Y.,
Kodaira H.,
Kondo H.,
Sugawara M.,
Takahashi M.,
Kanda K.,
Yokoi T.,
Furuya T.,
Kikkawa E.,
Omura Y.,
Abe K.,
Kamihara K.,
Katsuta N.,
Sato K.,
Tanikawa M.,
Yamazaki M.,
Ninomiya K.,
Ishibashi T.,
Yamashita H.,
Murakawa K.,
Fujimori K.,
Tanai H.,
Kimata M.,
Watanabe M.,
Hiraoka S.,
Chiba Y.,
Ishida S.,
Ono Y.,
Takiguchi S.,
Watanabe S.,
Yosida M.,
Hotuta T.,
Kusano J.,
Kanehori K.,
Takahashi-Fujii A.,
Hara H.,
Tanase T.-O.,
Nomura Y.,
Togiya S.,
Komai F.,
Hara R.,
Takeuchi K.,
Arita M.,
Imose N.,
Musashino K.,
Yuuki H.,
Oshima A.,
Sasaki N.,
Aotsuka S.,
Yoshikawa Y.,
Matsunawa H.,
Ichihara T.,
Shiohata N.,
Sano S.,
Moriya S.,
Momiyama H.,
Satoh N.,
Takami S.,
Terashima Y.,
Suzuki O.,
Nakagawa S.,
Senoh A.,
Mizoguchi H.,
Goto Y.,
Shimizu F.,
Wakebe H.,
Hishigaki H.,
Watanabe T.,
Sugiyama A.,
Takemoto M.,
Kawakami B.,
Yamazaki M.,
Watanabe K.,
Kumagai A.,
Itakura S.,
Fukuzumi Y.,
Fujimori Y.,
Komiyama M.,
Tashiro H.,
Tanigami A.,
Fujiwara T.,
Ono T.,
Yamada K.,
Fujii Y.,
Ozaki K.,
Hirao M.,
Ohmori Y.,
Kawabata A.,
Hikiji T.,
Kobatake N.,
Inagaki H.,
Ikema Y.,
Okamoto S.,
Okitani R.,
Kawakami T.,
Noguchi S.,
Itoh T.,
Shigeta K.,
Senba T.,
Matsumura K.,
Nakajima Y.,
Mizuno T.,
Morinaga M.,
Sasaki M.,
Togashi T.,
Oyama M.,
Hata H.,
Watanabe M.,
Komatsu T.,
Mizushima-Sugano J.,
Satoh T.,
Shirai Y.,
Takahashi Y.,
Nakagawa K.,
Okumura K.,
Nagase T.,
Nomura N.,
Kikuchi H.,
Masuho Y.,
Yamashita R.,
Nakai K.,
Yada T.,
Nakamura Y.,
Ohara O.,
Isogai T.,
Sugano S.;
"Complete sequencing and characterization of 21,243 full-length human cDNAs.";
Nat. Genet. 36:40-45(2004).
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[4]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
DOI=10.1186/gb-2004-5-10-r84; PubMed=15461802 [NCBI, ExPASy, EBI, Israel, Japan]
Collins J.E.,
Wright C.L.,
Edwards C.A.,
Davis M.P.,
Grinham J.A.,
Cole C.G.,
Goward M.E.,
Aguado B.,
Mallya M.,
Mokrab Y.,
Huckle E.J.,
Beare D.M.,
Dunham I.;
"A genome annotation-driven approach to cloning the human ORFeome.";
Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).
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[5]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 405-788 (ISOFORM 1).
TISSUE=Eye, and Kidney;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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[6]
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NUCLEOTIDE SEQUENCE [MRNA] OF 185-573 (ISOFORM 2).
TISSUE=Brain cortex;
DOI=10.1007/s004390050476; PubMed=9225980 [NCBI, ExPASy, EBI, Israel, Japan]
Margolis R.L.,
Abraham M.R.,
Gatchell S.B.,
Li S.-H.,
Kidwai A.S.,
Breschel T.S.,
Stine O.C.,
Callahan C.,
McInnis M.G.,
Ross C.A.;
"cDNAs with long CAG trinucleotide repeats from human brain.";
Hum. Genet. 100:114-122(1997).
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[7]
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IDENTIFICATION IN ARC COMPLEX, AND PROTEIN SEQUENCE OF 39-48 AND 525-536.
DOI=10.1038/19789; PubMed=10235267 [NCBI, ExPASy, EBI, Israel, Japan]
Naeaer A.M.,
Beaurang P.A.,
Zhou S.,
Abraham S.,
Solomon W.B.,
Tjian R.;
"Composite co-activator ARC mediates chromatin-directed transcriptional activation.";
Nature 398:828-832(1999).
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[8]
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FUNCTION, AND INTERACTION WITH SMAD1; SMAD2 AND SMAD3.
DOI=10.1038/nature00969; PubMed=12167862 [NCBI, ExPASy, EBI, Israel, Japan]
Kato Y.,
Habas R.,
Katsuyama Y.,
Naeaer A.M.,
He X.;
"A component of the ARC/Mediator complex required for TGF beta/Nodal signalling.";
Nature 418:641-646(2002).
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[9]
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IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE MEDIATOR COMPLEX.
DOI=10.1016/j.molcel.2004.05.006; PubMed=15175163 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S.,
Tomomori-Sato C.,
Parmely T.J.,
Florens L.,
Zybailov B.,
Swanson S.K.,
Banks C.A.S.,
Jin J.,
Cai Y.,
Washburn M.P.,
Conaway J.W.,
Conaway R.C.;
"A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.";
Mol. Cell 14:685-691(2004).
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[10]
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IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE MEDIATOR COMPLEX, AND ASSOCIATION OF THE MEDIATOR COMPLEX WITH RNA POLYMERASE II.
DOI=10.1016/j.molcel.2005.05.015; PubMed=15989967 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang X.,
Krutchinsky A.,
Fukuda A.,
Chen W.,
Yamamura S.,
Chait B.T.,
Roeder R.G.;
"MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription.";
Mol. Cell 19:89-100(2005).
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[11]
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FUNCTION.
DOI=10.1016/j.molcel.2006.03.023; PubMed=16630888 [NCBI, ExPASy, EBI, Israel, Japan]
Andrau J.-C.,
van de Pasch L.,
Lijnzaad P.,
Bijma T.,
Koerkamp M.G.,
van de Peppel J.,
Werner M.,
Holstege F.C.P.;
"Genome-wide location of the coactivator mediator: binding without activation and transient Cdk8 interaction on DNA.";
Mol. Cell 22:179-192(2006).
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[12]
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FUNCTION, INTERACTION WITH SREBF1 AND SREBF2, AND MUTAGENESIS OF GLU-42; LEU-58 AND ALA-60.
DOI=10.1038/nature04942; PubMed=16799563 [NCBI, ExPASy, EBI, Israel, Japan]
Yang F.,
Vought B.W.,
Satterlee J.S.,
Walker A.K.,
Jim Sun Z.-Y.,
Watts J.L.,
DeBeaumont R.,
Saito R.M.,
Hyberts S.G.,
Yang S.,
Macol C.,
Iyer L.,
Tjian R.,
van den Heuvel S.,
Hart A.C.,
Wagner G.,
Naeaer A.M.;
"An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis.";
Nature 442:700-704(2006).
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- FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway.
- SUBUNIT: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with SMAD2, SMAD3, SREBF1 and SREBF2.
- INTERACTION:
P16220:CREB1; NbExp=1; IntAct=EBI-394506, EBI-711855;
P10242:MYB; NbExp=1; IntAct=EBI-394506, EBI-298355;
Q9XX00:sbp-1 (xeno); NbExp=1; IntAct=EBI-394506, EBI-321652;
P36956-1:SREBF1; NbExp=4; IntAct=EBI-394506, EBI-948328;
- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
- ALTERNATIVE PRODUCTS:
3 named isoforms [FASTA] produced by alternative splicing.
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| Name | 2 |
| Isoform ID | Q96RN5-2 |
| Features which should be applied to build the isoform sequence: VSP_003922. |
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| Name | 3 |
| Isoform ID | Q96RN5-3 |
| Note: No experimental confirmation available. |
| Features which should be applied to build the isoform sequence: VSP_013024, VSP_003922. |
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- TISSUE SPECIFICITY: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney.
- INDUCTION: By 12-O-tetradecanoylphorbol-13-acetate (TPA).
- POLYMORPHISM: The poly-Gln region from amino acids 235-262 of PCQAP is polymorphic. There are from 15 to 18 repeats in the Italian population.
- SIMILARITY: Belongs to the Mediator complex subunit 15 family.
- SEQUENCE CAUTION:
- Sequence=AAC12944.1; Type=Frameshift; Positions=13, 600, 749;
- Sequence=BAB85034.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors
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